FB2025_01 , released February 20, 2025
Reference Report
Open Close
Reference
Citation
Sun, Y., An, S., Henrich, V.C., Sun, X., Song, Q. (2007). Proteomic Identification of PKC-Mediated Expression of 20E-Induced Protein in Drosophila melanogaster.  J. Proteome Res. 6(11): 4478--4488.
FlyBase ID
FBrf0201893
Publication Type
Research paper
Abstract
Ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle protein (USP), are nuclear receptors that mediate the action of the insect molting hormone 20-hydroxyecdysone (20E). There is evidence that the activity of both receptors is affected by phosphorylation. Using a proteomic approach, we have shown that protein kinase C (PKC) activity is necessary for mediating 20E-induced expression of 14 specific proteins, including three previously reported 20E responsive proteins, and is also responsible for the intracellular localization of EcR and USP in larval salivary glands of Drosophila melanogaster. The 20E-dependent expression of the proteins was verified using real-time PCR and/or Western blot analysis. For some genes, inhibition of PKC activity reduced 20E-dependent transcriptional activity rapidly, raising the possibility that these are direct gene targets of EcR and USP. The data further indicate that PKC-mediated phosphorylation is also required for genes regulated indirectly by 20E-induced changes in the larval salivary gland.
PubMed ID
PubMed Central ID
Related Publication(s)
Erratum

Proteomic Identification of PKC-Mediated Expression of 20E-Induced Protein in Drosophila melanogaster.
Sun et al., 2008, J. Proteome Res. 7(4): 1784 [FBrf0205165]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Proteome Res.
    Title
    Journal of Proteome Research
    Publication Year
    2002
    ISBN/ISSN
    1535-3893
    Data From Reference
    Genes (16)