Mitochondria in muscle fibres 6/7 in abdominal segments of tam3/tam9 mutant third instar larvae exhibit decreased mtDNA content, increased mitochondrial density and mitochondria appear abnormally tightly packed instead of arranged in normal banded patterns, but there is no qualitative difference in mitochondrial morphology when examined via electron microscopy, as compared to controls. Third instar larvae display significantly higher mitochondrial density in the proximal region of the segmental nerve, but not in the medial or distal regions. Mitochondrial flux in tam3/tam9 third instar motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity, but a significant decrease in anterograde velocity, as compared to controls.
There is no significant difference in synaptic vesicle precursor flux or velocity in tam3/tam9 or tam3/+ third instar motor neurons, as compared to controls.
Mitochondrial flux in third instar tam3/+ motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity of mitochondria, but there is a significant decrease in anterograde velocity, as compared to controls.
Hemizygous lethality occurs during the late third larval instar stage. tam3/tam9 larvae fail to wander at the appropriate time. tam9/tam3 larvae have smaller eye discs than normal and show delayed photoreceptor differentiation. The area of differentiated photoreceptors is abnormal and their projection pattern is abnormal. tam3/tam9 larvae move significantly less than wild-type larvae.