FlyBase Allele Report: Dmel\ogre[2]

General Information

Symbol

Dmel\ogre²

Species

D. melanogaster

Name

FlyBase ID

FBal0013232

Feature type

allele

Associated gene

Dmel\ogre

Associated Insertion(s)

Carried in Construct

Also Known As

ogre^cb8

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lipshitz and Kankel, 1985)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Curtin et al., 2002)

abnormal neurophysiology | adult stage

(Curtin et al., 2002)

lethal | recessive | heat sensitive

(Lipshitz and Kankel, 1985)

viable

(Lipshitz and Kankel, 1985)

Phenotype Manifest In

adult optic lobe

(Lipshitz and Kankel, 1985)

central nervous system

(Lipshitz and Kankel, 1985)

lamina monopolar neuron

(Curtin et al., 2002)

photoreceptor neuron

(Curtin et al., 2002)

Detailed Description

Statement

Reference

In ogre² mutants, the receptor potential is normal, indicating normal photoreceptor cell transduction and depolarisation. However ogre² mutants show an absence of transients, indicating that the lamina is not receiving its normal input from the retina.

(Curtin et al., 2002)

ogre² flies show no electroretinogram (ERG) transients, although the magnitude of the receptor potential is completely normal. ogre² transients are absent in both light and dark-adapted conditions. Mosaic ogre² flies, in which the eye is completely mutant for ogre, but all other body tissues, including the lamina are heterozygous (i.e. phenotypically wild-type), completely lack on- and off-transients when dark adapted. These mosaic animals differ from ogre² homozygous mutants in having normal-size optic ganglia. In ogre² mutants, the R1-R6 neurons correctly innervate the lamina and do not exhibit obvious pathfinding defects, although in 10% of cases occasional small gaps are observed in the staining pattern of R1-R6 (visualised with Ecol\lacZ^{ninaE.T:Btau\MAPT}), this is not observed in control stainings.

(Curtin et al., 2002)

Viable. Abnormal pattern preference behaviour, normal eye morphology and highly abnormal optic lobes. Gynandromorph analysis and somatic recombinant mosaics demonstrate that the eye alone does not induce the optic lobe phenotype. ogre¹/ogre² are temperature sensitive lethals: 60% survive at 29^oC and 12% at 18^oC. Adult escapers of ogre² heterozygotes with lethal alleles of ogre have an extreme optic lobe phenotype and holes in the CNS.

(Lipshitz and Kankel, 1985)

viable

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ogre² has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^elav-C155/ogre::shakB^{o1-182:s173-end.UAS}

(Curtin et al., 2002)

ogre² has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^elav-C155/ogre::shakB^{o1-182:s173-241:o252-end.UAS}

(Curtin et al., 2002)

NOT suppressed by

Statement

Reference

ogre² has abnormal neurophysiology phenotype, non-suppressible by shakB^N.UAS/Scer\GAL4^ogre.PC

(Curtin et al., 2002)

ogre² has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ogre.PC/Rnor\Gja1^UAS.cCa

(Curtin et al., 2002)

ogre² has abnormal neurophysiology phenotype, non-suppressible by shakB^L.UAS/Scer\GAL4^ogre.PC/Scer\GAL4^hs.2sev

(Curtin et al., 2002)

NOT Suppressor of

Statement

Reference

ogre² is a non-suppressor of abnormal neurophysiology phenotype of Scer\GAL4^ogre.PC, shakB^L.UAS

(Curtin et al., 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

ogre::shakB^{o1-182:s173-241:o252-end.Scer\UAS} rescues ogre² effectively, rescuing the on-transient 63% and the off-transient 64% as effectively as ogre² protein. ogre::shakB^{o1-182:s173-end.Scer\UAS} rescues ogre² effectively, rescuing the on-transient 50% and the off-transient 66% as effectively as ogre² protein.

(Curtin et al., 2002)

shakB^L.Scer\UAS expression in the retinal neurons of ogre² mutants, under the simultaneous regulation of both Scer\GAL4^ogre.PC and Scer\GAL4^hs.2sev does not rescue the electroretinogram phenotype of ogre² mutants.

shakB^L.Scer\UAS expression in the retinal neurons of ogre² mutants, under the simultaneous regulation of both Scer\GAL4^ogre.PC and Scer\GAL4^unspecified does not rescue the electroretinogram phenotype of ogre² mutants.

(Curtin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ogre² is rescued by ogre^UAS.cCa/Scer\GAL4^ogre.PC

(Curtin et al., 2002)

ogre² is rescued by ogre^UAS.cCa/Scer\GAL4^ogre.PC/Scer\GAL4^hs.2sev

(Curtin et al., 2002)

Partially rescued by

ogre² is partially rescued by Scer\GAL4^elav-C155/ogre^UAS.cCa

(Curtin et al., 2002)

ogre² is partially rescued by ogre^UAS.cCa/Scer\GAL4^hs.2sev

(Curtin et al., 2002)

ogre² is partially rescued by ogre^UAS.cCa/Scer\GAL4^ogre.PC

(Curtin et al., 2002)

Not rescued by

ogre² is not rescued by ogre^UAS.ΔC.cCb/Scer\GAL4^ogre.PC

(Curtin et al., 2002)

Comments

The ogre² mutant phenotype can be rescued by expression of wild-type ogre^Scer\UAS.cCa under a Scer\GAL4^ogre.PC driver. This driver expresses in a subset of retinal axons beginning in the early pupal stage. Double copies of both Scer\GAL4^ogre.PC and ogre^Scer\UAS.cCa are needed to effect adequate rescue. Animals containing two copies of both ogre^Scer\UAS.cCa and Scer\GAL4^ogre.PC show rescue of on-transients that are approximately 80% of wild-type and off-transients that are approximately 70% of wild-type levels.

(Curtin et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lipshitz.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CB8

(Kankel and Lipshitz, 1981)

ogre²

(Matsunaga et al., 2017)

ogre^cb8

(Curtin et al., 2002, Curtin et al., 2002)

ogre^vcb8

(Lipshitz and Kankel, 1985)

Name Synonyms

viable contrast blind

Secondary FlyBase IDs

References (6)

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