FlyBase Allele Report: Dmel\ix[3]

General Information

Symbol

Dmel\ix³

Species

D. melanogaster

Name

FlyBase ID

FBal0005630

Feature type

allele

Associated gene

Dmel\ix

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Chase and Baker, 1995)

Mutagen

ethyl methanesulfonate

(Chase and Baker, 1995)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Siegal and Baker, 2005)

visible

(Chase and Baker, 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

Female ix³/Df(2R)en-B mutants have male-like pigmentation, especially on abdominal tergite 6. Wild-type females have one dorsal and one ventral anal plate, whereas wild-type males have one left and one right anal plate. In ix³/Df(2R)en-B females, the ventral plate develops incompletely and the dorsal plate is split such that each side is shifted laterally toward a male-like fate, although the plates commonly remain fused on the dorsal side. In ix³/Df(2R)en-B females, there is a dramatic reduction in the number of gonopod thorn bristles (also known as vaginal teeth), which form two parallel rows in either side of the vaginal opening. In ix³/Df(2R)en-B females, there is a dramatic reduction in the number of vaginal teeth, and they are non longer present in ordered rows. Bristles on the foreleg are intermediate between those found on the female and the thicker and more darkly pigmented bristles that form the sex comb of the male. The angle of the bristles is set to 45^o in ix³/Df(2R)en-B females.

(Siegal and Baker, 2005)

Diplo-X individuals carrying ix³ display an intersex phenotype. Within the range of intersexual phenotypes in homozygotes or hemizygotes both the male and female type morphological features tend to be more robust at lower temperatures and in hemizygotes. Heterozygotes with ix⁴ are intersexual at all temperatures, but the extent of intersexuality increases with increasing temperature.

(Chase and Baker, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(2R)en-B/ix³ has female sterile phenotype, suppressible by Msca\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has female sterile phenotype, suppressible by Dvir\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Other

Statement

Reference

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³ has visible | dominant phenotype

(Siegal and Baker, 2005)

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³/ix[+] has visible phenotype

(Siegal and Baker, 2005)

Df(2R)en-B/ix³, dsx[+]/dsx¹ has viable phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has viable phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx[+]/dsx⁹ has viable phenotype

(Garrett-Engele et al., 2002)

dsx¹, ix³ has viable phenotype

(Garrett-Engele et al., 2002)

dsx[+]/dsx¹, ix³ has viable phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has viable phenotype

(Garrett-Engele et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(2R)en-B/ix³ has hypogynial long bristle phenotype, suppressible by Dvir\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has hypogynial long bristle phenotype, suppressible by Msca\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has hypogynial long bristle phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Bmor\ix^UASp.cSa

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has prothoracic metatarsal segment transverse row bristle | female phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Bmor\ix^UASp.cSa

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has abdominal tergite 6 | female phenotype, suppressible by Dvir\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has abdominal tergite 6 | female phenotype, suppressible by Msca\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has cercus | female phenotype, suppressible by Scer\GAL4^αTub84B.PL/Bmor\ix^UASp.cSa

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has cercus | female phenotype, suppressible by Dvir\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has cercus | female phenotype, suppressible by Msca\ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

NOT suppressed by

Statement

Reference

Df(2R)en-B/ix³ has abdominal tergite 6 | female phenotype, non-suppressible by Scer\GAL4^αTub84B.PL/Bmor\ix^UASp.cSa

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has abdominal tergite 6 | female phenotype, non-suppressible by Mmus\Med29^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has cercus | female phenotype, non-suppressible by Mmus\Med29^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has hypogynial long bristle phenotype, non-suppressible by Mmus\Med29^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Df(2R)en-B/ix³ has prothoracic metatarsal segment transverse row bristle | female phenotype, non-suppressible by Mmus\Med29^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Other

Statement

Reference

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³ has abdominal tergite 6 | female phenotype

(Siegal and Baker, 2005)

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³ has hypogynial long bristle phenotype

(Siegal and Baker, 2005)

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³ has cercus | female phenotype

(Siegal and Baker, 2005)

Mmus\Med29^UASp.cSa, Scer\GAL4^αTub84B.PL, ix³ has prothoracic metatarsal segment transverse row bristle | female phenotype

(Siegal and Baker, 2005)

