Open Close
General Information
Symbol
Dmel\ea1
Species
D. melanogaster
Name
FlyBase ID
FBal0003455
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G15330197A

Reported nucleotide change:

G1218A

Amino acid change:

G339R | ea-PA; G208R | ea-PB

Reported amino acid change:

G339R

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Point mutation in the catalytic site.

Amino acid replacement: G339R.

Nucleotide substitution: G1218A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of ea1/ea3 larvae is statistically indistinguishable from that of control siblings. The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of homozygous larvae is significantly lower than that of control siblings. The ability of ea1/ea3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.

Bacterially challenged mutants exhibit a wild type Drs response. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of ea1/ea2 mutants with A.fumigatus causes higher survival rate than wild type, infection with E.coli causes 87% survival 3 days postinfection.

Heterozygotes are female sterile.

Injection of snkΔne.T:ea into the central region of homozygous embryos causes a dorsalized phenotype.

Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements. Perivitelline fluid transplantation using eaD8 embryos as donors and ea1/ea2 embryos as recipients demonstrated that the rescue of the ea phenotype results from the transplantation of the ea gene product.

Does not interact with RpII140wimp maternal effect.

Ventralized embryos: rings or patches of ventral denticles along dorsoventral axis. Altered pattern of dpp and zen.

Embryos strongly dorsalized.

Homozygous embryos have a strongly dorsalized phenotype: dorsal cuticle differentiates around the entire dorso-ventral circumference. ea1/ea7 heterozygotes at 18oC have a moderately dorsalized phenotype: very reduced denticle belts. ea1/ea3 heterozygotes at 18oC have a weakly dorsalized phenotype: ventral denticle belts are of nearly normal width and there are abnormalities in the head skeleton.

strong allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference

ea1/ea[+] is an enhancer of embryonic epidermis phenotype of cactE10

Additional Comments
Genetic Interactions
Statement
Reference

Embryos derived from Spn27Aex32/Df(2L)BSC7 ; ea1/ea2 females are completely dorsalised.

Perivitelline fluid from embryos laid by eaD3 spz4/ea1 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females.

50% of embryos produced by cactE10/+, ea1/+ are weakly dorsalized.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (25)