[26F3-26F3];[27A1-27A1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
26F3;27A1
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.WH5}BSC187 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. have determined the insertion site of P{XP}d10866 to be at Release 3 genomic coordinate 6701329 on chromosome arm 2L, a site predicted to be within 27A1 on both the Release 3 and Release 4 genome maps. (Note that this is not a chromosomal position predicted by the flanking sequence given in the current P{XP}d10866 insertion entry, FBti0070515.) The predicted position of PBac{WH}CG31635f04216 on the Release 4 map is 26F3. Consequently, the predicted cytological breakpoints of Df(2L)BSC187 are 26F3;27A1.