FlyBase Allele Report: Scer\GAL4[VT049246]

General Information

Symbol

Scer\GAL4^VT049246

Species

S. cerevisiae

Name

FlyBase ID

FBal0307516

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VT049246-GAL4}

Also Known As

VT49246-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Meadows, 2015.3.13)

Progenitor genotype

Carried in construct

P{VT049246-GAL4}

Cytology

Description

A 2154 base pair fragment from the flanking non-coding or intronic region of jus is fused upstream of a Drosophila synthetic core promoter (DSCP) followed by sequence encoding a GAL4 driver.

(Meadows, 2015.3.13)

Allele components

Component

Use(s)

Regulatory region(s)

VT049246

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Marker for

alpha/beta Kenyon cell

(Wu et al., 2023, Wu et al., 2018)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Inx5^GD1177

(Shyu et al., 2019)

abnormal memory | adult stage, with Inx5^JF02877

(Shyu et al., 2019)

abnormal memory | adult stage, with rsh^GD8769

(Yang et al., 2016)

abnormal memory | adult stage | conditional, with Npha\HR^{eNpHR(Unk).UAS.YFP.cUa}

(Shyu et al., 2019)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Yang et al., 2016)

abnormal memory | adult stage | heat sensitive, with shi^{ts1.IVS.p10.20xUAS}

(Shyu et al., 2017)

long lived, with Rab27^JF02165

(Lien et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

Hyperpolarization (using Npha\HR^{eNpHR(Unk).UAS.YFP.cUa} with 590 nm (yellow) light) of Scer\GAL4^VT049246 cells in adults during testing significantly reduces the residual anaesthesia-resistant 3 hour aversive olfactory memory after cold-shock, compared to controls.

(Shyu et al., 2019)

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 32[o]C) of Scer\GAL4^VT049246 cells in adults during retrieval alone, but not acquisition and consolidation, significantly reduces 24-hour associative olfactory water-reward memory, compared to controls; inhibition has no effect on odor acuity or water preference, compared to controls.

(Shyu et al., 2017)

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^VT049246 cells in adults during testing only significantly reduces 3-hour aversive olfactory anaesthesia-resistant memory, compared to controls, with no effect on odor or shock avoidance.

(Yang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Octβ2R^KK109808/Scer\GAL4^VT049246 is a non-enhancer of abnormal memory | adult stage phenotype of rsh¹

(Yang et al., 2016)

shi^1.UAS/Scer\GAL4^VT049246 is a non-enhancer of abnormal memory | adult stage phenotype of rsh¹

(Yang et al., 2016)

NOT Suppressor of

Statement

Reference

Octβ2R^KK109808/Scer\GAL4^VT049246 is a non-suppressor of abnormal memory | adult stage phenotype of rsh¹

(Yang et al., 2016)

shi^1.UAS/Scer\GAL4^VT049246 is a non-suppressor of abnormal memory | adult stage phenotype of rsh¹

(Yang et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^VT049246 cells during testing only does not alter the reduction in 3-hour aversive olfactory anaesthesia-resistant memory (ARM) seen in rad¹ mutant adults.

(Yang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^VT049246

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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