Df(1)LIMK1-5 mutants have slightly smaller muscle fibers than wild type and the mutant NMJs have an expanded morphology compared to wild type. The Df(1)LIMK1-5 NMJs exhibit increased bouton number (by 73-112%). However, the function of Df(1)LIMK1-5 NMJs is normal, as judged by evoked Excitatory Potentials (mEJPs). Df(1)LIMK1-5 NMJs have slightly fewer active zones per bouton than wild type, suggesting that it is this compensatory mechanism that makes the function normal.

The patterns of axon trajectories in both the CNS and PNS are normal in Df(1)LIMK1-5 mutants. The differentiation of the olfactory receptors and the targeting of their axons is normal in these mutants.

Expression of Pak^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^SG18.1 in a Df(1)LIMK1-5 background suppresses the antennal lobe anatomy phenotype seen when this transgene is expressed in a wild type background.