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General Information
Symbol
Dmel\PolG13
Species
D. melanogaster
Name
FlyBase ID
FBal0008416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tam3
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Mutation is a 5bp deletion just after conserved DNA polymerase domain Z and results in a frameshift of the rest of the protein.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

5bp deletion (of nucleotides 3371 to 3375). Deletion is in exon 3 just after conserved DNA polymerase domain Z and results in a frameshift of the rest of the protein.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mitochondria in muscle fibres 6/7 in abdominal segments of tam3/tam9 mutant third instar larvae exhibit decreased mtDNA content, increased mitochondrial density and mitochondria appear abnormally tightly packed instead of arranged in normal banded patterns, but there is no qualitative difference in mitochondrial morphology when examined via electron microscopy, as compared to controls. Third instar larvae display significantly higher mitochondrial density in the proximal region of the segmental nerve, but not in the medial or distal regions. Mitochondrial flux in tam3/tam9 third instar motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity, but a significant decrease in anterograde velocity, as compared to controls.

There is no significant difference in synaptic vesicle precursor flux or velocity in tam3/tam9 or tam3/+ third instar motor neurons, as compared to controls.

Mitochondrial flux in third instar tam3/+ motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity of mitochondria, but there is a significant decrease in anterograde velocity, as compared to controls.

Hemizygous lethality occurs during the late third larval instar stage. tam3/tam9 larvae fail to wander at the appropriate time. tam9/tam3 larvae have smaller eye discs than normal and show delayed photoreceptor differentiation. The area of differentiated photoreceptors is abnormal and their projection pattern is abnormal. tam3/tam9 larvae move significantly less than wild-type larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially complements
Comments

tamRescue complements tam3 to produce viable flies.

Homozygous tam9 larvae show no response to light in contrast to wild-type larvae. Larvae transheterozygous for tam9 and tam3 respond to light, suggesting partial complementation of this phenotype.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer

Ashburner.

Separable from: a second-site mutation. Probably associated with a second-site mutation, since homozygotes die during late embryonic stages whereas hemizygotes die during the late third larval instar stage.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)