A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC809 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in FBrf0174229.
The breakpoints of Df(3R)BSC809 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d10097 and PBac{RB}CG4662e00391 insertion sites are 3R:15229135 ;15672723 and the cytological breakpoints predicted from these coordinates are 92A3;92B3.