A set of ~400 overlapping Pacman BAC clones was used to create small duplications (average length 88 kb) covering most of the 22-Mb sequenced portion of the X chromosome. Using the Pacman system (FBrf0194996), the BAC clones were inserted into an attP docking site on chromosome arm 3L; available as homozygous or balanced heterozygous stocks.
Docking-site insertion on 2L used for one line.
A duplication of sequence from the X chromosome derived from the CH321-48H18 BAC clone has been inserted into the PBac{y+-attP-3B}VK00033 docking site on the 3rd chromosome using the P"["acman"]" system. The left end of the duplicated sequence is in a tandem repeat, the coordinate is most likely 1:7710486 , release 5 but it could end at 1:7711560 , release 5. The right end of the duplication is at coordinate: 1:7803844 , release 5.
Homozygous viable but homozygous males have reduced fertility.