FlyBase Allele Report: Dmel\pum[680]

General Information

Symbol

Dmel\pum⁶⁸⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0014084

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

Also Known As

pum¹³, pumilio⁶⁸⁰

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schweers et al., 2002, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: G1330D.

(Schweers et al., 2002)

Amino acid replacement: G1330D. G1330D falls within the RNA-binding domain.

(Wharton et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,071,627..9,071,627 [-]

Nucleotide change:

G9071627A

Amino acid change:

Reported amino acid change:

G1330D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wharton et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | recessive

(Ghezzi et al., 2013)

female sterile | recessive

(Schweers et al., 2002)

lethal | maternal effect | recessive | heat sensitive

(Lehmann and Nusslein-Volhard, 1991)

visible | recessive

(Kim et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous pum¹³ significantly blocks or reduces tolerance to both benzyl alcohol and ethanol.

(Ghezzi et al., 2013)

The notum of homozygous pum¹³ flies display extra macrochaetae. The penetrance of the notum phenotype is incomplete.

(Kim et al., 2012)

pum¹³ mutants show abdominal defects in the larva. Medial protrusions and exposed sclerites are also seen in the larva head. Head skeleton defects and head involution defects are seen. 95% of homozygous larvae fail develop the normal mouth hook projection. 98% of pum¹³/pum¹ larvae fail to develop the normal mouth hook projection.

(Gamberi et al., 2002)

Most embryos laid by pum^Msc/pum¹³ mothers have 2 or 3 abdominal segments rather than the normal 8.

(Sonoda and Wharton, 2001)

Germ cells are present in embryos derived from homozygous females.

(Heller and Steinmann-Zwicky, 1998)

Embryos derived from homozygous embryos have 0 to 2 abdominal segments. This phenotype is largely rescued if the females carry pum^nos.PB, and is slightly rescued if the females carry pum^Δ2'.nos or pum^Δ5'.nos. Most of the embryos derived from pum¹³/+ females carrying pum^G1330D.nos have abdominal segmentation defects.

(Wharton et al., 1998)

Has no effect on the posterior accumulation of G-iα65A protein.

(Wolfgang and Forte, 1995)

pum¹³ mutant embryos typically form only two abdominal segments. pum¹³;6xP{osk⁺108} embryos exhibit a wild type cuticular pattern and some hatch to produce viable adults.

(Smith et al., 1992)

Temperature sensitivity is restricted to the abdominal phenotype.

(Lehmann and Nusslein-Volhard, 1991)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Large amounts of vas protein are expressed in early stages of oogenesis. vas protein localizes to the posterior pole at the same stage of egg development as wild type.

(Lasko and Ashburner, 1990)

Homozygous embryos derived from homozygous females have polar granules and pole cells and have deletions of abdominal structures. CycB and vas transcript localization and accumulation is as in wild type.

(Raff et al., 1990)

Homozygous females produce embryos that have a maximum of two abdominal segments. The head, thorax and telson are normal, and pole cells are formed. Embryos derived from females mutant for both tsl^unspecified and pum¹³ develop several abdominal segments.

(Lehmann and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pum⁶⁸⁰ is an enhancer of lethal phenotype of Scer\GAL4^HD34A, nanos^{αTub84B.3'UTR.UAS}

(Duchow et al., 2005)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

pum⁶⁸⁰ has eye phenotype, non-enhanceable by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

pum⁶⁸⁰ has retina phenotype, non-enhanceable by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT suppressed by

Statement

Reference

pum⁶⁸⁰ has eye phenotype, non-suppressible by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

pum⁶⁸⁰ has retina phenotype, non-suppressible by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT Enhancer of

Statement

Reference

pum⁶⁸⁰ is a non-enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

pum⁶⁸⁰ is a non-enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Suppressor of

Statement

Reference

pum[+]/pum⁶⁸⁰ is a suppressor | partially of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum⁶⁸⁰/pum⁶⁸⁰ is a suppressor | partially of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum⁶⁸⁰/pum⁶⁸⁰ is a suppressor | partially of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum⁶⁸⁰/pum⁶⁸⁰ is a suppressor | partially of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum⁶⁸⁰/pum⁶⁸⁰ is a suppressor | partially of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum⁶⁸⁰/pum⁶⁸⁰ is a suppressor | partially of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum[+]/pum⁶⁸⁰ is a suppressor | partially of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum[+]/pum⁶⁸⁰ is a suppressor | partially of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum[+]/pum⁶⁸⁰ is a suppressor | partially of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

pum[+]/pum⁶⁸⁰ is a suppressor | partially of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

NOT Suppressor of

Statement

Reference

pum⁶⁸⁰ is a non-suppressor of wing phenotype of Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.UAS}

(Clark et al., 2002)

pum⁶⁸⁰ is a non-suppressor of adult cuticle phenotype of Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.UAS}

(Clark et al., 2002)

pum⁶⁸⁰ is a non-suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

pum⁶⁸⁰ is a non-suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The ovariole/egg chamber defects observed in l(3)mbt^GM76/Df(3R)ED10966 females are weakly and strongly suppressed by pum⁶⁸⁰ heterozygosity and homozygosity, respectively.

(Coux et al., 2018)

The frequency of the adolescent phenotype in animals expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^sca-109-68 is not altered if they are also carrying pum¹³.

(Clark et al., 2002)

Injection of nos^N5 RNA into homozygous embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pum^bem

(Schweers et al., 2002)

Partially rescued by

pum⁶⁸⁰ is partially rescued by pum^Δ5'.nanos

(Wharton et al., 1998)

pum⁶⁸⁰ is partially rescued by pum^nanos.PB

(Wharton et al., 1998)

pum⁶⁸⁰ is partially rescued by pum^Δ2'.nanos

(Wharton et al., 1998)

Comments

Complementation tested against pum^bem female sterility phenotype.

(Schweers et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

3332

kni⁹ pum⁶⁸⁰/TM3, Ser¹

3260

st¹ pum⁶⁸⁰/TM3, Sb¹ Ser¹

Kyoto

106984

kni⁹ pum⁶⁸⁰/TM3, Ser¹

106942

st¹ pum⁶⁸⁰ nanos^L7 e¹

106946

st¹ pum⁶⁸⁰/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Recovered on the basis of failure to complement the maternal phenotype.

(Lindsley and Zimm, 1992)

Comments

The abdominal phenotype of mutant embryos can be partially rescued by the injection of wild-type cytoplasm into the abdominal region. The rescuing activity acts directly at the site of injection and does not seem to spread; injections into the dorsal side of the prospective abdomen lead to the development of dorsal structures but leave the ventral side strongly mutant. Injections anterior and posterior to the abdominal region rarely lead to rescue. Rescuing activity is recovered only from the posterior pole of donor embryos. Cytoplasm transplanted from osk⁶ mutant embryos does not rescue the abdominal phenotype of pum¹³ mutant embryos.

(Lehmann and Nusslein-Volhard, 1987)

hb protein expression in early pum¹³ mutant embryos has been studied.

(Tautz, 1988)

The posterior localization of tud product is unaffected in mutant embryos.

(Bardsley et al., 1993)

Renamed from 'pum[13]' to 'pum[680]' to reflect the nomenclature that is: (i) used in the original publication (FBrf0047058); and (ii) much more commonly used in the published literature.

(FlyBase, 1992-)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pum¹³

(Miles et al., 2014, Ghezzi et al., 2013, Kim et al., 2012, Christensen et al., 2008.12.28)

pumilio⁶⁸⁰

(Jankovics et al., 2001, Tautz, 1988)

Name Synonyms

Secondary FlyBase IDs

References (46)

Report Sections

Open Close