FlyBase Allele Report: Dmel\rsh[1]

General Information

Symbol

Dmel\rsh¹

Species

D. melanogaster

Name

FlyBase ID

FBal0014391

Feature type

allele

Associated gene

Dmel\rsh

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Folkers et al., 2006)

Amino acid replacement: Q?term.

(Folkers et al., 2006)

Mutation is predicted to result in a truncated rad protein that is lacking the C-terminal 64 amino acid residues.

(Folkers et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:13,034,095..13,034,095 [+]

Nucleotide change:

C13034095T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q?term

Comment:

Site of nucleotide and amino acid substitution in mutant based on sequence presented in paper.

(Folkers et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Larnerd et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart | P-stage

(Johnson et al., 2002)

axon | third instar larval stage

(Guan et al., 2011)

varicosity | third instar larval stage

(Guan et al., 2011)

Detailed Description

Statement

Reference

rad¹ homozygous adults have significantly reduced 24-hour associative olfactory water-reward memory, compared to controls, with no effect on 3-min or 3-hour memory.

(Shyu et al., 2017)

rad¹ mutant adults have significantly reduced 3-hour aversive olfactory anaesthesia-resistant memory, compared to controls, with no effect on odor or shock avoidance.

(Yang et al., 2016)

Compared with wild-type, rad¹ mutants display an increase in varicosity number and a decrease in innervation length, resulting in a compact innervation pattern at the third instar larval neuromuscular junction.

(Guan et al., 2011)

Three-hour memory impairment after cold-induced anesthesia is reduced further in rad¹ mutants. Reducing 5HT levels by pCPA feeding has no effect in rad¹ mutants.

(Lee et al., 2011)

Extinction learning in rad¹ mutants, with either 10 or 30 cycles of training is no different from wild-type controls.

(Qin and Dubnau, 2010)

rad¹ flies exhibit a reduced sugar response compared to wild-type Canton-S flies.

(Colomb et al., 2009)

Mutant pupae show bradycardia. At every temperature tested, the mutant heart rate is significantly lower than that of wild type. This strain is insensitive to both serotonin and norepinephrine.

(Johnson et al., 2002)

rad¹ flies can learn better than wild-type controls in a standard training procedure. Memory retention is not significantly affected by the rad¹ mutation.

(Gong et al., 1998)

In wild-type animals, increasing the density at which larvae are reared results in an increase in the volume of the calyx in the adult brain. This plasticity is still seen in rad¹ mutants.

(Hitier et al., 1998)

Anesthesia resistant memory is disrupted. One day retention after massed training is zero, and after spaced training is subnormal. rad¹ mutants fed cycloheximide showed zero 1 day retention after spaced training. However, 2-7 day retention after spaced training is similar to that of wild type flies.

(Tully et al., 1994)

Individuals learn normally but display abnormally rapid memory decay after training in the olfactory discrimination test of Tully and Quinn (Tully, J. Comp. Physiol. A 157: 263). Memory loss decays to zero.

(Folkers et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rsh¹ has abnormal memory | adult stage phenotype, enhanceable by Scer\GAL4^VT030604/Octβ2R^KK109808

(Yang et al., 2016)

rsh¹ has abnormal memory | adult stage phenotype, enhanceable | heat sensitive by shi^1.UAS/Scer\GAL4^VT030604

(Yang et al., 2016)

NOT Enhanced by

Statement

Reference

rsh¹ has abnormal memory | adult stage phenotype, non-enhanceable by Octβ2R^KK109808/Scer\GAL4^VT049246

(Yang et al., 2016)

rsh¹ has abnormal memory | adult stage phenotype, non-enhanceable by shi^1.UAS/Scer\GAL4^VT049246

(Yang et al., 2016)

NOT suppressed by

Statement

Reference

rsh¹ has abnormal memory | adult stage phenotype, non-suppressible by Octβ2R^KK109808/Scer\GAL4^VT049246

(Yang et al., 2016)

rsh¹ has abnormal memory | adult stage phenotype, non-suppressible by shi^1.UAS/Scer\GAL4^VT049246

(Yang et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rsh¹ is rescued by rsh^hs.PF

(Folkers et al., 2006)

Comments

Expression of rad^hs.PF, following heat shock, rescues the disrupted anesthesia resistant memory phenotype of rad¹ flies.

(Folkers et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79209

w¹¹¹⁸ rsh¹

Notes on Origin

Discoverer

Comments

rad flies have good initial learning followed by rapid memory decay. memory decay is faster than that for amn¹ flies. Cold-induced anaesthesia treatment abolishes memory performance. Behavioural assays demonstrate that flies cannot smell odours or sense electric shocks.

(Folkers et al., 1993)

Memory assayed in a classical Pavlovian conditioning procedure for odor-avoidance.

(Tully et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

rad

(Gong et al., 1998)

rad¹

(Larnerd et al., 2023, Chiang et al., 2007)

radish¹

(Song et al., 2019)

rsh

(Hitier et al., 1998)

rsh¹

(Song et al., 2019, Shyu et al., 2017, Yang et al., 2016, Chen et al., 2015, Wu et al., 2013, Guan et al., 2011, Lee et al., 2011, van Swinderen, 2007)

Name Synonyms

radish1

(Qin and Dubnau, 2010)

Secondary FlyBase IDs

References (21)

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