FlyBase Allele Report: Dmel\elav[ts1]

General Information

Symbol

Dmel\elav^ts1

Species

D. melanogaster

Name

FlyBase ID

FBal0003650

Feature type

allele

Associated gene

Dmel\elav

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Samson et al., 1995)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Samson et al., 1995)

Amino acid replacement: W419term.

(Samson et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:517,988..517,988 [-]

Nucleotide change:

G517988A

Reported nucleotide change:

G?A

Amino acid change:

W419term | elav-PA; W415term | elav-PB; W415term | elav-PC; W419term | elav-PD

Reported amino acid change:

W419term

(Samson et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Campos et al., 1985)

abnormal behavior | heat sensitive

(Samson et al., 1995)

abnormal locomotor behavior | adult stage (with elav⁵)

(Zaharieva et al., 2015)

abnormal neuroanatomy | embryonic stage (with elav⁵)

(Zaharieva et al., 2015)

abnormal neuroanatomy | heat sensitive (with elav⁵)

(Haussmann et al., 2008)

abnormal neuroanatomy | third instar larval stage | conditional (with elav⁵)

(Zaharieva et al., 2015)

flightless | heat sensitive

(Samson et al., 1995)

lethal | heat sensitive

(Lisbin et al., 2000, Campos et al., 1985)

lethal | heat sensitive (with elav⁵)

(Haussmann et al., 2008)

partially lethal - majority die | temperature conditional (with elav⁵)

(Zaharieva et al., 2015)

short lived (with elav⁵)

(Zaharieva et al., 2015)

uncoordinated | heat sensitive

(Samson et al., 1995)

viable

(Samson et al., 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

The neuromuscular junctions of elav⁵/elav^ts1 mutant third instar larvae (raised at the permissive temperature during embryonic development) show a strong reduction in the number of synaptic connections compared with controls.

elav⁵/elav^ts1 adult flies do not exhibit any fusion of the mushroom body beta lobes.

elav⁵/elav^ts1 are short lived, show reduced locomotion and are ataxic.

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals show a significant reduction in the number of type 1b boutons at the neuromuscular junction of muscle 13 when they are reared at the restrictive temperature during larval life.

(Haussmann et al., 2008)

elav^ts1 flies survive at 22^oC but not at 25^oC, although a low percentage of survivors are seen depending on the culture conditions.

(Lisbin et al., 2000)

Morphology of visual system is abnormal at 18^oC and worse at 25^oC.

(Samson et al., 1995)

elav^ts1 optic chiasma is abnormal and second order optic lobe (medulla) is rotated to aberrant position. When elav^ts1 raised at 30^oC, surface of eye is rough and photoreceptor layer abnormal in sections. At 19^oC 63% survive to adult hood, at 30^oC 2% survive to adulthood. Surviving adults have poor coordination: neural defects are not restricted to the visual system.

(Campos et al., 1985)

temperature-sensitive

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

elav⁵/elav^ts1 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(1)fne^Δ

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Rbp9^P2690/Df(2L)ED206

(Zaharieva et al., 2015)

Suppressed by

Statement

Reference

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav^ts1 has lethal phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav^ts1 has lethal phenotype, suppressible by Rbp9^{elav.Tag:NLS(Unk).Tag:HA}

(Zaharieva et al., 2015)

elav^ts1 has lethal phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav^ts1 has lethal phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has lethal | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵/elav^ts1 has abnormal neuroanatomy | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Rbp9^ER3

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Sxl^ES1.2

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Sxl^ES2

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Sxl^ES1

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Rbp9^ER1

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, suppressible by elav::Hsap\ELAVL4^EH3

(Lisbin et al., 2000)

NOT suppressed by

Statement

Reference

elav^ts1 has lethal | heat sensitive phenotype, non-suppressible by elav::Rbp9^ER12

(Lisbin et al., 2000)

elav^ts1 has lethal | heat sensitive phenotype, non-suppressible by elav::Sxl^ES12

(Lisbin et al., 2000)

