FlyBase Allele Report: Dmel\rsh[GD8769]

General Information

Symbol

Dmel\rsh^GD8769

Species

D. melanogaster

Name

FlyBase ID

FBal0201478

Feature type

allele

Associated gene

Dmel\rsh

Associated Insertion(s)

Carried in Construct

P{GD8769}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8769}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8769

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^VT049246

(Yang et al., 2016)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of rad^GD8769 under the control of Scer\GAL4^VT049246, but not Scer\GAL4^VT030604 or Scer\GAL4^VT044966, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces 3-hour aversive olfactory anaesthesia-resistant memory, compared to controls.

(Yang et al., 2016)

Adults expressing rad^GD8769 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of rad^GD8769 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD8769} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or early pupal lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 100% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8769

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

rsh^GD8769/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2012)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

rsh^GD8769/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of rad^GD8769 does not suppress the rough eye phenotype seen when Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^GMR.PU.

(Qurashi et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v39932

w¹¹¹⁸; P{GD8769}v39932

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8769

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG15720^GD8769

Rad^v39931

(Qurashi et al., 2012)

Rad^v39932

(Qurashi et al., 2012)

rad^GD8769

rsh^GD8769

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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