FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ace+mFa
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General Information
Symbol
Dmel\Ace+mFa
Species
D. melanogaster
Name
FlyBase ID
FBal0028716
Feature type
allele
Associated gene
Dmel\Ace
Associated Insertion(s)
Carried in Construct
P{Ace.F}
P{Ace.Fb}
Key Links
Transgenic product class
wild_type
(Fournier et al., 1992)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Fournier et al., 1992)
Mutagen
in vitro construct
(Incardona and Rosenberry, 1996, Fournier et al., 1992)
Progenitor genotype
Carried in construct
P{Ace.F}
P{Ace.Fb}
Cytology
Description

5.8kb Ace minigene consisting of 1.5kb 5' genomic DNA from the Ace region fused to a full length Ace cDNA.

(Incardona and Rosenberry, 1996)

Intronless Ace coding region fused to 1.5 kb of promoter sequences.

(Fournier et al., 1992)

Gene size reduced from 34kb to 5.8 kb. Heat shock terminal sequence ligated to 3' end cDNA.

(Hoffmann et al., 1992)
Allele components
Component
Use(s)
Regulatory region(s)
Ace
Encoded product / tool
Ace
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Abl1 NrtM2 double mutant causes absence of most intersegmental longitudinal axon bundles and most commissural axon bundles. The loss of pros has an additive effect on the mutant phenotype. Loss of Abl function does not detectably alter pros mutant phenotype.

      (Incardona and Rosenberry, 1996)

      The minigene can successfully rescue Ace- lethality. In rescued flies all acetylcholinesterase activity is derived from the minigene. They display only 20-25% of wild type activity, and low levels of resistance to malathion.

      (Fournier et al., 1992)

      Reduced viability with respect to wild type due to low enzyme activity.

      (Hoffmann et al., 1992)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Wild type flies transformed with the minigene construct have 120--130% of wild type enzyme activity and high levels of resistance to malathion.

      (Fournier et al., 1992)

      The minigene provides only 20--30% of wild type activity, close to the threshold level for survival.

      (Hoffmann et al., 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ace+mFa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)