FlyBase Allele Report: Dmel\Met[1]

General Information

Symbol

Dmel\Met¹

Species

D. melanogaster

Name

FlyBase ID

FBal0014802

Feature type

allele

Associated gene

Dmel\Met

Associated Insertion(s)

Carried in Construct

Also Known As

Met

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wilson, 1996)

Mutagen

ethyl methanesulfonate

(Ashok et al., 1998, Restifo and Wilson, 1998, Wilson, 1996, Minkoff and Wilson, 1992, Shemshedini and Wilson, 1990, Wilson and Fabian, 1986)

Progenitor genotype

Cytology

Description

Amino acid replacement: G166D.

(Wilson et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,617,364..11,617,364 [+]

Nucleotide change:

G11617364A

Amino acid change:

G166D | Met-PA; G166D | Met-PB

Reported amino acid change:

G166D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wilson et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Wilson et al., 2006, Ashok et al., 1998)

chemical resistant | dominant

(Wilson, 1996)

chemical resistant | semidominant

(Wilson and Fabian, 1986)

visible

(Wilson et al., 2006)

Phenotype Manifest In

eye

(Wilson et al., 2006)

Detailed Description

Statement

Reference

Homozygotes show increased resistance to methoprene compared to controls.

18% of mutant flies have a small number of defective ommatidia in the posterior quarter of the eye.

(Wilson et al., 2006)

Homozygotes fed 100μl methoprene have almost normal optic lobes, except for a very small defect in the right outer chiasm, in contrast to wild-type flies, which show widespread degeneration of the optic lobes after feeding with 100μl methoprene. In addition, homozygotes occasionally show very mild subesophageal ganglion-thoracic ganglion (SEG-TG) fusion defects when fed 100μl methoprene, in contrast to wild-type flies, which show severe SEG-TG fusion defects when fed 100μl methoprene. Homozygotes have slightly reduced numbers of muscle fibres in the retractor muscles of the rostrum of the proboscis when fed 100μl methoprene.

(Restifo and Wilson, 1998)

Homozygous, heterozygous, hemizygous and Rst(1)JH^D29/Rst(1)JH¹ larvae show increased resistance to Methoprene compared to wild-type. Homozygous, hemizygous and Rst(1)JH^D29/Rst(1)JH¹ adults derived from larvae treated with Methoprene have normal bristle patterns on sternites 6 and 7, in contrast to wild-type adults derived from larvae treated with Methoprene, which have disrupted bristle patterns on these sternites.

(Wilson, 1996)

Flies carrying Rst(1)JH¹ have a competitive disadvantage compared to wild-type flies. Pupal survival is reduced compared to wild-type.

(Minkoff and Wilson, 1992)

Rst(1)JH¹ mutants are 10 times more resistant than wild-type to S31183 (2-^{1-methyl-2-(4-phenoxyphenoxy)-ethoxy}-pyridine), a juvenile hormone mimic, in a white puparial assay and about 20 times more resistant than wild-type to S31183 in a larval feeding assay.

(Riddiford and Ashburner, 1991)

Strong allele. Only minor differences in penetration, excretion, sequestration and metabolism of juvenile hormone III are detected in Rst(1)JH¹ flies compared to Rst(1)JH⁺ flies. Rst(1)JH¹ flies have an approximately 10-fold lower binding affinity for juvenile hormone III than Rst(1)JH⁺ flies.

(Shemshedini and Wilson, 1990)

Juvenile hormone III binding affinity and total protein accumulation in the male accessory glands is reduced.

(Shemshedini et al., 1990)

Slightly stronger than Rst(1)JH². Homozygotes are nearly two orders of magnitude (100x) more resistant to methoprene and juvenile hormone III than wild-type. The resistance of heterozygotes is intermediate between the homozygote and wild-type. Also resistant to methoprene induced morphogenetic abnormalities and tumours.

(Wilson and Fabian, 1986)

Semidominant mutation conferring 50-100 fold increase in resistance to juvenile hormone III or its analogue methoprene over that of wild type. Also resistant to methoprene-induced tumors and abnormalities in adult cuticle; action of Rst(1)JH autonomous in gynandromorphs. Wilson speculates that gene may affect juvenile-hormone receptor.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Met¹ is a non-enhancer of abnormal chemosensory behavior | adult stage | male phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Met¹ is a non-enhancer of abnormal neurophysiology | adult stage | male phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of abnormal cell migration | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

NOT Suppressor of

Statement

Reference

Met¹ is a non-suppressor of abnormal chemosensory behavior | male | adult stage phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Met¹ is a non-suppressor of abnormal neurophysiology | male | adult stage phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has partially lethal - majority die phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has decreased fecundity phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has abnormal cell migration | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Met¹, gce^MI02742 has decreased fecundity phenotype

(Barton et al., 2024)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Met¹ is a non-enhancer of adult olfactory receptor neuron Or47b | male phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of primordial germ cell | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

NOT Suppressor of

Statement

Reference

Met¹ is a non-suppressor of adult olfactory receptor neuron Or47b | male phenotype of Scer\GAL4^Or47b.7.467, fru^UAS.MB

(Zhang et al., 2020)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has primordial germ cell | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

3472

107055

Notes on Origin

Discoverer

Comments

Analysis of gynandromorphs shows that Rst(1)JH¹ acts autonomously.

(Wilson and Fabian, 1986)

The fitness component(s) responsible for the competitive disadvantage of flies carrying mutant alleles of Rst(1)JH have been analysed in Rst(1)JH¹, Rst(1)JH², Rst(1)JH³, Rst(1)JH^D29 and Rst(1)JH^N6 flies. Small but significant differences were found between the pooled Rst(1)JH alleles and wild-type for pupal developmental time, pupal mortality, and early adult fecundity. These differences results in a large competitive disadvantage.

(Minkoff and Wilson, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Met

(Wilson, 1996, Shemshedini and Wilson, 1990, Shemshedini et al., 1990)

Met1

(Wilson and Fabian, 1986)

Met¹

(Barton et al., 2024, Leinwand and Scott, 2021, Zhang et al., 2020, Kang et al., 2017, Wilson et al., 2006, Beno et al., 2005, Flatt and Kawecki, 2004, Ashok et al., 1998, Restifo and Wilson, 1998, Riddiford and Ashburner, 1991)

Rst(1)JH¹

Name Synonyms

Secondary FlyBase IDs

References (15)

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