Df(2R)en-B/ix³, dsx¹ has abdominal tergite 6 | female phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has hypogynial tooth phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has abdominal tergite 6 | female phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has hypogynial tooth phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has abdominal sternite 6 phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has epiproct phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³ has prothoracic metatarsus | female phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has sex comb phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has abdominal tergite 6 | female phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has hypogynial tooth phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has sex comb phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has abdominal sternite 6 | male phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has cercus phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has epiproct phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹/dsx¹ has prothoracic metatarsus phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has abdominal sternite 6 | male phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has abdominal tergite 6 | female phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has hypogynial tooth phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has sex comb phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has cercus phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has abdominal sternite 6 | male phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has cercus phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has epiproct phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx⁹ has prothoracic metatarsus phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has epiproct phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³/ix[+] has abdominal tergite 6 | female phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³/ix[+] has hypogynial tooth phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³/ix[+] has abdominal sternite 6 phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³/ix[+] has epiproct phenotype

(Garrett-Engele et al., 2002)

dsx⁹/dsx¹, ix³/ix[+] has prothoracic metatarsus | female phenotype

(Garrett-Engele et al., 2002)

Df(2R)en-B/ix³, dsx¹ has prothoracic metatarsus phenotype

(Garrett-Engele et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

ix; dsx transheterozygous progeny from ix³/+; dsx¹/+ females crossed to Df(2R)en-B/+; dsx⁹/+

males

show little or no masculinisation of females, or feminisation of males,

as judged by: Mean number of vaginal teeth; percentage of females with

fused dorsal-lateral anal plates; percentage width of the 6th tergite

that is darkly pigmented; number of bristles in the last transverse

row in the foreleg basitarsus (note this row forms the sex comb in

males); or number of bristles on the sixth sternite.

ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutants show masculinisation

of females, as judged by the following criteria:

The mean number of vaginal teeth is reduced to 6.45 +/- 2.11. 85 %

of females have fused dorsal-lateral anal plates (as wild type; note

that even those with fused plates exhibit variable degrees of partial

fusion as judged against fusion in wild-type females). The mean percentage

width of the 6th tergite that is darkly pigmented is increased to 95%

(+/- 0.4%). The number of bristles in the last transverse row in the

foreleg basitarsus is increased to: 7.25 +/- 0.54 ( this row forms

the sex comb in males).

ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutant females do not

show significant reductions in the number of bristles on the sixth

sternite (20.75 +/- 1.83).

ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutants show feminisation

of males, as judged by the following criteria:

An average of 6.85 (+/- 2.74) ectopic vaginal teeth form.

Fusion of dorso-lateral anal plates is seen in 80% of males.

The number of bristles in the last transverse row in the foreleg basitarsus

is decreased to 7.25 +/-0.54 (this row forms the sex comb in males).

The average number of bristles on the sixth sternite increases dramatically

from near zero to 18.2 +/- 1.58.

ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutant males do not

show significant masculinisation of pigmentation of the 6th tergite,

which remains

96% (+/-3%) darkly pigmented.

ix³/Df(2R)en-B mutant females heterozygous for dsx⁹ or dsx¹

show masculinisation, as judged by the following criteria:

Mean number of vaginal teeth is reduced to 9.7 +/- 5.29. 90 % of females

have fused dorsal-lateral anal plates (as wild type; note that even

those with fused plates exhibit variable degrees of partial fusion

as judged against fusion in wild-type females). The mean percentage

width of the 6th tergite that is darkly pigmented is increased to 92%

(+/-6%). The number of bristles in the last transverse row in the foreleg

basitarsus is increased to: 6.88 +/- 0.65 (this row forms the sex comb

in males).

ix³/Df(2R)en-B mutant females transheterozygous for dsx⁹/+

or dsx¹/+ do not show significant reductions in the number of bristles

on the sixth sternite (21.1 +/-1.68)

ix³/Df(2R)en-B mutant males heterozygous for dsx⁹ or dsx¹

do not show feminisation, as judged by the following criteria:

Mean number of vaginal teeth; percentage of males with fused dorsal-lateral

anal plates; percentage width of the 6th tergite that is darkly pigmented;

number of bristles in the last transverse row in the foreleg basitarsus

(note this row forms the sex comb in males); or number of bristles

on the sixth sternite.

