FlyBase Allele Report: Dmel\mrn[3]

General Information

Symbol

Dmel\mrn³

Species

D. melanogaster

Name

FlyBase ID

FBal0012436

Feature type

allele

Associated gene

Dmel\mrn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bloomington Drosophila Stock Center, 1999.6.23)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C673T.

(Fregoso et al., 2007)

Amino acid replacement: Q225term.

(Fregoso et al., 2007, Matsuno et al., 2007)

Amino acid replacement: Q225@.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:15,579,779..15,579,779 [-]

Nucleotide change:

C15579779T

Reported nucleotide change:

C673T

Amino acid change:

Q225term | mrn-PA

Reported amino acid change:

Q225term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Fregoso et al., 2007, Matsuno et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage (with mrn^EP3605)

(Fregoso et al., 2007)

decreased body size | larval stage

(Fregoso et al., 2007)

decreased body size | pupal stage

(Fregoso et al., 2007)

lethal

(Matsuno et al., 2007)

lethal (with Df(3L)ED218)

(Miller et al., 2020)

lethal (with mrn¹)

(Fregoso et al., 2007)

lethal (with mrn⁵)

(Fregoso et al., 2007)

lethal (with mrn^EP572)

(Fregoso et al., 2007)

lethal | recessive

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007)

melanotic mass phenotype | larval stage (with mrn¹)

(Fregoso et al., 2007)

melanotic mass phenotype | larval stage (with mrn^EP3605)

(Fregoso et al., 2007)

partially lethal - majority die (with mrn^EP3605)

(Fregoso et al., 2007)

radiation sensitive | dominant

(Fregoso et al., 2007)

sterile (with mrn^EP3605)

(Fregoso et al., 2007)

Phenotype Manifest In

egg chamber (with mrn^EP3605)

(Fregoso et al., 2007)

larval hindgut imaginal ring (with mrn^EP3605)

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn¹)

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn^EP3605)

(Fregoso et al., 2007)

nurse cell (with mrn^EP3605)

(Fregoso et al., 2007)

Detailed Description

Statement

Reference

11.6% of mrn¹/mrn³ larvae have necrosis or melanotic tumours.

14% of mrn^EP3605/mrn³ larvae have melanotic tumours. In addition, there is a highly reproducible and specific melanisation at the intersection between the midgut and posterior gut, in the imaginal ring region.

Heterozygous larvae are more sensitive to UVB irradiation compared to controls.

Homozygous larvae and pupae are smaller than controls. mrn^EP3605/mrn³ adult escapers are smaller than controls.

Aberrant egg chambers are seen in mrn^EP3605/mrn³ females and pycnotic nuclei are seen in nurse cells.

(Fregoso et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mrn^EP3605/mrn³ has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn³ has lethal | recessive phenotype, suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn³ has lethal | recessive phenotype, suppressible | partially by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

mrn^EP3605/mrn³ has sterile phenotype, suppressible by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Tfb5^Hsp83.PAF can suppress the lethality of mrn³ homozygotes; mrn³ flies carrying one or two copies of Tfb5^Hsp83.PAF show low, but significant, rescue of lethality, while mrn³ flies carrying three copies of Tfb5^Hsp83.PAF are viable and fertile.

(Aguilar-Fuentes et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

mrn^EP3605

(Fregoso et al., 2007)

Fails to complement

mrn^EP572

(Fregoso et al., 2007)

mrn⁵