Flies homozygous mutant for any of the following: Vha557e1, Vha5514 and Vha559, which are also expressing Vha55Scer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PU (rescue flies) are viable and their Malphigian tubules show normal resting secretion rates but reduced maximum stimulation fluid secretion.
Vha55UAS.GFP/Scer\GAL4hs.PU rescues Vha559
Vha55UAS.GFP/Scer\GAL4hs.PU rescues Vha5514
The lethality of each of the following homozygous mutants: Vha557e1, Vha5514 and Vha559 is rescued by expression of Vha55Scer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PU in the mutant background.