FlyBase Allele Report: Dmel\E(z)[63]

General Information

Symbol

Dmel\E(z)⁶³

Species

D. melanogaster

Name

FlyBase ID

FBal0003442

Feature type

allele

Associated gene

Dmel\E(z)

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Carrington and Jones, 1996, Jones and Gelbart, 1990)

loss of function allele

Mutagen

ethyl methanesulfonate

(Jones and Gelbart, 1990)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Jones and Gelbart, 1990)

Description

Amino acid replacement: M1I. Nucleotide substitution: G106A. Eliminates the translation initiation codon.

(Carrington and Jones, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:10,637,759..10,637,759 [-]

Nucleotide change:

G10637759A

Reported nucleotide change:

G106A

Amino acid change:

M1I | E(z)-PA; M1I | E(z)-PB; M1I | E(z)-PC

Reported amino acid change:

M1I

(Carrington and Jones, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Song et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage | dominant

(Lv et al., 2024)

abnormal mitotic cell cycle | embryonic stage

(O'Dor et al., 2006)

abnormal neuroanatomy | somatic clone

(Parrish et al., 2007)

bang sensitive (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

fertile (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

fertile (with E(z)⁷³¹), with E(z)^{αTub84B.EGFP}

(Jangam et al., 2023)

increased cell death | larval stage | somatic clone

(Beuchle et al., 2001)

lethal (with E(z)⁷³¹)

(Jangam et al., 2023)

lethal | recessive

short lived (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

short lived (with E(z)⁷³¹), with E(z)^{αTub84B.EGFP}

(Jangam et al., 2023)

Phenotype Manifest In

abdominal tergite 4 (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

dendrite & dorsal multidendritic neuron ddaC | somatic clone

(Parrish et al., 2007)

leg (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

metaphase & condensed chromosome & embryo

(O'Dor et al., 2006)

sex comb | adult stage | ectopic (with E(z)⁷³¹), with E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

wing disc | somatic clone

(Beuchle et al., 2001)

Detailed Description

Statement

Reference

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for E(z)⁶³ show a range of defects in the third instar larva. Homozygous class IV ddaC clones fail to properly cover the receptive field and show a significant reduction in dendrite branching, with on average a greater than 50% reduction in dendrite branch points and total dendritic length compared to controls. Terminal dendrite branches are affected more severely in the mutant neurons than major dendritic branches, and as a results the receptive field of mutant clones covers only 75% of the space occupied by wild-type control clones. Neighbouring heterozygous class IV neurons show very little invasion into the unoccupied territory left by the homozygous clone cell.

Axons of mutant single cell ddaC neuron clones occupy a similar fascicular position in the ventral nerve cord and show very similar arborisation patterns as wild-type controls. Axon terminals of mutant v'ada or vdab clones are also indistinguishable from wild type.

Heterozygous larvae show no significant defects in dendrite morphology of class IV dendrite arborisation (da) neurons.

(Parrish et al., 2007)

Early E(z)⁶³ embryos from heterozygous mothers exhibit significantly under-condensed chromatin at metaphase, causing the metaphase plate to increase from the wild-type width of ~2μm to ~5μm. These embryos exhibit chromatid arms that do not properly align at the metaphase plate, but instead project outwards. These embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. This nuclear fallout is not severe and is often observed at the metaphase stage.

(O'Dor et al., 2006)

Homozygous mutant clones in the wing disc appear to be eliminated by cell competition if not given a proliferation advantage (such as using the Minute technique).

(Beuchle et al., 2001)

The total level of hydrocarbons and the level of 7-monoenes is significantly reduced in heterozygous males compared to controls. The total level of hydrocarbons and the level of 7,11-dienes is significantly decreased in heterozygous females compared to controls.

(Wicker-Thomas and Jallon, 2000)

Hemizygous mxc^M1 males show weak homeotic phenotypes. No significant interaction is detected in mxc^M1/Y in trans with one mutant copy of E(z)⁶³.

(Saget et al., 1998)

Dominant suppressor of z mutants.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

E(z)⁶³ has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by esc²¹/esc[+]

(Parrish et al., 2007)

NOT suppressed by

Statement

Reference

E(z)⁶³ has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by esc²¹/esc[+]

(Parrish et al., 2007)

Suppressor of

Statement

Reference

E(z)[+]/E(z)⁶³ is a suppressor of abnormal circadian rhythm | dominant | adult stage phenotype of CoRest^MI08173

(Lv et al., 2024)

Other

Statement

Reference

E(z)⁶³, Su(z)12⁴/Su(z)12[+] has abnormal neuroanatomy | dominant phenotype

(Parrish et al., 2007)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

E(z)⁶³ has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype, non-enhanceable by esc²¹/esc[+]

(Parrish et al., 2007)

E(z)⁶³ has sex comb phenotype, non-enhanceable by HDAC1⁰⁴⁵⁵⁶

(Chang et al., 2001)

NOT suppressed by

Statement

Reference

E(z)⁶³ has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype, non-suppressible by esc²¹/esc[+]

(Parrish et al., 2007)

E(z)⁶³ has sex comb phenotype, non-suppressible by HDAC1⁰⁴⁵⁵⁶

(Chang et al., 2001)

NOT Enhancer of

Statement

Reference

E(z)⁶³ is a non-enhancer of sex comb phenotype of HDAC1⁰⁴⁵⁵⁶

(Chang et al., 2001)

Suppressor of

Statement

Reference

E(z)⁶³ is a suppressor of phenotype of z¹

NOT Suppressor of

Statement

Reference

E(z)⁶³ is a non-suppressor of sex comb phenotype of HDAC1⁰⁴⁵⁵⁶

(Chang et al., 2001)

Other

Statement

Reference

E(z)⁶³, Su(z)12⁴/Su(z)12[+] has dendrite & dorsal multidendritic neuron ddaC phenotype

(Parrish et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of esc²¹/+ does not alter the dendritic defects seen in single cell class IV dendrite arborisation (da) neuron clones that are homozygous for E(z)⁶³.

Pc¹/E(z)⁶³ double heterozygous larvae show dendritic defects in class IV dendrite arborisation (da) neurons; there is a significant reduction in the number of dendritic branchpoints.

(Parrish et al., 2007)

The addition of esc¹⁰ or E(z)⁶³ to Rpd3⁰⁴⁵⁵⁶ animals has no effect on the extra sex comb phenotype.

(Chang et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

E(z)⁷³¹/E(z)⁶³ is rescued by E(z)^{αTub84B.EGFP}

(Jangam et al., 2023)

E(z)⁷³¹/E(z)⁶³ is rescued by E(z)^{A691G.αTub84B.EGFP}

(Jangam et al., 2023)

E(z)⁶³ is rescued by E(z)^+t8.8

(Jones and Gelbart, 1993)

Comments

E(z)^+t8.8 fully rescues hemizygous lethality.

(Jones and Gelbart, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The original E(z)⁶³ chromosome may have additional mutations that mask its phenotype.

(Muller et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(Z)⁶³

(Lv et al., 2024)

E(z)63

(O'Dor et al., 2006)

E(z)⁶³

(He et al., 2023, Jangam et al., 2023, Kang et al., 2022, Leatham-Jensen et al., 2019, Dardalhon-Cuménal et al., 2018, Song et al., 2018, Kang et al., 2015, Tie et al., 2009, de Ayala Alonso et al., 2007, Parrish et al., 2007, Huang et al., 2002, Wicker-Thomas and Jallon, 2000)

E(z)^S4

(Docquier et al., 1996)

E(z)S4

(Jones and Gelbart, 1990)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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