FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\fandfas-1
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General Information
Symbol
Dmel\fandfas-1
Species
D. melanogaster
Name
FlyBase ID
FBal0003983
Feature type
allele
Associated gene
Dmel\fand
Associated Insertion(s)
Carried in Construct
Also Known As
fasIC
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Lekven et al., 1998)
Mutagen
ethyl methanesulfonate
(Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G?A.

(Sauerwald et al., 2017)

Predicted to result in a truncated protein lacking all of the 13 tetratricopeptide repeats of the fand protein.

(Sauerwald et al., 2017)

Amino acid replacement: W69term.

(Sauerwald et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:13,593,989..13,593,989 [+]
Nucleotide change:

G13593989A

Reported nucleotide change:

G?A

Amino acid change:

W69term | fand-PA

Reported amino acid change:

W69term

Comment:

Nonsense mutation (TGG to TAG) at Trp codon.

(Sauerwald et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      fandfas-1/fandH124 transheterozygous embryos show defective morphogenesis of the tracheal as well as the central nervous system.

      (Sauerwald et al., 2017)

      The salivary gland placodes of fas1 embryos appear identical to wild type at the gross morphological level, but the cells in the placode are stacked upon each other in two or three rows and are variably elongated (in contrast to wild type where the placode consists of a monolayer of uniformly elongated cells). At the stage when the dorsal-posterior pit forms in the salivary gland placode in wild-type embryos, a slight indentation is seen near the centre of the placode in fas1 embryos, suggestive of cell shape changes. The invaginating pit is not as deep or wide as in wild-type embryos. Cells at the centre of the pit appear elongated with basally positioned nuclei, however, the surrounding cells in the pit are round and found in multiple layers. Cells in the anterior and posterior parts of the gland also form multi-layered placodes. Although the initial indentation occurs at slightly variable locations in different embryos of similar age, the pit observed in late-stage mutant embryos is trough-shaped and uniformly located close to the centre of the placode. The salivary glands are eventually internalised, despite gross abnormalities in cell shape. The internalised gland is comprised of a mixture of elongated and wedge-shaped cells. Cells in the anterior and posterior parts of the gland are multi-layered. After internalisation, the salivary glands fuse into one dome-shaped organ which is close to the ventral surface. The gland is multi-layered and has the remnants of a potentially contiguous lumen. When internalisation is complete, the surface morphology of the salivary pit is similar to wild type.

      (Myat and Andrew, 2000)

      Malpighian tubules arrest early in their morphogenesis in homozygous embryos. The tubules are spherical. The tubules have a short lumen that is approximately normal in width. The tip cells are present. The hindgut is extremely short.

      (Jack and Myette, 1999)

      Embryos have poorly developed cuticle with necrotic patches and an abnormal head. First visible in extended-germ-band stage.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lack of primary tracheal branching characteristic for fandfas-1/fandH124 transheterozygous embryos cannot be rescued by expression of bnlScer\UAS.cSa under the control of Scer\GAL469B (which on its own causes excessive tracheal branching).

      (Sauerwald et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      fandH124

      (Sauerwald et al., 2017)

      fandP218

      (Sauerwald et al., 2017)
      Rescued by

      fandfas-1/fandH124 is rescued by fand+tGa

      (Sauerwald et al., 2017)

      fandfas-1/fandH124 is rescued by Scer\GAL4da.PU/fandUASp.Tag:MYC

      (Sauerwald et al., 2017)

      fandfas-1/fandH124 is rescued by fandUASp.Tag:MYC/Scer\GAL4Abd-B-LDN

      (Sauerwald et al., 2017)
      Partially rescued by

      fandfas-1/fandH124 is partially rescued by Scer\GAL4da.PU/fandUASp.Tag:MYC/Scer\GAL80btl

      (Sauerwald et al., 2017)
      Not rescued by

      fandfas-1/fandH124 is not rescued by Scer\GAL4btl.PS/fandUASp.Tag:MYC

      (Sauerwald et al., 2017)
      Comments

      The fandfas-1 allele fails to complement both fandP218 and fandH124 alleles.

      The tracheal branching and central nervous defects characteristic for fandfas-1/fandH124 embryos are fully rescued by combination with a single copy of fand+tGa. The lack of tracheal branching is also rescued by expression of fandScer\UAS.T:Hsap\MYC when driven by Scer\GAL4da.PU or Scer\GAL4Abd-B-LDN and partially so when driven by Scer\GAL4da.PU combined with Scer\GAL80btl (to block the expression in tracheal cells), while it cannot be rescued by Scer\GAL4btl.PS-driven expression.

      (Sauerwald et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      fandfas-1
      fas1
      (Sauerwald et al., 2017)
      fasI
      (Myat and Andrew, 2000)
      fasIC
      (Jack and Myette, 1999, Lekven et al., 1998, Tearle and Nusslein-Volhard, 1987)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)