FlyBase Allele Report: Dmel\Nrg[l7]

General Information

Symbol

Dmel\Nrg^l7

Species

D. melanogaster

Name

FlyBase ID

FBal0013172

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

Also Known As

Nrg¹⁷, nrg², l(1)VA142

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hortsch et al., 1989)

hypomorphic allele - genetic evidence

(Hall and Bieber, 1997, Bieber et al., 1989)

Mutagen

ethyl methanesulfonate

(Hall and Bieber, 1997, Bieber et al., 1989, Hortsch et al., 1989)

Progenitor genotype

Cytology

Description

Amino acid replacement: W80term.

(Yamamoto et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,544,885..8,544,885 [+]

Nucleotide change:

G8544885A

Amino acid change:

Reported amino acid change:

W80term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid chang

(Yamamoto et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Chen et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | embryonic stage

(Lu et al., 2014)

dendritic tree, with Nrg^UAS.Tag:HA, Scer\GAL4^pk-IG1-1

(Yamamoto et al., 2006)

dorsal multidendritic neuron ddaD & axon

(Yamamoto, 2006, Yamamoto et al., 2006)

dorsal multidendritic neuron ddaD & dendritic tree

(Yamamoto, 2006, Yamamoto et al., 2006)

dorsal multidendritic neuron ddaE & axon

(Yamamoto, 2006, Yamamoto et al., 2006)

dorsal multidendritic neuron ddaE & dendritic tree

(Yamamoto et al., 2006)

embryonic/larval neuromuscular junction | embryonic stage

(Lu et al., 2014)

embryonic ganglionic branch & embryonic tracheole

(Paul et al., 2003)

larval abdominal segmental nerve

(Hall and Bieber, 1997)

larval DA1 motor neuron

(Hall and Bieber, 1997)

larval dorsal multidendritic neuron ddaE, with Nrg^UAS.Tag:HA, Scer\GAL4^pk-IG1-1

(Yamamoto et al., 2006)

larval intersegmental nerve

(Zarin et al., 2014)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Lu et al., 2014)

peripheral nervous system

(Hall and Bieber, 1997)

tracheal dorsal trunk primordium

(Paul et al., 2003)

Detailed Description

Statement

Reference

Nrg^l7/Nrg^l7 embryos display a synaptic defect similar to that seen in mir-8^Δ/mir-8^Δ (ISNb motor axons innervation defect at m6/7), though with reduced penetrance.

(Lu et al., 2014)

The olfactory receptor neurons of Nrg^l7 mutant flies do not show any overt phenotype, either in uninjured flies or in flies in which the antennae have been surgically ablated.

(Wishart et al., 2012)

56% of dorsal da neuron E (ddaE) and 14% of ddaD neurons show ectopic branches on their axons at 19-20 hours after egg laying (AEL) in Nrg^l7 embryos. In addition, the dendritic arbors of ddaD and ddaE are deformed and this phenotype can be detected at 18-19 hours AEL.

Embryos that express Nrg^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^IG1-1, in a Nrg^l7 background and Scer\GAL4^repo show deformed ddaE dendritic arbors.

(Yamamoto et al., 2006)

56% of dorsal da neuron E (ddaE) and 14% of ddaD neurons show ectopic branches on their axons in Nrg^l3 embryos. The dendritic branches of ddaD cover a smaller field and have fewer terminals than wild-type ddaD neurons.

(Yamamoto, 2006)

Nrg^l10/Nrg^l7 females are viable at 18[o]C but are inviable at 25[o]C.

Nrg⁸⁴⁹/Nrg^l7 and Nrg⁸⁹²/Nrg^l7 females show normal sexual receptivity when mated to wild-type males.

(Carhan et al., 2005)

In Nrg^l7 homozygous embryos, tracheal phenotypes are apparent from stage 15. At stage 16 in these embryos the average dorsal trunk length is significantly greater than wild type (P<0.005) (121+/-2% mean+/-s.e.m., n>5, normalized to stage 16 wild-type value). In addition these embryos have moderately severe diameter increases in the dorsal trunk and other primary tracheal branches, and some ganglionic branches exhibit missing lumen. Unlike wild-type, post stage 15 trachea in Nrg^l7 homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier.

(Paul et al., 2003)

Homozygous embryos show a general disorganisation of the peripheral nervous system cell bodies. The aCC and SNb motoneurons often show a stalled phenotype, failing to extend normally, and SNb motoneurons sometimes show abnormal contacts with their targets.

(Hall and Bieber, 1997)

Nrg^l7 is lethal during the late embryonic and early larval phase of development. It has no maternal effect component.