Other

Statement

Reference

Df(1)fne^Δ, elav⁵/elav^ts1 has lethal - all die before end of pupal stage phenotype

(Zaharieva et al., 2015)

Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die before end of pupal stage phenotype

(Zaharieva et al., 2015)

Df(1)fne^Δ, Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die during embryonic stage phenotype

(Zaharieva et al., 2015)

elav⁵/elav^ts1, ewg^SC3ORF.elav has flightless phenotype

(Haussmann et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Df(1)fne^Δ

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Rbp9^P2690/Df(2L)ED206

(Zaharieva et al., 2015)

Suppressed by

Statement

Reference

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵/elav^ts1 has neuromuscular junction | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Rbp9^P2690/Df(2L)ED206 does not enhance the neuromuscular junction phenotypes seen in elav⁵/elav^ts1 mutant third instar larvae.

Expression of Rbp9^{elav.T:Ivir\HA1} partially rescues the viability of elav^ts1. Expression of Rbp9^{elav.T:Uuuu\nls-Unk.T:Ivir\HA1} rescues the viability to a greater extent, comparable to the rescue by elav^T:Ivir\HA1.

Df(1)fne^Δ does not enhance the neuromuscular junction phenotypes seen in elav⁵/elav^ts1 mutant third instar larvae.

Expression of fne^{elav.T:Ivir\HA1} partially rescues the viability of elav^ts1.

Expression of fne^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

Expression of Rbp9^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals eclose as adults when they are rescued by ewg^elav.NS (providing that embryogenesis occurs at the permissive temperature for the elav^ts1 allele). However, the rescued adults show motor defects and are flightless.

The reduction in bouton number at the neuromuscular junction which is seen in elav^ts1/elav⁵ animals reared at the restrictive temperature during larval life is rescued by ewg^elav.NS.

(Haussmann et al., 2008)

One transgenic line of elav::Sxl^ES1 shows slight rescue of elav⁵ (8% rescue).

(Lisbin et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ELAVL1^{elav.T:Ivir\HA1} partially rescues the viability of elav^ts1.

Expression of Hsap\ELAVL1^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

(Zaharieva et al., 2015)

Complementation and Rescue Data

Rescued by

elav⁵/elav^ts1 is rescued by elav^Tag:HA

(Zaharieva et al., 2015)

elav^ts1 is rescued by elav^Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 is rescued by elav^DmORF

(Haussmann et al., 2008)

elav^ts1 is rescued by elav^DmORF

(Lisbin et al., 2000)

elav^ts1 is rescued by elav^-13

(Lisbin et al., 2000)

Partially rescued by

elav^ts1 is partially rescued by elav^3AGD

(Lisbin et al., 2000)

elav^ts1 is partially rescued by elav^ΔOh

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^DmORF

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^{ΔNh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^ΔNh

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^ΔCh

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^{ΔCh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^{ΔEh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav^ts1 is partially rescued by elav^{ΔOh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

Not rescued by

elav^ts1 is not rescued by elav^2AGD

(Lisbin et al., 2000)

elav^ts1 is not rescued by elav^1AGD

(Lisbin et al., 2000)

elav^ts1 is not rescued by elav^ΔEh

(Yannoni and White, 1999)

Comments

Expression of elav^T:Ivir\HA1 rescues the viability of elav^ts1.

Expression of elav^T:Ivir\HA1 rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant flies (raised at the permissive temperature during embryonic development).

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals eclose as adults when they are rescued by elav^DmORF (providing that embryogenesis occurs at the permissive temperature for the elav^ts1 allele).

The reduction in bouton number at the neuromuscular junction which is seen in elav^ts1/elav⁵ animals reared at the restrictive temperature during larval life is rescued by elav^DmORF.

(Haussmann et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605952

y¹ elav^ts1 w¹ sn³/FM6, w^*

Notes on Origin

Discoverer

White.

Comments

Analysis of protein product reveals full length as well as truncated elav protein.

(Samson et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

elav^ts1

(Zaharieva et al., 2015, Haussmann et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (6)

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