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General Information
Symbol
Dmel\esc21
Species
D. melanogaster
Name
FlyBase ID
FBal0003841
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C11827836T

Amino acid change:

S301F | esc-PA

Reported amino acid change:

S301F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: S301F. S301F falls in the WD5 domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dendrite & dorsal multidendritic neuron ddaC | somatic clone

dendrite & dorsal multidendritic neuron ddaC | third instar larval stage

Detailed Description
Statement
Reference

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for esc21 show a range of dendritic defects in the third instar larva. Homozygous class IV ddaC clones show a significant reduction in dendrite branching and total dendritic length compared to controls.

Homozygotes can survive through larval stages and often into adulthood.

Dendrites of class IV dendrite arborisation (da) neurons are indistinguishable from wild-type controls in homozygotes throughout embryogenesis and early larval stages. However, beginning at 48 hours after egg laying (AEL), defects in class IV dendrites become evident in homozygotes. By 96 hours AEL there is a significant reduction both in the number of terminal dendritic branches and in total dendritic length in the homozygotes. In some severe cases (approximately 10% of neurons sampled), there is a complete loss over major regions of the dendritic field. Analysis of dendrite development between 72 and 96 hours AEL shows that homozygous class IV dendrites show extensive growth during this period, as occurs in controls, but in the mutants, some major dendritic trunks show signs of severing (which is never seen in controls). By 96 hours AEL, the severed dendrites are almost completely eliminated in the mutants. Mutant dendrites show a greater number and apparent rate of retraction events than controls.

Class IV da neurons show no sign of cell death in mutant third instar larvae and they persist through metamorphosis and into adulthood.

Class IV da neurons enter the central nervous system at the appropriate position in mutant larvae and the overall morphology of the neurons in the central nerve cord is indistinguishable from that of controls.

Heterozygous males do not have ectopic sex combs on the second or third legs.

Heterozygous males do not have ectopic sex combs on the second and third legs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

esc21/esc[+] is an enhancer of visible | dominant phenotype of Pc3

NOT Enhancer of
Statement
Reference

esc21/esc[+] is a non-enhancer of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

esc21/esc[+] is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of Pc15

esc21/esc[+] is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of E(z)63

Suppressor of
Statement
Reference

esc21/esc[+] is a suppressor | partially of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4ptc-559.1

esc21/esc[+] is a suppressor of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4GMR.PU

esc21/esc[+] is a suppressor of visible | dominant | homeotic phenotype of brm[+]/brm2, trxE2

esc21/esc[+] is a suppressor of visible | dominant | homeotic phenotype of ash117, trxB11/trx[+]

NOT Suppressor of
Statement
Reference

esc21/esc[+] is a non-suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

esc21/esc[+] is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of Pc15

esc21/esc[+] is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of E(z)63

Phenotype Manifest In
Enhancer of
Statement
Reference

esc21/esc[+] is an enhancer of sex comb | ectopic phenotype of Pc3

esc21/esc[+] is an enhancer of mesothoracic leg phenotype of Pc3

esc21/esc[+] is an enhancer of metathoracic leg phenotype of Pc3

NOT Enhancer of
Statement
Reference

esc21/esc[+] is a non-enhancer of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

esc21/esc[+] is a non-enhancer of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of Pc15

esc21/esc[+] is a non-enhancer of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of E(z)63

Suppressor of
Statement
Reference

esc21/esc[+] is a suppressor of eye phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4GMR.PU

esc21/esc[+] is a suppressor of adult metathoracic segment phenotype of brm[+]/brm2, trxE2

esc21/esc[+] is a suppressor of adult metathoracic segment phenotype of ash117, trxB11/trx[+]

NOT Suppressor of
Statement
Reference

esc21/esc[+] is a non-suppressor of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

esc21/esc[+] is a non-suppressor of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of Pc15

esc21/esc[+] is a non-suppressor of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of E(z)63

Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is not affected by combination with a single copy of esc21.

The addition of esc21/+ does not alter the dendritic defects seen in single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Pc15 or homozygous for E(z)63.

The ectopic sex comb phenotype seen on the second and third legs of Pc3/+ males is enhanced by esc21/+.

rept06945 shows little or no interaction (in terms of ectopic sex comb phenotype) when in double heterozygous combination with esc21.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is not affected by combination with a single copy of esc21.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Struhl.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (16)