FlyBase Allele Report: Dmel\mrn[5]

General Information

Symbol

Dmel\mrn⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0012438

Feature type

allele

Associated gene

Dmel\mrn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bloomington Drosophila Stock Center, 1999.6.23)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C286T.

(Fregoso et al., 2007)

Amino acid replacement: Q96term.

(Fregoso et al., 2007, Matsuno et al., 2007)

Amino acid replacement: Q96@.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:15,580,221..15,580,221 [-]

Nucleotide change:

C15580221T

Reported nucleotide change:

C286T

Amino acid change:

Q96term | mrn-PA

Reported amino acid change:

Q96term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Fregoso et al., 2007, Matsuno et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with mrn^EP3605)

(Fregoso et al., 2007)

decreased body size | larval stage

(Fregoso et al., 2007)

decreased body size | pupal stage

(Fregoso et al., 2007)

lethal

(Matsuno et al., 2007)

lethal (with mrn¹)

(Fregoso et al., 2007)

lethal (with mrn³)

(Fregoso et al., 2007)

lethal (with mrn^EP572)

(Fregoso et al., 2007)

lethal | recessive

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007)

lethal - all die before end of larval stage | recessive

melanotic mass phenotype | larval stage (with mrn^EP3605)

(Fregoso et al., 2007)

partially lethal (with mrn^EP3605)

(Fregoso et al., 2007)

radiation sensitive | dominant

(Fregoso et al., 2007)

some die during larval stage | recessive

sterile (with mrn^EP3605)

(Fregoso et al., 2007)

visible (with mrn^EP3605)

(Aguilar-Fuentes et al., 2008)

Phenotype Manifest In

adult abdomen (with mrn^EP3605)

(Aguilar-Fuentes et al., 2008)

adult thorax (with mrn^EP3605)

(Aguilar-Fuentes et al., 2008)

chaeta (with mrn^EP3605)

(Aguilar-Fuentes et al., 2008)

condensed chromosome (with mrn^EP3605)

(Fregoso et al., 2007)

larval hindgut imaginal ring (with mrn^EP3605)

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn^EP3605)

(Fregoso et al., 2007)

Detailed Description

Statement

Reference

mrn⁵/mrn^EP3605 adults have defective bristles and a thin thorax and abdomen (100% penetrance).

(Aguilar-Fuentes et al., 2008)

88% of mrn^EP3605/mrn⁵ larvae have melanotic tumours. In addition, there is a highly reproducible and specific melanisation at the intersection between the midgut and posterior gut, in the imaginal ring region.

Heterozygous larvae are more sensitive to UVB irradiation compared to controls.

Homozygous larvae and pupae are smaller than controls.

Mitotic spreads of mrn^EP3605/mrn⁵ larval neuroblasts show a high rate of chromosome aberrations, including fragmentation of chromosomes and partial chromosome condensation.

(Fregoso et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mrn⁵/mrn^EP3605 has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn⁵/mrn^EP3605 has visible phenotype, suppressible by Tfb5^Hsp83.PAF