dsx⁹/dsx¹ mutant females heterozygous for ix³ or Df(2R)en-B

show masculinisation, as judged by the following criteria:

Mean number of vaginal teeth is reduced to 6.0 +/-3.55. 65 % of females

have fused dorsal-lateral anal plates (as wild type; note that even

those with fused plates exhibit variable degrees of partial fusion

as judged against fusion in wild-type females). The mean percentage

width of the 6th tergite that is darkly pigmented is increased to 96%

(+/-5%). The number of bristles in the last transverse row in the foreleg

basitarsus is increased to: 7.75 +/- 0.78 (this row forms the sex comb

in males). The average number of bristles on the sixth sternite is

slightly decreased to 19.5 +/- 1.91.

dsx⁹/dsx¹ mutant males heterozygous for ix³ or Df(2R)en-B

show feminisation, as judged by the following criteria:

An average of 6 (+/- 4.19) ectopic vaginal teeth form. Fusion of dorso-lateral

anal plates is seen in 70% of males. The number of bristles in the

last transverse row in the foreleg basitarsus is decreased to 7.45

+/-0.6 (this row forms the sex comb in males). The average number

of bristles on the sixth sternite increases dramatically from near

zero to 17.3 +/- 2.06.

dsx⁹/dsx¹ mutant males heterozygous for ix³ or Df(2R)en-B

do not show significant masculinisation of pigmentation of the 6th

tergite, which remains 95% (+/-4%) darkly pigmented.

96% (+/-3%) darkly pigmented.

(Garrett-Engele et al., 2002)

Xenogenetic Interactions

Statement

Reference

Expressing Dvir\ix^{Scer\UAS.P\T.cSa}, under the control of Scer\GAL4^αTub84B.PL fully suppresses the ix³/Df(2R)en-B phenotype. Expressing Msca\ix^{Scer\UAS.P\T.cSa}, under the control of Scer\GAL4^αTub84B.PL fully suppresses the ix³/Df(2R)en-B phenotype. Expressing Bmor\ix^{Scer\UAS.P\T.cSa}, under the control of Scer\GAL4^αTub84B.PL does not fully suppress the ix³/Df(2R)en-B phenotype. Moreover, wild-type female development is not fully restored when two copies of Bmor\ix^{Scer\UAS.P\T.cSa} are present. These females also manifest some morphological defects. Pigmentation of abdominal tergite 6 is male-like. The dorsal and ventral anal plates, however, are present and appear normal. The vaginal teeth are present in two neat rows but there are gaps where bristles would be expected to be present. Likewise the last transverse row bristles, which form the sex combs in the male, appear mostly female-like, but are slightly darker and rotated upward. Expressing Mmus\Ixl^{Scer\UAS.P\T.cSa} in the fly, under the control of Scer\GAL4^αTub84B.PL does not rescue the ix³/Df(2R)en-B phenotype. The pigmentation of abdominal tergite 6 was male-like, the anal plates were shifted laterally, the vaginal teeth were reduced in number and not present in rows and the last transverse row bristles, which form the sex combs in the male, were darker, thicker, and rotated upwards, as in the male. Expressing Mmus\Ixl^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in heterozygous ix³ females yields a strong intersexual transformation. Indeed females heterozygous for endogenous ix and expressing Mmus\Ixl^{Scer\UAS.P\T.cSa} are indistinguishable from females homozygous ix³ mutant and expressing Mmus\Ixl^{Scer\UAS.P\T.cSa}, suggesting that Mmus\Ixl^{Scer\UAS.P\T.cSa} acts in a dominant-negative manner.

(Siegal and Baker, 2005)

Complementation and Rescue Data

Rescued by

ix³ is rescued by ix^UASp.cSa/Scer\GAL4^αTub84B.PL

(Siegal and Baker, 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66705

w^*; ix³/CyO; Ki¹/TM6B, Tb¹

Notes on Origin

Discoverer

Duncan.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

D10036a

(Taylor, 1992)

ix³

(Sanders and Arbeitman, 2008)

ix^D100.36

(Lee and Hall, 2000)

Name Synonyms

Secondary FlyBase IDs

References (7)

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