(Bieber et al., 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nrg^l7 has abnormal neuroanatomy phenotype, enhanceable by eve^ΔRP2A

(Zarin et al., 2014)

Enhancer of

Statement

Reference

Nrg^l7/Nrg[+] is an enhancer of abnormal neuroanatomy | larval stage phenotype of Nak^RNAi.UAS, Scer\GAL4^elav-C155

(Yang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nrg^l7 has larval intersegmental nerve phenotype, enhanceable by eve^ΔRP2A

(Zarin et al., 2014)

Enhancer of

Statement

Reference

Nrg^l7/Nrg[+] is an enhancer of dendritic arborizing neuron | larval stage phenotype of Nak^RNAi.UAS, Scer\GAL4^elav-C155

(Yang et al., 2011)

Nrg^l7/Nrg[+] is an enhancer of dendrite | larval stage phenotype of Nak^RNAi.UAS, Scer\GAL4^elav-C155

(Yang et al., 2011)

Nrg^l7 is an enhancer of phenotype of Nrt⁵

(Speicher et al., 1998)

Nrg^l7 is an enhancer of axon | embryonic stage phenotype of Nrt¹

(Speicher et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Removing one copy of eve in Nrg^l7/Y mutants results in more severe intersegmental nerve guidance defects with a significant increase in axon stalling upon the addition of eve^ΔRP2A.

(Zarin et al., 2014)

Nrg^l7 dominantly enhances the shortening of dendritic length and the reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Yang et al., 2011)

Increases the frequency of Fas2 expressing axons showing defects in Nrt¹/Nrt⁵, Nrt¹/Nrt¹ and Nrt⁵/Nrt⁵ embryos.

(Speicher et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Nrg^ibx

(Carhan et al., 2005)

Fails to complement

Nrg^l10

(Hall and Bieber, 1997)

Nrg^l4

(Hall and Bieber, 1997)

Rescued by

Nrg^l7 is rescued by Nrg^+t25

(Enneking et al., 2013)

Nrg^l7 is rescued by Nrg^167ΔFIGQY

(Enneking et al., 2013)

Nrg^l7 is rescued by Nrg^180ΔFIGQY

(Enneking et al., 2013)

Nrg^l7 is rescued by Nrg^{Ubi-p63E.Tag:HA}

(Yamamoto et al., 2006)

Partially rescued by

Nrg^l7 is partially rescued by Scer\GAL4^repo/Scer\GAL4^pk-IG1-1/Nrg^UAS.Tag:HA

(Yamamoto et al., 2006)

Not rescued by

Nrg^l7 is not rescued by Nrg^ΔIg3-4

(Enneking et al., 2013)

Comments

Expression of Nrg^+t25 fully rescues Nrg^l7 mutant embryonic lethality.

Expression of Nrg^167ΔFIGQY fully rescues Nrg^l7 mutant embryonic lethality.

Expression of Nrg^180ΔFIGQY fully rescues Nrg^l7 mutant embryonic lethality.

Expression of Nrg^ΔIg3-4 fails to rescue Nrg^l7 mutant embryonic lethality.

(Enneking et al., 2013)

The reduced female receptivity phenotype of Nrg^ibx is complemented by Nrg^l7.

Nrg^l7 complements the reduced female receptivity phenotype of Nrg⁸⁹².

(Carhan et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (2)

5595

108320

Notes on Origin

Discoverer

Lefevre.

Comments

Complements: ibx^M72.

(Kerr et al., 1997)

Maternal germline clonal analysis demonstrates there is no maternal effect.

(Perrimon et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Nrg¹⁷

(Hall et al., 2014, Enneking et al., 2013, Read et al., 2005, Paul et al., 2003)

Nrg²

(Goossens et al., 2011)

Nrg⁷

(Kerr et al., 1997)

Nrg^I7

(Tempesta et al., 2017)

Nrg^l7

(Byri et al., 2015, Bätz et al., 2014, Mao and Freeman, 2009)

VA142

(Hortsch et al., 1989, Perrimon et al., 1989)

l(1)7Fa⁷

l(1)VA142

(Kerr et al., 1997, Bieber et al., 1989)

nrg¹⁷

(Chen et al., 2023, Lu et al., 2014, Yang et al., 2011, Stork et al., 2009)

nrg²

(Zarin et al., 2014, Yamamoto, 2006, Yamamoto et al., 2006, Carhan et al., 2005, Speicher et al., 1998, Hall and Bieber, 1997)

Name Synonyms

Secondary FlyBase IDs

References (24)

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