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General Information
Symbol
Dmel\Vha5514
Species
D. melanogaster
Name
FlyBase ID
FBal0010428
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    G12625418A

    Reported nucleotide change:

    G?A

    Amino acid change:

    E295K | Vha55-PB; E295K | Vha55-PA; E295K | Vha55-PC

    Reported amino acid change:

    E?K

    Comment:

    Reported as a missense mutation in the second coding exon of Vha55 in the Glu of the string AREEV from GAG to AAG. Also carries a T to C mutation in an upstream intron.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    T to C nucleotide substitution within an intron, plus non-conservative change of Glu to Lys.

    Point mutation.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Vha5514 homozygous flies expressing Vha55Scer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PU (rescue flies) are viable and their Malphigian tubules show normal resting secretion rates but reduced maximum stimulation fluid secretion.

    Mutants sometimes have clear Malpighian tubules (when examined by birefringence).

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference

    Vha55[+]/Vha5514 is an enhancer of visible | heat sensitive phenotype of Ivir\M2UAS.cAa, Scer\GAL4GMR.PU

    NOT Suppressor of
    Statement
    Reference
    Other
    Statement
    Reference
    Phenotype Manifest In
    Enhancer of
    Statement
    Reference

    Vha55[+]/Vha5514 is an enhancer of eye | heat sensitive phenotype of Ivir\M2UAS.cAa, Scer\GAL4GMR.PU

    NOT Suppressor of
    Statement
    Reference
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Vha5514 corto420 double heterozygotes show posterior crossvein defects.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    The lethality of Vha5514 homozygous mutants is rescued by expression of Vha55Scer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PU in the mutant background.

    Images (0)
    Mutant
    Wild-type
    Stocks (2)
    Notes on Origin
    Discoverer

    Gelbart.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (8)