Vacuolar H+-ATPase 55kD B subunit, l(3)87Ca, l(3)j2E9, SzA, V-ATPase
Gene model reviewed during 5.50
Low-frequency RNA-Seq exon junction(s) not annotated.
2.8, 2.6, 2.5, 2.0, 1.8 (longest cDNA)
490 (aa); 55 (kD)
V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vha55 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Vha55 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against Vha55 that is transfected into S2 treated with Listeria monocytogenes reveals Vha55 to be involved in Listeria monocytogenes entry and vacuolar escape.
Vha55 is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Alleles of Pc, Pcl, Scm, Dll, brm, kto, Scr and trx show clear dominant enhancement or suppression of AntpScx, whereas alleles of vtd, Vha55, Su(Pc)37D, urd, mor, skd and osa do not.
Most extreme hemizygous phenotype is embryonic lethality, even though homozygous Df(3R)kar3J, which lacks the locus entirely, dies during the first larval instar (Ish-Horowicz, Holden and Gehring, 1977). First instar larvae have transparent Malpighian tubules; autonomous in transplants. Two alleles isolated on the basis of their dominant suppression of ectopic sex-comb teeth in Pc4/+ males (Kennison and Tamkun, 1988).