FlyBase Allele Report: Dmel\stl[4]

General Information

Symbol

Dmel\stl⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0016197

Feature type

allele

Associated gene

Dmel\stl

Associated Insertion(s)

Carried in Construct

Also Known As

stl^PH57

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement (Q to stop) at a position that corresponds to residue 520 in the A isoform, residue 821 in the B isoform, and residue 866 in the C isoform.

(Ozdowski et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:22,814,838..22,814,838 [+]

Nucleotide change:

C22814838T

Amino acid change:

Q866term | stl-PC; Q866term | stl-PE

Reported amino acid change:

Q866term

Reported_genomic_loc:

2R_r5:18702343..18702343

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The amino acid sequence changes were reported with respect to three alternative transcripts.

(Ozdowski et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage

(Lhamo and Ismat, 2015)

abnormal cell migration | embryonic stage (with stl²)

(Lhamo and Ismat, 2015)

abnormal cell migration | embryonic stage (with stl³)

(Lhamo and Ismat, 2015)

decreased cell number | oogenesis

(Willard et al., 2004)

female sterile

(Schupbach and Wieschaus, 1991)

Phenotype Manifest In

abdominal lch1 neuron | embryonic stage

(Lhamo and Ismat, 2015)

abdominal lch1 neuron | embryonic stage (with stl²)

(Lhamo and Ismat, 2015)

abdominal lch1 neuron | embryonic stage (with stl³)

(Lhamo and Ismat, 2015)

basal stalk

(Tworoger et al., 1999)

germarium

(Tworoger et al., 1999)

germline cyst

(Tworoger et al., 1999)

ovariole

(Willard et al., 2004)

ovary (with stl³)

(Ozdowski et al., 2009)

stalk follicle cell

(Willard et al., 2004)

Detailed Description

Statement

Reference

stl⁴, stl²/stl⁴ and stl³/stl⁴ mutant embryos display significantly faster dorsal migration of the v'ch1 sensory neuron during stages 15-16, to its target the lch5 chordotonal organ cluster, as compared to wild type, and no over-migration past its target.

(Lhamo and Ismat, 2015)

Ovaries of 10 day old stl³/stl⁴ females show severe degeneration.

(Ozdowski et al., 2009)

Mutant ovarioles in adult females lack the interfollicular stalks that normally separate adjacent wild-type follicles. This phenotype results from failed follicle individualisation, rather than from persistent germline cell division, because mutant ovarioles contain germ cells at varying stages of maturation and groups of 16 interconnected germ cells that would correspond to a single germline cyst in wild-type are still recognisable. Degeneration is seen in the mutant ovarioles, which increases with age, and both germline and somatic cells appear to degenerate.

(Willard et al., 2004)

Germline cysts accumulate in an enlarged germarium in stl⁴/stl³ females. stl⁴/stl² females lack morphological stalks in the ovary.

(Tworoger et al., 1999)

Has yolk containing cells at various locations within the egg chambers, many egg chambers degenerate, few collapsing eggs. Males are fertile.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Delta³, stl[+]/stl⁴ has ovariole phenotype

(Ozdowski et al., 2009)

Delta⁹, stl[+]/stl⁴ has ovariole phenotype

(Ozdowski et al., 2009)

Delta^X, stl[+]/stl⁴ has ovariole phenotype

(Ozdowski et al., 2009)

+/Df(3R)Delta-BX12, stl⁴ has ovariole phenotype

(Ozdowski et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

95.4% of stl⁴/+ ; Dl⁹/+ ovarioles have compound or morphologically disrupted follicles.

96.1% of stl⁴/+ ; Dl³/+ ovarioles have compound or morphologically disrupted follicles.

58.3% of stl⁴/+ ; Dl^X/+ ovarioles have compound or morphologically disrupted follicles.

20.8% of stl⁴/+ ; Df(3R)Dl-BX12/+ ovarioles have compound or morphologically disrupted follicles.

(Ozdowski et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PH57

(Tworoger et al., 1999)

stl⁴

stl^PH57

(Willard et al., 2004, Schupbach and Wieschaus, 1991)

stl^PH

stl^ph57

(Lhamo and Ismat, 2015, Ozdowski et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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