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Citation
Zaharieva, E., Haussmann, I.U., Bräuer, U., Soller, M. (2015). Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.  Mol. Cell. Biol. 35(18): 3104--3115.
FlyBase ID
FBrf0229345
Publication Type
Research paper
Abstract

Neuronally coexpressed ELAV/Hu proteins comprise a family of highly related RNA binding proteins which bind to very similar cognate sequences. How this redundancy is linked to in vivo function and how gene-specific regulation is achieved have not been clear. Analysis of mutants in Drosophila ELAV/Hu family proteins ELAV, FNE, and RBP9 and of genetic interactions among them indicates that they have mostly independent roles in neuronal development and function but have converging roles in the regulation of synaptic plasticity. Conversely, ELAV, FNE, RBP9, and human HuR bind ELAV target RNA in vitro with similar affinities. Likewise, all can regulate alternative splicing of ELAV target genes in nonneuronal wing disc cells and substitute for ELAV in eye development upon artificially increased expression; they can also substantially restore ELAV's biological functions when expressed under the control of the elav gene. Furthermore, ELAV-related Sex-lethal can regulate ELAV targets, and ELAV/Hu proteins can interfere with sexual differentiation. An ancient relationship to Sex-lethal is revealed by gonadal expression of RBP9, providing a maternal fail-safe for dosage compensation. Our results indicate that highly related ELAV/Hu RNA binding proteins select targets for mRNA processing through alteration of their expression levels and subcellular localization but only minimally by altered RNA binding specificity.

PubMed ID
PubMed Central ID
PMC4539368 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

UAS-gene insertions published in Zaharieva et al. 2015 (FBrf0229345).
Soller, 2018.2.14, UAS-gene insertions published in Zaharieva et al. 2015 (FBrf0229345). [FBrf0238060]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell. Biol.
    Title
    Molecular and Cellular Biology
    Publication Year
    1981-
    ISBN/ISSN
    0270-7306
    Data From Reference
    Aberrations (3)
    Alleles (30)
    Genes (18)
    Natural transposons (2)
    Insertions (5)
    Experimental Tools (6)
    Transgenic Constructs (26)