FlyBase Allele Report: Dmel\Mbs[3]

General Information

Symbol

Dmel\Mbs³

Species

D. melanogaster

Name

FlyBase ID

FBal0009950

Feature type

allele

Associated gene

Dmel\Mbs

Associated Insertion(s)

Carried in Construct

Also Known As

DMBS^E1, l(3)72Dd³

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | dorsal, with Mbs^EP3727/Df(3L)th117

(Mizuno et al., 2002)

embryonic/first instar larval cuticle | dorsal (with Df(3L)th117), with Mbs^EP3727

(Mizuno et al., 2002)

embryonic leading edge cell, with Mbs^EP3727/Df(3L)th117

(Mizuno et al., 2002)

embryonic leading edge cell (with Df(3L)th117), with Mbs^EP3727

(Mizuno et al., 2002)

imaginal disc | third instar larval stage (with Mbs^P2r31)

(Mitonaka et al., 2007)

larval dorsal hair, with Mbs^EP3727/Df(3L)th117

(Mizuno et al., 2002)

larval dorsal hair (with Df(3L)th117), with Mbs^EP3727

(Mizuno et al., 2002)

larval neuroblast | third instar larval stage (with Df(3L)Exel6127)

(Tsankova et al., 2017)

wing disc | third instar larval stage (with Mbs^P2r31)

(Mitonaka et al., 2007)

Detailed Description

Statement

Reference

Mbs³/Df(3L)Exel6127 mutant third instar larval neuroblasts display abnormalities in cell shape changes from early anaphase to telophase during asymmetric cell division: the furrowing is initiated in extreme basal positions relative to wild-type neuroblast but subsequently shifts apically, past the normal wild-type furrow position, causing a slight reduction in the physical asymmetry of the cell division.

(Tsankova et al., 2017)

Homozygotes for Mbs³ are lethal during early larval stages.

Transheterozygous Mbs³/Mbs^P2r31 animals grow slowly. They become wandering third instar larvae 6 to 7 days after egg laying (AEL) as compared with the normal 5 days AEL. After starting to wander, the mutant larvae are semi-paralysed, and survive for several days without forming puparium. In such mutant larvae, imaginal discs continue to grow and develop to a slightly larger size than that of wild-type discs. These mutant imaginal discs, compared with wild-type, are disorganised and multi-layered. Furthermore, adjacent imaginal discs are frequently fused to each other, suggesting a tumorous growth of imaginal cells.

In the wild-type wing imaginal disc, marked cells are observed within a straight stripe along the anterior-posterior boundary. In contrast, the anterior-posterior boundary in Mbs³/Mbs^P2r31 mutant discs, as visualised by marked cell clones, appears diffuse, not well defined.

(Mitonaka et al., 2007)

Embryonic development in embryos derived from Mbs^EP3727/Df(3L)th117 females mated to wild-type males is normal in most cases. However, about 25% of embryos derived from Mbs^EP3727/Df(3L)th117 females mated to Mbs³/+ males fail to hatch. About 80% of the embryos that fail to hatch have a "dorsal open" phenotype, and in the remaining embryos the pattern of dorsal hairs is disturbed along the dorsal midline. The leading edge cells fail to fully elongate during dorsal closure, with some remaining polygonal, in Mbs mutant embryos derived from Mbs^EP3727/Df(3L)th117 females mated to Mbs³/+ males. The epidermal cells located more ventrally elongate nearly normally. There is an abnormal accumulation of F-actin within the leading edge cells, while its distribution along the leading edge is essentially unaffected.

(Mizuno et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mbs³/Df(3L)th117, Mbs^EP3727 has lethal | maternal effect | embryonic stage phenotype, suppressible by zip[+]/zip^Ebr

(Mizuno et al., 2002)

Mbs^EP3727/Df(3L)th117, Mbs³ has lethal | maternal effect | embryonic stage phenotype, suppressible by zip[+]/zip^Ebr

(Mizuno et al., 2002)

NOT Enhancer of

Statement

Reference

Mbs³ is a non-enhancer of viable phenotype of flw¹

(Vereshchagina et al., 2004)

Suppressor of

Statement

Reference

Mbs³ is a suppressor of visible | dominant phenotype of Rho1^72BH, zip^Ebr

(Mizuno et al., 2002)

Mbs³/Mbs[+] is a suppressor | partially of visible phenotype of zip⁰²⁹⁵⁷/zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor of visible | dominant phenotype of Rok², zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor of visible | dominant phenotype of RhoGEF2⁰⁴²⁹¹, zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor | partially of visible | dominant phenotype of Rho1^72O, zip^Ebr

(Mizuno et al., 2002)

Other

Statement

Reference

Mbs³/Mbs[+], flw¹, puc^A251.1F3/puc[+] has lethal phenotype

(Kirchner et al., 2007)

Mbs³/Mbs[+], flw¹ has viable phenotype

(Kirchner et al., 2007)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mbs³ is a suppressor of wing phenotype of Rho1^72BH, zip^Ebr

(Mizuno et al., 2002)

Mbs³/Mbs[+] is a suppressor | partially of wing phenotype of zip⁰²⁹⁵⁷/zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor of wing phenotype of Rok², zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor of wing phenotype of RhoGEF2⁰⁴²⁹¹, zip^Ebr

(Mizuno et al., 2002)

Mbs³ is a suppressor | partially of wing phenotype of Rho1^72O, zip^Ebr

(Mizuno et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The Mbs³ mutation does not affect the viability of flw¹ mutants.

(Vereshchagina et al., 2004)

The lethality of Mbs mutant embryos derived from Mbs^EP3727/Df(3L)th117 females mated to Mbs³/+ males is suppressed by zip^Ebr/+.

(Mizuno et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Mbs^EP3727

(Mizuno et al., 2002)

Partially rescued by

Mbs³ is partially rescued by Mbs^L.hs

(Mizuno et al., 2002)

Mbs³ is partially rescued by Mbs^S.hs

(Mizuno et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

5054

108012

Notes on Origin

Discoverer

Brizuela.

Comments

Strength of phenotype: Mbs³ = Mbs⁰³⁸⁰² > Mbs^P2r31 > Mbs^EP3727.

(Mizuno et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DMBS^E1

(Mitonaka et al., 2007, Vereshchagina et al., 2004, Mizuno et al., 2002)

Mbs³

(Zeledon et al., 2019, Christensen et al., 2009.5.6, Christensen et al., 2008.4.15, Kirchner et al., 2007)

l(3)72Dd³

(Mitonaka et al., 2007, Mizuno et al., 2002)

mbs³

(Tsankova et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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