FlyBase Allele Report: Dmel\Scr[2]

General Information

Symbol

Dmel\Scr²

Species

D. melanogaster

Name

FlyBase ID

FBal0015278

Feature type

allele

Associated gene

Dmel\Scr

Associated Insertion(s)

Carried in Construct

Also Known As

Scr^k6, EdK6, Scr^eK6, Scr^EdK6

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Sivanantharajah and Percival-Smith, 2009, Pattatucci and Kaufman, 1991, Burgess and Duncan, 1990, Reuter et al., 1990)

loss of function allele

(Pattatucci et al., 1991)

Mutagen

X ray

(Pattatucci et al., 1991)

ethyl methanesulfonate

(Lewis et al., 1980, Lewis et al., 1980)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Pattatucci et al., 1991)

Description

Nucleotide substitution: C340T.

(Sivanantharajah and Percival-Smith, 2009)

Amino acid replacement: Q114term.

(Sivanantharajah and Percival-Smith, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,841,841..6,841,841 [-]

Nucleotide change:

C6841841T

Reported nucleotide change:

C340T

Amino acid change:

Reported amino acid change:

Q114term

(Sivanantharajah and Percival-Smith, 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Lewis et al., 1980, Lewis et al., 1980)

visible

(Percival-Smith et al., 1997)

visible (with Scr^Scx)

(Southworth and Kennison, 2002)

visible | dominant

(Sivanantharajah and Percival-Smith, 2009, Southworth and Kennison, 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

The head structures are severely disrupted in first instar homozygous larvae and the number of rows of T1 beard denticles is reduced.

Heterozygous males have a reduction in the number of sex comb bristles on the prothoracic leg compared to wild-type males.

(Sivanantharajah and Percival-Smith, 2009)

Heterozygous males have a reduced number of sex comb teeth on the first leg (average is 6.3 per leg in these animals). Heterozygous males have no ectopic sex comb teeth on the second or third legs. Scr^Scx/Scr² males have an average of 1.3 ectopic sex comb teeth per second leg and no ectopic sex comb teeth on the third legs.

(Southworth and Kennison, 2002)

pb²⁷ Scr² mosaic clones in the proboscis transform to an aristae and to a third antennal segment with second antennal segment bristle. Clones in the tarsus never exhibit transformation. Clones of pb²⁷ Scr² cells in the ectoderm (proboscis precursor cells) of pb²⁷/pb²⁰ mutants adopt tarsal identity and not aristal identity as in a pb⁺ organism. Mitotic clones in the tarsi of wild type do not exhibit transformation to aristae.

(Percival-Smith et al., 1997)

Salivary glands are absent at all stages of development.

(Panzer et al., 1992)

Haplo-abnormal phenotype is suppressed by a duplication carrying Scr.

(Kennison and Russell, 1987)

Heterozygous males exhibit a reduction in the number of sex comb teeth, indicating a homeotic transformation of prothoracic to mesothoracic leg identity.

(Lewis et al., 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scr², hth^5E04, pb²⁷, put^p1 has visible | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has visible | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has visible | somatic clone phenotype

(Joulia et al., 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scr² has sex comb phenotype, non-enhanceable by Pcl¹⁰

(Southworth and Kennison, 2002)

Scr² has sex comb phenotype, non-enhanceable by Pc⁴

(Southworth and Kennison, 2002)

NOT suppressed by

Statement

Reference

Scr² has sex comb phenotype, non-suppressible by Pc⁴

(Southworth and Kennison, 2002)

Scr² has sex comb phenotype, non-suppressible by Pcl¹⁰

(Southworth and Kennison, 2002)

Other

Statement

Reference

Scr², hth^5E04, pb²⁷, put^p1 has labellum | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1 has arista | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has labellum | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has antenna | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has unguis | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has labellum | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has bract | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has arista | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², exd¹, pb²⁷ has arista phenotype

(Percival-Smith and Hayden, 1998)

Scr², exd¹, pb²⁷ has antenna phenotype

(Percival-Smith and Hayden, 1998)

Scr², exd¹, pb²⁷ has proboscis phenotype

(Percival-Smith and Hayden, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

pb²⁷ Scr² hth^5E04 clones in the labium result in transformation of the labium to a compact leg appendage identified by claws and bracted bristles in 81% of cases, while in the remaining 19% of cases the transformed structure contains a mix of leg and antennal tissue.

pb²⁷ Scr² hth^5E04 put^p1 clones in the labium show transformation to antennal aristae in 76% of cases and mixed transformations are not seen.

pb²⁷ Scr² hth^5E04 put^p1 ss^a clones in the labium show a fully penetrant transformation to stunted legs.

(Joulia et al., 2006)

The average number of sex comb teeth per first leg Scr²/+ males is not affected if they are also carrying Pc⁴ or Pcl¹⁰.

(Southworth and Kennison, 2002)

Clones triple mutant for pb²⁷, Scr² and exd¹ generated 30-60 hours after egg laying show transformations of arista to tarsus, antenna to second leg and rostrum to ectopic eye, with associated leg. Both pb²⁷ and pb²⁷, Scr² clones of ectodermal proboscis cells adopt aristal identity. However, both pb²⁷, exd¹ and pb²⁷, Scr², exd¹ clones of ectodermal proboscis cells adopt tarsal (second leg) identity. Clones of pb²⁷ cells in the proboscis form sex combs. Clones of pb²⁷, exd¹ cells in the proboscis are transformed to tarsal identity and in addition they form sex combs. When the clones are of the genotype pb²⁷, Scr², exd¹, no sex combs form.

(Percival-Smith and Hayden, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2185

Scr² p^p cu¹/TM6B, Tb¹

Kyoto

107168

Dp(3;1)P115; Df(3R)P9, st¹ in¹ kni^ri-1 Pc³ Scr² p^p/TM3, Sb¹

Notes on Origin

Discoverer

Kaufman.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

EdK6

(Lewis et al., 1980, Lewis et al., 1980)

Scr2

(Jordan et al., 2002)

Scr²

(Feng et al., 2022, Kuert et al., 2014, Roper, 2012, Sivanantharajah and Percival-Smith, 2009, Joulia et al., 2006)

Scr^EdK6

(Shroff et al., 2007, Kennison and Russell, 1987)

Scr^K6

(Burgess and Duncan, 1990, Kennison and Russell, 1987, Sato and Denell, 1985)

Scr^eK6

(Panzer et al., 1992)

Scr^ek6

(Harris and Beckendorf, 2007)

Scr^k6

(Pattatucci et al., 1991, Reuter et al., 1990, Kennison and Russell, 1987, Hazelrigg and Kaufman, 1983)

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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