FlyBase Allele Report: Dmel\Med[8]

General Information

Symbol

Dmel\Med⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0090583

Feature type

allele

Associated gene

Dmel\Med

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Yoshida et al., 2005)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W728term.

(Wisotzkey et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,614,768..31,614,768 [+]

Nucleotide change:

G31614768A

Amino acid change:

W728term | Med-PA; W654term | Med-PB; W728term | Med-PC

Reported amino acid change:

W728term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Wisotzkey et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle

(Wisotzkey et al., 1998)

eye disc morphogenetic furrow | somatic clone

(Baonza and Freeman, 2001)

eye photoreceptor cell stalk | somatic clone

(Yoshida et al., 2005)

lamina | somatic clone

(Yoshida et al., 2005)

wing disc | somatic clone

(Wisotzkey et al., 1998)

Detailed Description

Statement

Reference

Med⁸ mutants exhibit defects in R axon projection and lamina morphology. Such defects are only observed when large clones are generated in the posterior-dorsal or ventral domains, which presumably include glial cell progenitors. Clones in other regions, such as the outer proliferation center, lamina or medulla, do not result in R-axon targeting defects.

(Yoshida et al., 2005)

Clones in the eye disc prevent initiation of the morphogenetic furrow when they are at the posterior margin of the disc.

(Baonza and Freeman, 2001)

Females with homozygous germ line clones produce eggs that can be fertilised. Homozygous embryos derived from females with homozygous germ line clones have fully ventralised cuticles. Heterozygous embryos derived from females with homozygous germ line clones have a partially ventralised phenotype; some dorsal tissue lacking denticle belts differentiates, but head involution does not occur. Homozygous clones in the wing pouch do not survive when induced during the first larval instar stage, although clones are recovered elsewhere in the wing disk. Homozygous clones of variable size are recovered in the wing pouch when induced in the middle of the second larval instar stage (66 hours before pupariation).

(Wisotzkey et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Med⁸, dpp^hr4/dpp[+] has lethal | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Med⁸, faf[+]/faf^B3 has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Med⁸, faf[+]/faf^B4 has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Df(3R)faf-BP/+, Med⁸ has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Med⁸, faf^FO8/faf[+] has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

Med⁸, dpp^hr4/dpp[+] has embryonic/first instar larval cuticle | maternal effect phenotype

(Stinchfield et al., 2012)

Med⁸, faf[+]/faf^B4 has embryonic/first instar larval cuticle | maternal effect phenotype

(Stinchfield et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Animals derived from Med⁸/+ females mated to dpp^hr4/+ males show strong lethality (5% of the expected number survive). The embryos have a ventralised cuticle phenotype.

Embryos derived from Med⁸/+ females mated to males heterozygous for one of faf^B3, Df(3R)faf-BP or faf^FO8 show semi-lethality.

Embryos derived from Med⁸/+ females mated to males heterozygous for faf^B4 show semi-lethality. The embryos have a ventralised cuticle.

Embryos derived from faf^B4/+ females mated to Med⁸/+ males have a wild-type cuticle.

(Stinchfield et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The survival of the progeny of Med⁸/+ females mated to dpp^hr4/+ males is increased 1.6-fold by basal expression of Med^{Scer\UAS.P\T.cSa} (in the absence of a Scer\GAL4 driver) in the females, while basal expression of Med^{K738R.Scer\UAS.P\T} in the females increases survival of the progeny 3.6-fold. Expression of either Med^{Scer\UAS.P\T.cSa} or Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females fully rescues the lethality of the embryos.

(Stinchfield et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Med⁸

(Stinchfield et al., 2012, Quijano et al., 2011)

med⁸

(Baonza and Freeman, 2001)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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