FlyBase Allele Report: Dmel\ci[Ce-2]

General Information

Symbol

Dmel\ci^Ce-2

Species

D. melanogaster

Name

FlyBase ID

FBal0001657

Feature type

allele

Associated gene

Dmel\ci

Associated Insertion(s)

Carried in Construct

Also Known As

Ce², ci^Cell, ci^Ce, Ci^cell2, ci^ce2

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Green, 1952)

Progenitor genotype

Cytology

Description

8bp deletion that is expected to result in a truncation of the protein at amino acid residue 975.

(Methot and Basler, 1999)

Two insertions, 3.0kb insertion in the HaeIII fragment and 2.6kb insertion in the BglII fragment.

(Locke and Tartof, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Locke and Tartof, 1994, Orenic et al., 1987, Hochman, 1976, Green, 1952)

lethal - all die during embryonic stage | recessive

(Eaton and Kornberg, 1990)

segment polarity phenotype

some die during embryonic stage | recessive

(Eaton and Kornberg, 1990)

visible | dominant

(Methot and Basler, 1999, Locke and Hanna, 1996, Green, 1952)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous embryos have a stronger segment polarity phenotype than ci⁹⁴ embryos, resembling more closely the phenotype of hh mutant embryos.

(Methot and Basler, 2001)

In the wing disc, 13 out of 25 anterior ci^Ce-2/ci⁹⁴ clones that originate in the vicinity of the compartment boundary sort entirely within the normal posterior territory. These clones define straight borders to anterior cells at the normal position of the anterior/posterior boundary. ci^Ce-2/ci⁹⁴ clones of posterior origin remain exclusively in the posterior territory.

(Dahmann and Basler, 2000)

Denticle diversity is still seen in mutant embryos.

(Gallet et al., 2000)

Bolwig's organs are missing in mutant embryos.

(Suzuki and Saigo, 2000)

Heterozygotes show partial fusion of wing veins L3 and L4 in the distal and proximal parts of the wing and concomitant loss of some L3/L4 intervein material.

(Methot and Basler, 1999)

When wg^Scer\UAS.cLa is driven by Scer\GAL4^en-e16E in a ci^Ce-2 embryo, denticle rows 2, 3 and 4 are replaced by naked cuticle. Rows 5 and 6 are replaced by small denticles. In heterozygotes for ci^Ce-2, carrying wg^Scer\UAS.cLa and Scer\GAL4^en-e16E, occasional breaches in the normal denticle belts occur as patches of naked cuticle, revealing a dosage sensitivity to the requirement for hh function at the segment border.

(Sanson et al., 1999)

Naked cuticle is deleted and the ventral surface consists of a lawn of denticles. ptc⁹; ci^Ce-2 double mutants phenotypically resemble ci^Ce-2 single mutants.

(Motzny and Holmgren, 1995)

Segment polarity phenotype.

(Eaton and Kornberg, 1990)

Certain embryonic neurons along the midline have a fate modification.

(Patel et al., 1989)

Pupal lethal in combination with ci^D. RK3.

(Hochman, 1976)

Ocelli are reduced or absent and ocellar and scutellar bristles are absent in heterozygotes. Wing veins L3 and L4 converge, giving a phenotype much like fu^unspecified, although the wing phenotype is variable.

(Green, 1952)

Ocelli reduced or absent; ocellar and scutellar bristles absent; interocellar microchaetae disrupted but frontals normal; postverticals short, thick, often with an adventitious pair between the normally placed postvertical bristles. Wing veins L3 and L4 converge, giving wing phenotype much like fu although wing phenotype variable. Homozygous lethal; lethality occurs during embryonic period (Hochman). Shown to be a segment polarity gene; homozygous embryos exhibit loss of the naked cuticle in the posterior half of each segment plus the anterior margin of the adjacent segment; most dorsal pattern elements also eliminated, leaving a lawn of fine hairs. Engrailed-antibody staining fails to detect a subset of CNS neurons in ci homozygotes that are normally stained in wild-type embryos (FBrf0049834).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

ci^Ce-2, fu^unspecified has lethal | larval stage phenotype

(Alves et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

ci^Ce-2 has Bolwig organ phenotype, suppressible by hh^UAS.cKa/Scer\GAL4^da.G32

(Suzuki and Saigo, 2000)

Other

Statement

Reference

ci^Ce-2, fu^unspecified has imaginal disc phenotype

(Alves et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The ci^Ce-2 Bolwig's organ phenotype is considerably rescued by expression of hh^Scer\UAS.cKa under the control of Scer\GAL4^da.G32.

(Suzuki and Saigo, 2000)

fu^unspecified; ci^Ce-2/+ animals die during the third larval instar stage and have very small imaginal discs.

(Alves et al., 1998)

When doubly heterozygous with en loss of function alleles, the dominant ci phenotype is enhanced.

(Locke and Hanna, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

ci⁵⁷