FlyBase Allele Report: Dmel\Ras64B[Val14.UAS]

General Information

Symbol

Dmel\Ras64B^Val14.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0030096

Feature type

allele

Associated gene

Dmel\Ras64B

Associated Insertion(s)

Carried in Construct

P{UAS-Ras64B.V14}

Key Links

Transgenic product class

missense_variant

(Brand and Perrimon, 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Brand and Perrimon, 1993)

Mutagen

in vitro construct

(Brand, 1997.6.30, Brand and Perrimon, 1993)

Progenitor genotype

Carried in construct

P{UAS-Ras64B.V14}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of Ras64B that is predicted to produce a constitutively active protein (carries the amino acid replacement G14V).

(Brand and Perrimon, 1993)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ras64B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA with HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa

(Padash Barmchi et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage, with Scer\GAL4^Ras64B.PW

(Walker et al., 2006)

lethal, with Scer\GAL4^69B

(Brand and Perrimon, 1993)

lethal - all die before end of pupal stage, with Scer\GAL4^h-1J3

(Brand and Perrimon, 1993)

lethal - all die before end of pupal stage, with Scer\GAL4^how-24B

(Brand and Perrimon, 1993)

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^Ras64B.PW

(Walker et al., 2006)

some die during pupal stage, with Scer\GAL4^h-1J3

(Brand and Perrimon, 1993)

some die during pupal stage, with Scer\GAL4^how-24B

(Brand and Perrimon, 1993)

some die during pupal stage | heat sensitive, with Scer\GAL4^Ras64B.PW

(Walker et al., 2006)

visible, with Scer\GAL4^30A

(Brand and Perrimon, 1993)

visible, with Scer\GAL4^32B

(Brand and Perrimon, 1993)

visible, with Scer\GAL4^71B

(Brand and Perrimon, 1993)

visible, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

visible, with Scer\GAL4^hs.2sev

(Brand and Perrimon, 1993)

Phenotype Manifest In

eye, with Scer\GAL4^30A

(Brand and Perrimon, 1993)

eye, with Scer\GAL4^32B

(Brand and Perrimon, 1993)

eye, with Scer\GAL4^71B

(Brand and Perrimon, 1993)

eye, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

eye, with Scer\GAL4^hs.2sev

(Brand and Perrimon, 1993)

ommatidium, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

wing, with Scer\GAL4^30A

(Brand and Perrimon, 1993)

wing, with Scer\GAL4^32B

(Brand and Perrimon, 1993)

wing, with Scer\GAL4^71B

(Brand and Perrimon, 1993)

Detailed Description

Statement

Reference

Expression of Ras64B^{Val14.Scer\UAS} under the control of Scer\GAL4^Ras64B.PW results in small dead pupae at 25 or 18^oC whereas at 16^oC, adult flies that are smaller than normal are produced.

(Walker et al., 2006)

Expression of Ras64B^{Val14.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in extreme overgrowth of the eye, lack of ommatidial organisation and the formation of huge ommatidia.

(Kramer et al., 2003)

When Ras85D^N17.Scer\UAS is driven by Scer\GAL4^ftz.ng, no midline crossovers are seen in the pCC/MP2 pathway axons.

(Fritz and VanBerkum, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^NP1301/Ras64B^Val14.UAS is a suppressor of abnormal neuroanatomy phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Ras64B^Val14.UAS, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Yang and Terman, 2012)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Mmus\Foxo1^UAS.cKa, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

NOT Suppressor of

Statement

Reference

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^NP1301/Ras64B^Val14.UAS is a suppressor of larval segmental nerve branch SNa of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Ras64B^Val14.UAS, Scer\GAL4^elav.PU is a suppressor of larval central nervous system phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Yang and Terman, 2012)

Scer\GAL4^NP1301/Ras64B^Val14.UAS is a suppressor of larval intersegmental nerve branch ISNb of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Mmus\Foxo1^UAS.cKa, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^dpp.3KK/Ras64B^Val14.UAS is a non-suppressor of wing vein phenotype of Mkp3^EP-M76

(Ruiz-Gomez et al., 2005)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a non-suppressor of interommatidial bristle phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Ras64B^Val14.UAS, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The ISNb and SNa axon guidance defects seen in 14-3-3ε^j2B10/14-3-3ε^NP1301 embryos are significantly suppressed by expression of Ras64B^{Val14.Scer\UAS} under the control of Scer\GAL4^{14-3-3ε-NP1301}.

(Yang and Terman, 2012)

Expression of either Ras64B^{Val14.Scer\UAS} does not affect the wing vein phenotype of Scer\GAL4^dpp.3KK>Mkp3^GSM76 flies.

(Ruiz-Gomez et al., 2005)

Co-expression of Ras64B^{Val14.Scer\UAS} is sufficient to restore many of the ommatidia and mechanosensory bristles that are lost in the eyes of animals expressing foxo^Scer\UAS.cKa.

(Kramer et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2025