FlyBase Allele Report: Dmel\spi[2]

General Information

Symbol

Dmel\spi²

Species

D. melanogaster

Name

FlyBase ID

FBal0016006

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

Also Known As

spi^IIT25, spi^T25

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Kerber et al., 1998)

Mutagen

ethyl methanesulfonate

(Rutledge et al., 1992, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Gonzalez-Gaitan and Jackle, 2000)

lethal - all die during embryonic stage

(Huang et al., 1998)

some die during embryonic stage

(Huang et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

spi² homozygous embryos exhibit a mild invagination phenotype (approximately 35% of the placodes show an abnormal invagination).

(Brodu and Casanova, 2006)

In spi² stage 15 embryos, the genital disc precursor cells are completely missing.

(Chen et al., 2005)

spi² mutant embryos have about 23 cells in each Malpighian tubule cell (as opposed to about 125 in wild-type).

(Sudarsan et al., 2002)

The stomatogastric nervous system (SNS) anlage forms normally in mutant embryos. However, the stomodeal invaginations that are seen in wild-type stage 11 embryos are not seen in mutant embryos at this stage. Stage 17 mutant embryos lack SNS ganglia and nerves.

(Gonzalez-Gaitan and Jackle, 2000)

Mutant somatic clones have no effect on the lamina. Clones in the eye disc contain only isolated R8 neurons, which project to appropriate retinotopic positions in the lamina, and trigger early LPC maturation but not their neuronal differentiation.

(Huang et al., 1998)

Homozygous embryos have a reduced number of cells in the anterior Malpighian tubules compared to wild-type embryos.

(Kerber et al., 1998)

Germline clones fail to rescue the female sterile phenotype of Fs(2)Ugr.

(Sapir et al., 1998)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Epidermal defects and fusion of the anterior and posterior commissures. In the PNS some sensory organs are missing, there is a ventral-dorsal gradient of severity. Several muscle fibres are always missing in the dorsolateral region and there are variable abnormalities in the number, shape and attachment sites of the eight ventral oblique muscle fibres.

(Rutledge et al., 1992)

embryonic lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

spi² has tracheal primordium phenotype, enhanceable by cv-c[+]/cv-c⁷

(Brodu and Casanova, 2006)

spi² has presumptive embryonic/larval tracheal system phenotype, enhanceable by cv-c[+]/cv-c⁷

(Brodu and Casanova, 2006)

Suppressor of

Statement

Reference

spi² is a suppressor of phenotype of rho^hs.sev

(Freeman, 1994)

Additional Comments

Genetic Interactions

Statement

Reference

The mild invagination observed in spi² homozygous embryos is significantly enhanced by the presence of a single copy of cv-c⁷, with approximately 67% of placodes exhibiting an abnormal invagination, compared to approximately 35% in spi² mutants.

(Brodu and Casanova, 2006)

Reduction in dose of spi rescues the rho^hs.sev eye to a near wild-type phenotype; regular array of ommatidia, regular arrangement of photoreceptors and unbroken lattice of pigment cells. Rescue is not always complete.

(Freeman, 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spi^SCP2/spi² is rescued by Scer\GAL4^GMR.PF/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi² is rescued by Scer\GAL4^αTub84B.PP/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

Partially rescued by

spi^SCP2/spi² is partially rescued by spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

Comments

Animals heterozygous for spi^SCP2 and spi² which have been rescued by spi^Scer\UAS.cSa with Scer\GAL4^hs.PB to the third instar stage show relatively normal ommatidial development at the posterior of the eye imaginal disc. More anterior columns of ommatidia have reduced photoreceptors. In the lamina, retinal projections appeared normal but LPCs show no neuronal differentiation. This phenotype is rescued by the introduction of Scer\GAL4^GMR.PF or in clones induced to produce Scer\GAL4^αTub84B.PP.

(Huang et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34508

spi² cn¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

spi²

(Brodu and Casanova, 2006)

spi^IIT25

(Chen et al., 2005, Sudarsan et al., 2002, Kerber et al., 1998, Sapir et al., 1998, Golembo et al., 1996, Golembo et al., 1996, Raz and Shilo, 1993, Rutledge et al., 1992)

spi^IIT

(Tearle and Nusslein-Volhard, 1987)

spi^T25

(Huang et al., 1998, Huang and Fischer-Vize, 1996, Schweitzer et al., 1995, Freeman, 1994)

spitz^IIT

(Gonzalez-Gaitan and Jackle, 2000)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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