Deletion beginning at amino acid 65 and extending towards the C terminal of the protein.
dsx9/dsx1, ix3 has abdominal tergite 6 | female phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has abdominal sternite 6 | male phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has cercus phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has epiproct phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has prothoracic metatarsus phenotype
Df(2R)en-B/ix3, dsx9 has abdominal tergite 6 | female phenotype
Df(2R)en-B/ix3, dsx9 has hypogynial tooth phenotype
Df(2R)en-B/ix3, dsx9 has sex comb phenotype
Df(2R)en-B/ix3, dsx9 has abdominal sternite 6 | male phenotype
Df(2R)en-B/ix3, dsx9 has cercus phenotype
dsx9/dsx1, ix3 has hypogynial tooth phenotype
Df(2R)en-B/ix3, dsx9 has epiproct phenotype
Df(2R)en-B/ix3, dsx9 has prothoracic metatarsus phenotype
dsx9/dsx1, ix3 has abdominal sternite 6 phenotype
Df(2R)en-B/+, dsx9/dsx1 has abdominal tergite 6 | female phenotype
Df(2R)en-B/+, dsx9/dsx1 has hypogynial tooth phenotype
Df(2R)en-B/+, dsx9/dsx1 has abdominal sternite 6 phenotype
Df(2R)en-B/+, dsx9/dsx1 has epiproct phenotype
Df(2R)en-B/+, dsx9/dsx1 has prothoracic metatarsus | female phenotype
dsx9/dsx1, ix3/ix[+] has abdominal tergite 6 | female phenotype
dsx9/dsx1, ix3/ix[+] has hypogynial tooth phenotype
dsx9/dsx1, ix3/ix[+] has abdominal sternite 6 phenotype
dsx9/dsx1, ix3/ix[+] has prothoracic metatarsus | female phenotype
dsx9/dsx1, ix3 has prothoracic metatarsus | female phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has abdominal tergite 6 | female phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has hypogynial tooth phenotype
Df(2R)en-B/ix3, dsx9/dsx1 has sex comb phenotype
ix; dsx transheterozygous progeny from ix3/+; dsx1/+ females crossed to Df(2R)en-B/+; dsx9/+
males
show little or no masculinisation of females, or feminisation of males,
as judged by: Mean number of vaginal teeth; percentage of females with
fused dorsal-lateral anal plates; percentage width of the 6th tergite
that is darkly pigmented; number of bristles in the last transverse
row in the foreleg basitarsus (note this row forms the sex comb in
males); or number of bristles on the sixth sternite.
ix3/Df(2R)en-B; dsx9/dsx1 double mutants show masculinisation
of females, as judged by the following criteria:
The mean number of vaginal teeth is reduced to 6.45 +/- 2.11. 85 %
of females have fused dorsal-lateral anal plates (as wild type; note
that even those with fused plates exhibit variable degrees of partial
fusion as judged against fusion in wild-type females). The mean percentage
width of the 6th tergite that is darkly pigmented is increased to 95%
(+/- 0.4%). The number of bristles in the last transverse row in the
foreleg basitarsus is increased to: 7.25 +/- 0.54 ( this row forms
the sex comb in males).
ix3/Df(2R)en-B; dsx9/dsx1 double mutant females do not
show significant reductions in the number of bristles on the sixth
sternite (20.75 +/- 1.83).
ix3/Df(2R)en-B; dsx9/dsx1 double mutants show feminisation
of males, as judged by the following criteria:
An average of 6.85 (+/- 2.74) ectopic vaginal teeth form.
Fusion of dorso-lateral anal plates is seen in 80% of males.
The number of bristles in the last transverse row in the foreleg basitarsus
is decreased to 7.25 +/-0.54 (this row forms the sex comb in males).
The average number of bristles on the sixth sternite increases dramatically
from near zero to 18.2 +/- 1.58.
ix3/Df(2R)en-B; dsx9/dsx1 double mutant males do not
show significant masculinisation of pigmentation of the 6th tergite,
which remains
96% (+/-3%) darkly pigmented.
ix3/Df(2R)en-B mutant females heterozygous for dsx9 or dsx1
show masculinisation, as judged by the following criteria:
Mean number of vaginal teeth is reduced to 9.7 +/- 5.29. 90 % of females
have fused dorsal-lateral anal plates (as wild type; note that even
those with fused plates exhibit variable degrees of partial fusion
as judged against fusion in wild-type females). The mean percentage
width of the 6th tergite that is darkly pigmented is increased to 92%
(+/-6%). The number of bristles in the last transverse row in the foreleg
basitarsus is increased to: 6.88 +/- 0.65 (this row forms the sex comb
in males).
ix3/Df(2R)en-B mutant females transheterozygous for dsx9/+
or dsx1/+ do not show significant reductions in the number of bristles
on the sixth sternite (21.1 +/-1.68)
ix3/Df(2R)en-B mutant males heterozygous for dsx9 or dsx1
do not show feminisation, as judged by the following criteria:
Mean number of vaginal teeth; percentage of males with fused dorsal-lateral
anal plates; percentage width of the 6th tergite that is darkly pigmented;
number of bristles in the last transverse row in the foreleg basitarsus
(note this row forms the sex comb in males); or number of bristles
on the sixth sternite.
dsx9/dsx1 mutant females heterozygous for ix3 or Df(2R)en-B
show masculinisation, as judged by the following criteria:
Mean number of vaginal teeth is reduced to 6.0 +/-3.55. 65 % of females
have fused dorsal-lateral anal plates (as wild type; note that even
those with fused plates exhibit variable degrees of partial fusion
as judged against fusion in wild-type females). The mean percentage
width of the 6th tergite that is darkly pigmented is increased to 96%
(+/-5%). The number of bristles in the last transverse row in the foreleg
basitarsus is increased to: 7.75 +/- 0.78 (this row forms the sex comb
in males). The average number of bristles on the sixth sternite is
slightly decreased to 19.5 +/- 1.91.
dsx9/dsx1 mutant males heterozygous for ix3 or Df(2R)en-B
show feminisation, as judged by the following criteria:
An average of 6 (+/- 4.19) ectopic vaginal teeth form. Fusion of dorso-lateral
anal plates is seen in 70% of males. The number of bristles in the
last transverse row in the foreleg basitarsus is decreased to 7.45
+/-0.6 (this row forms the sex comb in males). The average number
of bristles on the sixth sternite increases dramatically from near
zero to 17.3 +/- 2.06.
dsx9/dsx1 mutant males heterozygous for ix3 or Df(2R)en-B
do not show significant masculinisation of pigmentation of the 6th
tergite, which remains 95% (+/-4%) darkly pigmented.
Garen.
No sequence specific DNA binding activity by encoded product in gel mobility shift assays.