FlyBase Allele Report: Dmel\otu[1]

General Information

Symbol

Dmel\otu¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013348

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Also Known As

fs231

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Oliver et al., 1993)

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

female germline cell | decreased number

(Mercer et al., 2025)

ovariole

(Mercer et al., 2025, Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Bae et al., 1994, Wei et al., 1994, Bopp et al., 1993)

ovary | adult stage

(Partanen et al., 1991)

stretch follicle cell

(Tirronen et al., 1993)

Detailed Description

Statement

Reference

Homozygous females have a shorter mean life span compared to heterozygous controls. The lifespan of mated homozygous females is significantly shorter than that of unmated homozygous females.

(Ueyama and Fuyama, 2003)

Tumorous phenotype.

(Rodesch et al., 1995)

Tumorous ovaries.

(Bae et al., 1994)

Many more germ cells in ovarian tumors are positive for expression of male germline markers than in wild type testes, suggesting a relaxation of growth control, or accumulation of apical cells due to a developmental block.

(Wei et al., 1994)

Tumour generating, some egg chambers develop with variable numbers of pseudo-nurse cells. Double mutants with qui¹ and egl⁵ display an otu¹ phenotype, otu¹ is epistatic over qui¹ and egl⁵.

(Tirronen et al., 1993)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

Tumorous ovaries. Oogenesis is normal in homozygotes when in combination with bgcn^QS2 heterozygotes.

(Partanen et al., 1991)

92.5% of homozygous ovarioles have tumorous oogonia. otu¹¹/otu¹ female flies are poorly fertile.

(Storto and King, 1987)

Oncogenic allele; 91% of homozygous ovarioles contain tumorous chambers consisting of a mass of small, undifferentiated, mitotically active cells. 85% of hemizygous ovarioles are quiescent.

(King et al., 1986)

otu¹ behaves like a DIF allele at 18^oC, an ONC allele at 23^oC and a QUI allele at 28^oC. The ovarian tumors which give the mutant gene its name are made up of large numbers of single cystocytes and small numbers of clones of 2-4 interconnected cells.

(King, 1979)

oncogenic; cystocytes continue dividing and produce tumors

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

otu¹ has decreased cell number | oogenesis phenotype, non-suppressible by Scer\GAL4^nanos.PU/bbn^UASp.cMa

(Mercer et al., 2025)

Suppressor of

Statement

Reference

otu[+]/otu¹ is a suppressor of abnormal mitotic cell cycle phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

otu¹ has female germline cell | decreased number phenotype, non-suppressible by Scer\GAL4^nanos.PU/bbn^UASp.cMa

(Mercer et al., 2025)

otu¹ has ovariole phenotype, non-suppressible by Scer\GAL4^nanos.PU/bbn^UASp.cMa

(Mercer et al., 2025)

Suppressor of

Statement

Reference

otu[+]/otu¹ is a suppressor of egg chamber phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The extra germline cell phenotype seen in enc^UU3 egg chambers is suppressed by otu¹/+.

(Van Buskirk and Schupbach, 2002)

otu¹ does not have an obvious effect on the abundance of germ cells in XY tra^F.Hsp83 pseudo-ovaries.

(Waterbury et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

otu¹ is rescued by otu^{S40A.UASp.EGFP}/Scer\GAL4^nanos.PU

(Mercer et al., 2025)

otu¹ is rescued by otu^UASp.EGFP/Scer\GAL4^nanos.PU

(Mercer et al., 2025)

otu¹ is rescued by otu^+t5.4

(Comer et al., 1992)

Partially rescued by

otu¹ is partially rescued by otu⁴²³

(Glenn and Searles, 2001)

otu¹ is partially rescued by otu⁶²⁷

(Glenn and Searles, 2001)

otu¹ is partially rescued by otu^hs.PN

(Rodesch et al., 1995)

Not rescued by

otu¹ is not rescued by otu³³⁸

(Glenn and Searles, 2001)

otu¹ is not rescued by otu²⁵⁰

(Glenn and Searles, 2001)

otu¹ is not rescued by otu³⁸⁰

(Glenn and Searles, 2001)

Comments

One or two copies of P{hs-otu.N} causes substantial morphological suppression but only partial suppression of sterility.

(Rodesch et al., 1995)

Rescued by otu^+t5.4 : fertility and normal ovarian development is restored.

(Comer et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3022

ctⁿ otu¹ v²⁴/FM3

Kyoto

106786

ctⁿ otu¹ v²⁴/FM3

Notes on Origin

Discoverer

Gans.

Comments

Oncogenic (ONC) class allele. The ovarian phenotype of flies transheterozygous for otu¹ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

One of ONC class of alleles.

(Comer et al., 1992)

Expression of Sxl is severely reduced in germ cells of homozygous females. Substantial levels of male-type splicing can be detected in the mutant ovaries.

(Bopp et al., 1993)

Severe otu allele.

(Tirronen et al., 1993)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles. Reverse transcription and PCR, and in situ hybridization with the male specific exon, showed that the Sxl transcripts are spliced in the male-specific mode in transformed germ cells. This effect is alleviated by the Sxl^M1 mutation.

(Pauli et al., 1993)

Penetrance of interaction with otu¹ is 73%.

(Partanen et al., 1991)

The banding patterns of the polytene chromosomes of ovarian pseudonurse cells of otu¹ females have been compared with larval salivary gland polytene chromosomes.

(Heino, 1994)

ONC class of otu allele. P{otu-98} restores a low but detectable level of fertility, P{otu-104} completely restores fertility.

(Sass et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

fs(1)231

(Perrimon et al., 1986)

fs(1)A231

fs231

(King and Riley, 1982, King et al., 1978)

otu¹

(Mercer et al., 2025, Ji et al., 2019, Ji et al., 2017, Yang et al., 2012, Popodi et al., 2010-, Sun and Cline, 2009, Ueyama and Fuyama, 2003)

Name Synonyms

Secondary FlyBase IDs

References (36)

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