FlyBase Allele Report: Dmel\sn[36a]

General Information

Symbol

Dmel\sn^36a

Species

D. melanogaster

Name

FlyBase ID

FBal0015801

Feature type

allele

Associated gene

Dmel\sn

Associated Insertion(s)

?{}sn^36a

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Borghese et al., 2006)

Mutagen

spontaneous

(Cant et al., 1994, Roiha et al., 1988)

Progenitor genotype

Associated Insertion(s)

?{}sn^36a

Cytology

Description

Insertion in the 5' untranslated region.

(Cant et al., 1994)

5.5kb insertion between -1.2 and 0.0 (coordinates as in FBrf0048245).

(Paterson and O'Hare, 1991)

Insertion of 5.5kb DNA, at coordinates -1.2 to 0.0.

(Roiha et al., 1988)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Nagel et al., 2012)

female sterile

(Roiha et al., 1988)

sterile

(Cant et al., 1994)

Phenotype Manifest In

larval thoracic lateral multidendritic neuron ldaB | third instar larval stage

(Nagel et al., 2012)

macrochaeta

(Cant et al., 1994, Roiha et al., 1988)

Detailed Description

Statement

Reference

The number and density of spiked protrusions on the dendrites of class III ldaB neurons is reduced in mutant third instar larvae compared to wild type, while the total branch length of the dendritic tree is normal. The neuron terminal branchlets are bent in the mutant larvae, in contrast to wild type. The fraction of spiked protrusions that exceed 10 μm in length is increased in the mutant larvae. The complexity of the dendritic tree appears slightly increased (for example, the number of all termini excluding the terminal branchlets of less than 30 μm is significantly increased). The number of spiked protrusions on the class III neuron dendrites is also reduced in mutant second instar larvae, and a reduced rate of formation of new branchlets is seen at this stage compared to wild type.

(Nagel et al., 2012)

Homozygous border cell clones show normal migration.

(Borghese et al., 2006)

Bristles are gnarled due to disrupted actin filament bundles. Nurse cell cytoplasmic actin filaments are absent.

(Cant et al., 1994)

Extreme bristle phenotype, female sterile.

(Roiha et al., 1988)

Macrochaetae gnarled in a fairly extreme manner. Microchaetae wild type. sn^36a is only allele to cause pronounced reduction in replication of oocyte nurse cell DNA (King and Burnett, 1957). Also causes more extreme retardation of vitellogenesis than other female-sterile sn alleles (Bender, 1960). sn^36a sn⁴ homozygote has nearly normal bristles and is sterile. RK1. class 4 - female sterile with gnarled macrochaetae only

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

sn^36a has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ct^Scer\UAS.cPa under the control of Scer\GAL4²²¹ in a sn^36a background in class I vpda neurons results in an expansion of the dendrite tree. In addition, the formation of ectopic spiked protrusions is reduced by more than 40% compared to the expression of ct^Scer\UAS.cPa under the control of Scer\GAL4²²¹ in a wild-type background.

(Nagel et al., 2012)

Homozygous sn^36a border cell clones in a qua¹/+ background show normal migration.

Homozygous qua¹ border cell clones in a sn^36a/+ background show normal migration.

(Borghese et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sn^36a is rescued by Scer\GAL4^smid-C161/sn^UAS.mKO

(Nagel et al., 2012)

sn^36a is rescued by sn^S52A.UAS.mKO/Scer\GAL4^smid-C161

(Nagel et al., 2012)

sn^36a is rescued by Scer\GAL4^smid-C161/sn^S52D.UAS.mKO

(Nagel et al., 2012)

Comments

Expression of sn^{Scer\UAS.T:Zzzz\KO-mKO} under the control of Scer\GAL4^smid-C161 rescues the reduction in number and density of spiked protrusions on the dendrites of class III ldaB neurons that is seen in sn^36a third instar larvae.

Expression of sn^{S52A.Scer\UAS.T:Zzzz\KO-mKO} or sn^{S52D.Scer\UAS.T:Zzzz\KO-mKO} under the control of Scer\GAL4^smid-C161 rescues the reduction in density of spiked protrusions on the dendrites of class III ldaB neurons that is seen in sn^36a third instar larvae.

(Nagel et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

117

sn^36a/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Spencer, 21st Jan. 1936.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sn^36a

(Yoong et al., 2020, Groen et al., 2015, Nagel et al., 2012, Schottenfeld-Roames and Ghabrial, 2012, Borghese et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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