esg05729/esg35Ce-1 pharate pupae have well-differentiated testes. The stem cells and spermatogenic cells that are normally found at the tip of the testis are missing in the mutants, with the mutant testes containing only sperm bundles and motile sperm.
The dorsal branch fusion cells form discrete branches that never meet or join. These cells go on to form additional branches that resemble normal terminal branches in the larva. Lateral trunk fusion branches are completely missing leaving a gap in each segment of the lateral trunk.
Many heteroallelic combinations studied. Where flies die as pharate adults sternite/tergites partially or completely deleted and/or legs are twisted. Where flies are viable, they have held out wings, and may have twisted legs, sternite/tergites partially deleted and be sickly. Abdominal histoblasts examined in heteroallelic combinations and found to have nuclei 3.4 fold times larger (revealed by Ecol\lacZ expression from esg05729 allele) and with 7-16 times as much DNA (DAPI fluorescence) as wild type histoblast nuclei.