FlyBase Allele Report: Dmel\faf[B3]

General Information

Symbol

Dmel\faf^B3

Species

D. melanogaster

Name

FlyBase ID

FBal0031239

Feature type

allele

Associated gene

Dmel\faf

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Fischer-Vize et al., 1992)

Mutagen

ethyl methanesulfonate

(Fischer-Vize et al., 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q71term.

(Chen and Fischer, 2000)

Nucleotide substitution: C?T.

(Chen and Fischer, 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,778,676..31,778,676 [+]

Nucleotide change:

C31778676T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q71term

(Chen and Fischer, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by pk^sple-1

(Paemka et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Fischer-Vize et al., 1992)

partially lethal - majority die (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

partially lethal - majority die (with faf^FO8)

(Stinchfield et al., 2012)

visible | recessive

(Fischer-Vize et al., 1992)

Phenotype Manifest In

denticle (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

denticle (with faf^FO8)

(Stinchfield et al., 2012)

embryonic/first instar larval cuticle (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

embryonic/first instar larval cuticle (with faf^FO8)

(Stinchfield et al., 2012)

embryonic/first instar larval cuticle | non-rescuable maternal effect (with faf^B5)

(Stinchfield et al., 2012)

embryonic head (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

filzkorper (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

filzkorper (with faf^FO8)

(Stinchfield et al., 2012)

primordial germ cell

(Liu et al., 2003)

ventral denticle belt | ectopic (with faf^FO8)

(Stinchfield et al., 2012)

ventral denticle belt | ectopic | non-rescuable maternal effect (with faf^B5)

(Stinchfield et al., 2012)

Detailed Description

Statement

Reference

Only 27% of the expected number of faf^FO8/faf^B3 zygotic mutants survive. The embryos have a ventralised cuticle with dorsally extended denticles and an ectopic denticle belt replacing the Filzkorper.

faf^B3/Df(3R)faf-BP embryos have a ventralised cuticle with herniated head, dorsally extended denticles and an misplaced Filzkorper.

Embryos derived from faf^B3/faf^B5 females mated to wild-type males show almost complete ventralisation of the cuticle. The rudimentary cuticle contains one anterior denticle belt that fully encircles the embryo.

(Stinchfield et al., 2012)

Only 7% of embryos derived from faf^B3 females form pole cells, and these embryos have far fewer pole cells than in wild-type embryos. The pole cells that form show a variety of migration defects, failing to migrate into the abdomen and instead spreading out over the embryo surface. Many of the embryos fail to undergo or undergo abnormal nuclear divisions; 45% of embryos derived from faf^B3 females are unfertilised (one nucleus), 12% are arrested at a two nuclear stage and 40% are multinucleated, but usually have irregularly arranged nuclei.

(Liu et al., 2003)

Eyes roughened due to abnormalities in the hexagonal facet array: most facets include 1-3 extra outer photoreceptor cells which are probably mystery cells. Ovaries of homozygous females are normal in appearance but the embryos produced never form syncytial blastoderm and all fail to hatch.

(Fischer-Vize et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

faf[+]/faf^B3 is a suppressor of bang sensitive | dominant phenotype of pk^sple-1

(Paemka et al., 2015)

Other

Statement

Reference

Med⁸, faf[+]/faf^B3 has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The presence of faf^B3/+ rescues the bang-sensitive phenotype of pk^sple-1/+ mutants.

(Paemka et al., 2015)

Embryos derived from Med⁸/+ females mated to males heterozygous for faf^B3 show semi-lethality.

(Stinchfield et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (1)

Bloomington

25100

st¹ faf^B3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

faf^B3

(Dawson et al., 2025, Paemka et al., 2015, Stinchfield et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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