FlyBase Allele Report: Dmel\mrn[EP3605]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\mrn^EP3605

General Information

Symbol

Dmel\mrn^EP3605

Species

D. melanogaster

Name

FlyBase ID

FBal0155762

Feature type

allele

Associated gene

Associated Insertion(s)

P{EP}mrn^EP3605

Carried in Construct

Also Known As

EP3605

Key Links

Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Fregoso et al., 2007)

Mutagen

P-element activity

(FlyBase, 1996-)

Progenitor genotype

Associated Insertion(s)

P{EP}mrn^EP3605

Cytology

Description

Insertion 20 nucleotides upstream of the predicted translation initiation codon.

(Fregoso et al., 2007)

Allele components

Component

Use(s)

Inserted element

misexpression element

Regulatory region(s)

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:15,580,524..15,580,524 [+]

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007, Matsuno et al., 2007, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with mrn¹)

(Fregoso et al., 2007)

abnormal mitotic cell cycle (with mrn⁵)

(Fregoso et al., 2007)

decreased body size | adult stage (with mrn³)

(Fregoso et al., 2007)

decreased body size | pupal stage

(Fregoso et al., 2007)

lethal (with mrn^EP572)

(Fregoso et al., 2007)

melanotic mass phenotype | larval stage (with mrn³)

(Fregoso et al., 2007)

melanotic mass phenotype | larval stage (with mrn⁵)

(Fregoso et al., 2007)

partially lethal (with mrn⁵)

(Fregoso et al., 2007)

partially lethal - majority die (with mrn¹)

(Fregoso et al., 2007)

partially lethal - majority die (with mrn³)

(Fregoso et al., 2007)

radiation sensitive | dominant

(Fregoso et al., 2007)

(Fregoso et al., 2007)

sterile (with mrn¹)

(Fregoso et al., 2007)

sterile (with mrn³)

(Fregoso et al., 2007)

sterile (with mrn⁵)

(Fregoso et al., 2007)

(Fregoso et al., 2007)

visible (with mrn⁵)

(Aguilar-Fuentes et al., 2008)

Phenotype Manifest In

adult abdomen (with mrn⁵)

(Aguilar-Fuentes et al., 2008)

adult thorax (with mrn⁵)

(Aguilar-Fuentes et al., 2008)

chaeta (with mrn⁵)

(Aguilar-Fuentes et al., 2008)

chaeta | pharate adult stage

(Fregoso et al., 2007)

condensed chromosome (with mrn¹)

(Fregoso et al., 2007)

condensed chromosome (with mrn⁵)

(Fregoso et al., 2007)

cuticle | pharate adult stage

(Fregoso et al., 2007)

egg chamber (with mrn¹)

(Fregoso et al., 2007)

egg chamber (with mrn³)

(Fregoso et al., 2007)

(Fregoso et al., 2007)

larval hindgut imaginal ring (with mrn³)

(Fregoso et al., 2007)

larval hindgut imaginal ring (with mrn⁵)

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn³)

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn⁵)

(Fregoso et al., 2007)

nurse cell (with mrn¹)

(Fregoso et al., 2007)

nurse cell (with mrn³)

(Fregoso et al., 2007)

testis (with mrn¹)

(Fregoso et al., 2007)

Detailed Description

Statement

Reference

14% of mrn^EP3605/mrn³ larvae have melanotic tumours. In addition, there is a highly reproducible and specific melanisation at the intersection between the midgut and posterior gut, in the imaginal ring region.

88% of mrn^EP3605/mrn⁵ larvae have melanotic tumours. In addition, there is a highly reproducible and specific melanisation at the intersection between the midgut and posterior gut, in the imaginal ring region.

mrn^EP3605/mrn¹ larvae show general necrosis.

Heterozygous larvae are more sensitive to UVB irradiation compared to controls.

Homozygous pupae are smaller than controls. mrn^EP3605/mrn³ adult escapers are smaller than controls.

Some homozygotes die at the pharate adult stage with deformations in the cuticle and a brittle bristle phenotype.

Early stages of oogenesis are normal but mature oocytes are deformed in homozygous females, apparently resulting from an aberrant chorion, and thus the eggs are not viable.

Aberrant egg chambers are seen in mrn^EP3605/mrn¹ and mrn^EP3605/mrn³ females and pycnotic nuclei are seen in nurse cells.

mrn^EP3605/mrn¹ males have defective testes, and spermatogenesis is either absent or not completed in most cases.

Mitotic spreads of mrn^EP3605/mrn¹ and mrn^EP3605/mrn⁵ larval neuroblasts show a high rate of chromosome aberrations, including fragmentation of chromosomes and partial chromosome condensation.

(Fregoso et al., 2007)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mrn^EP3605/mrn¹ has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn^EP3605/mrn³ has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn⁵/mrn^EP3605 has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn⁵/mrn^EP3605 has visible phenotype, suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn^EP3605/mrn³ has sterile phenotype, suppressible by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

mrn⁵/mrn^EP3605 has sterile phenotype, suppressible by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

NOT suppressed by

Statement

Reference

mrn^EP3605/mrn¹ has sterile phenotype, non-suppressible by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

mrn⁵/mrn^EP3605 has chaeta phenotype, suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn⁵/mrn^EP3605 has adult thorax phenotype, suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn⁵/mrn^EP3605 has adult abdomen phenotype, suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The defective bristle and thin thorax and abdomen phenotypes seen in mrn⁵/mrn^EP3605 adults are suppressed by Tfb5^Hsp83.PAF.

(Aguilar-Fuentes et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

(Fregoso et al., 2007)

(Fregoso et al., 2007)

(Fregoso et al., 2007)

Fails to complement

(Fregoso et al., 2007)

Rescued by

mrn^EP3605/mrn¹ is rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

mrn^EP3605 is rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

mrn^EP3605/mrn³ is rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

mrn⁵/mrn^EP3605 is rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tfb2^EP3605

mrn^EP3605

p52^EP3605

(Cruz-Becerra et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections