[86C7-86C7];[86D7-86D7];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
86C7;86D7
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(3R)ED5518.
Inferred to overlap with: Df(3R)Exel7306.
The presence of P+PBac{XP5.RB3}BSC568 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3R)BSC568 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CG6574e00293 and P{XP}d10159 transposable element insertion sites are 86C7;86D7.