FlyBase Allele Report: Dmel\lmgA[03424]

General Information

Symbol

Dmel\lmgA⁰³⁴²⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0008025

Feature type

allele

Associated gene

Dmel\lmgA

Associated Insertion(s)

P{PZ}lmgA⁰³⁴²⁴

Carried in Construct

Also Known As

lmg⁰³⁴²⁴

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Taylor et al., 1997, Taylor and Shirras, 1995)

Progenitor genotype

Associated Insertion(s)

P{PZ}lmgA⁰³⁴²⁴

Cytology

Description

P{PZ} insertion in the 5' untranslated region.

(Shirras, 2000.1.5)

The P{PZ}lmg⁰³⁴²⁴ insertion maps within a dicistronic processed transcript that encodes two non-overlapping open reading frames (lmg and CG34441).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,528,450..8,528,450 [+]

(Nagy et al., 2012, Kuo et al., 2006, Watts et al., 2003, Shirras, 2000.1.5, Spradling et al., 1999, Taylor et al., 1997, FlyBase, 1996-, Taylor and Shirras, 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Taylor and Shirras, 1995)

histoblast

(Taylor and Shirras, 1995)

larval brain | third instar larval stage

(Nagy et al., 2012)

neuroblast | somatic clone

(Watts et al., 2003)

wing disc | third instar larval stage

(Nagy et al., 2012)

Detailed Description

Statement

Reference

lmgA⁰³⁴²⁴/lmgA⁰³⁴²⁴ embryos have no muscle defects.

(Drechsler et al., 2018)

Homozygotes die at pharate adult stage P15(i). The mitotic index in third larval instar brains is increased compared to wild type and 19.6 +/- 5.6 % of the cells are polyploid. Third larval instar brains and wing imaginal discs show increased levels of apoptosis compared to wild type.

(Nagy et al., 2012)

lmg⁰³⁴²⁴ mutant C4da neuronal clones do not exhibit defects in dendrite pruning or neuronal cell death 18-20 hours after puparium formation.

(Kuo et al., 2006)

No defects are seen in axon pruning during metamorphosis, though severe proliferation defects ar seen when somatic clones are made in the neuroblast.

(Watts et al., 2003)

Reduced eye. Lack of adult abdominal cuticle due to apoptosis of histoblast cells.

(Taylor and Shirras, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

lmg[+]/lmgA⁰³⁴²⁴ is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

lmgA⁰³⁴²⁴, mr² has abnormal mitotic cell cycle | third instar larval stage phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, mr² has lethal | prepupal stage P4(ii) phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, mr² has increased occurrence of cell division | third instar larval stage phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, vih^S110501 has abnormal mitotic cell cycle | third instar larval stage phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, vih^S110501 has lethal | prepupal stage P3 phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, vih^S110501 has increased occurrence of cell division | third instar larval stage phenotype

(Nagy et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

lmg[+]/lmgA⁰³⁴²⁴ is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

lmg[+]/lmgA⁰³⁴²⁴ is a suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

fzr^ie28, lmgA⁰³⁴²⁴ has embryonic somatic muscle cell phenotype

(Drechsler et al., 2018)

fzr^ie28, lmgA⁰³⁴²⁴ has embryonic myoblast | late embryonic stage phenotype

(Drechsler et al., 2018)

fzr^ie28, lmgA[+]/lmgA⁰³⁴²⁴ has embryonic somatic muscle cell phenotype

(Drechsler et al., 2018)

fzr^ie28, lmgA[+]/lmgA⁰³⁴²⁴ has embryonic myoblast | late embryonic stage phenotype

(Drechsler et al., 2018)

lmgA⁰³⁴²⁴, mr² has larval brain | third instar larval stage phenotype

(Nagy et al., 2012)

lmgA⁰³⁴²⁴, vih^S110501 has larval brain | third instar larval stage phenotype

(Nagy et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

fzr^ie28/+ ; lmgA⁰³⁴²⁴/+ embryos show disrupted myoblast fusion but an overall wild-type muscle morphology, while myoblast fusion is completely abolished in fzr^ie28/+ ; lmgA⁰³⁴²⁴/lmgA⁰³⁴²⁴ embryos at stages 16-17.

(Drechsler et al., 2018)

lmgA⁰³⁴²⁴ mr² double mutants die at prepupal stage P4(ii). The mitotic index in third larval instar brains is increased compared to wild type and 31.8 +/- 3.9 % of the cells are polyploid. Third larval instar brains and wing imaginal discs show increased levels of apoptosis compared to wild type.

lmgA⁰³⁴²⁴ vih^S110501 double mutants die at bubble prepupa stage P3. The mitotic index in third larval instar brains is increased compared to wild type and 44.3 +/- 8.4 % of the cells are polyploid. Third larval instar brains and wing imaginal discs show increased levels of apoptosis compared to wild type.

(Nagy et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11330

P{PZ}lmgA⁰³⁴²⁴ lmgB⁰³⁴²⁴ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(2)01482⁰¹⁴⁸². Complements: Sema-1a^k03509. Complements: l(2)k05125a^k05125a. Complements: Acer^k07704. Complements: Sema-1a^k13702. Complements: l(2)k15306^k15306. Complements: l(2)rH280^rH280.

(BDGP Project Members, 1994-1999)

Imprecise excision of the P{PZ} element by Δ2-3 transposase generates null mutations.

(Taylor and Shirras, 1995)

Precise excision of the insertion reverts the mutant phenotype.

(Nagy et al., 2012)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

03424

(Watts et al., 2003)

Orct⁰³⁴²⁴

l(2)03424

(Shirras, 2000.1.5, Spradling et al., 1999, Taylor and Shirras, 1997, Taylor and Shirras, 1995)

l(2)03424⁰³⁴²⁴

lmg⁰³⁴²⁴

(Drechsler et al., 2018, Neuert et al., 2017, Nagy et al., 2012, Tanaka-Matakatsu et al., 2007, Christensen and Cook, 2006.8.30)

lmgA⁰³⁴²⁴

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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