FB2026_02 , released June 18, 2026
Allele: Dmel\PolG12
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General Information
Symbol
Dmel\PolG12
Species
D. melanogaster
Name
FlyBase ID
FBal0008415
Feature type
allele
Associated gene
Dmel\PolG1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ashburner et al., 1999.10.12, Woodruff and Ashburner, 1979)
Progenitor genotype
Cytology
Description

Amino acid replacement: E813V. Mutation in a highly conserved polymerase domain.

(Iyengar et al., 2001)

Nucleotide substitution: A2440T. Amino acid replacement: E813V. Mutation is in polymerase domain X.

(Iyengar et al., 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:13,825,199..13,825,199 [-]
Nucleotide change:

A13825199T

Reported nucleotide change:

A2440T

Amino acid change:

E813V | PolG1-PA

Reported amino acid change:

E813V

(Iyengar et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The reticular pattern of mitochondria surrounding the nuclei of larval brain cells is disrupted in tam2/Df(2L)b80c1 animals. The CNS of tam2/Df(2L)b80c1 larvae is smaller than that of wild-type controls. These larvae have a reduction in cell proliferation (measured by BrdU incorporation) in the CNS. Both the outer and inner proliferation centers in the optic lobes are equally affected.

      (Iyengar et al., 2002)

      Hemizygous lethality occurs during the late third larval instar stage.

      (Iyengar et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      DNApol-γ352; tam2 and DNApol-γ351; tam2 double heterozygotes are viable.

      (Iyengar et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially complements

      PolG110

      (Iyengar et al., 1999)
      Fails to complement

      PolG19

      (Iyengar et al., 1999)

      PolG14

      (Iyengar et al., 1999)

      PolG13

      (Iyengar et al., 1999)
      Comments

      Homozygous tam9 larvae show no response to light in contrast to wild-type larvae. Larvae transheterozygous for tam9 and tam2 respond to light, suggesting partial complementation of this phenotype.

      (Iyengar et al., 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Separable from: a second-site mutation. Probably associated with a second-site mutation, since homozygotes die during early larval stages whereas hemizygotes die during the late third larval instar stage.

      (Iyengar et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      PolG12
      SF22
      (Woodruff and Ashburner, 1979)
      l(2)34Dc2
      (Ashburner et al., 1999.10.12)
      l(2)br5SF22
      (Woodruff and Ashburner, 1979)
      l(2)brSSF22
      (The Moscow Regional Drosophila melanogaster Stock Center, Dubna)
      tam2
      tamas2
      Name Synonyms
      Secondary FlyBase IDs
        References (6)