The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of ea1/ea3 larvae is statistically indistinguishable from that of control siblings. The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of homozygous larvae is significantly lower than that of control siblings. The ability of ea1/ea3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.
Bacterially challenged mutants exhibit a wild type Drs response. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of ea1/ea2 mutants with A.fumigatus causes higher survival rate than wild type, infection with E.coli causes 87% survival 3 days postinfection.
Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements. Perivitelline fluid transplantation using eaD8 embryos as donors and ea1/ea2 embryos as recipients demonstrated that the rescue of the ea phenotype results from the transplantation of the ea gene product.
Homozygous embryos have a strongly dorsalized phenotype: dorsal cuticle differentiates around the entire dorso-ventral circumference. ea1/ea7 heterozygotes at 18oC have a moderately dorsalized phenotype: very reduced denticle belts. ea1/ea3 heterozygotes at 18oC have a weakly dorsalized phenotype: ventral denticle belts are of nearly normal width and there are abnormalities in the head skeleton.