FlyBase Allele Report: Dmel\mago[1]

General Information

Symbol

Dmel\mago¹

Species

D. melanogaster

Name

FlyBase ID

FBal0032138

Feature type

allele

Associated gene

Dmel\mago

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

heat sensitive antimorphic allele - genetic evidence

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

heat sensitive antimorphic allele - genetic evidence

(Boswell et al., 1991)

hypomorphic allele - genetic evidence

(Boswell et al., 1991)

Mutagen

ethyl nitrosourea

(Boswell et al., 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: G19R.

(Newmark et al., 1997, Newmark and Boswell, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,128,748..21,128,748 [+]

Nucleotide change:

G21128748A

Amino acid change:

G19R | mago-PA

(Newmark and Boswell, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage

(Micklem et al., 1997, Newmark et al., 1997)

egg

(Micklem et al., 1997, Newmark et al., 1997)

egg | germline clone

(Newmark et al., 1997)

egg chamber | germline clone

(Newmark et al., 1997)

egg operculum

(Micklem et al., 1997)

micropyle

(Micklem et al., 1997, Newmark et al., 1997)

microtubule & egg chamber

(Newmark et al., 1997)

microtubule & oocyte

(Micklem et al., 1997)

oocyte nucleus

(Micklem et al., 1997, Newmark et al., 1997)

oocyte nucleus | germline clone

(Newmark et al., 1997)

posterior aeropyle

(Micklem et al., 1997)

Detailed Description

Statement

Reference

Ooplasmic streaming is normal at stage 9 and stage 10b in mago¹/Df(2R)F36 oocytes.

(Palacios and St. Johnston, 2002)

The nucleus fails to migrate to the anterior of the oocyte in approximately 30% of the egg chambers of mago¹/Df(2R)F36 females at 18^oC. The penetrance of this phenotype is less than 3% at 25^oC. The nucleus also fails to migrate to the anterior of the oocyte in some of the egg chambers of mago¹/mago¹ and mago¹/mago² females. Stage 9 mago¹/Df(2R)F36 egg chambers have an abnormal distribution of microtubules at 18^oC, with high levels of microtubules at both poles of the oocyte and lower levels in the centre. Microtubule organisation appears more normal in later stage egg chambers at 18^oC, although a small posterior focus of microtubules often persists until stage 10-11. Microtubule organisation appears more normal in mago¹/Df(2R)F36 egg chambers at 25^oC, although a small focus of microtubules is seen at the posterior pole during stages 8-9. Approximately 30% of eggs laid by mago¹/Df(2R)F36 females are symmetrical along the dorsal-ventral axis. These eggs are completely ventralised at the anterior end; they lack dorsal appendages, the operculum develops as a symmetric ring around the micropyle and the micropyle points anteriorly rather than dorsally. The egg shell is more pointed at the posterior pole than normal, and the posterior aeropyle is larger and more symmetric than wild-type.

(Micklem et al., 1997)

Approximately 1% of eggs derived from hemizygous females raised at 25^oC are ventralised. Approximately 38% of eggs derived from hemizygous females shifted from 25^oC to 17^oC are ventralised. The dorsal appendages are missing in these ventralised eggs and the micropyle canal is defective, although the aeropyle appears normal. Approximately 1% of eggs derived from hemizygous females raised at 25^oC contain a mislocalised oocyte nucleus. Approximately 36% of eggs derived from hemizygous females shifted from 25^oC to 17^oC contain a mislocalised oocyte nucleus. Homozygous female germline clones also give rise to egg chambers containing mislocalised oocyte nuclei and ventralised eggs. The polarisation of the microtubule cytoskeleton is abnormal in hemizygous egg chambers shifted from 25^oC to 17^oC.

(Newmark et al., 1997)

Prevents the posterior accumulation of G-iα65A protein.

(Wolfgang and Forte, 1995)

Offspring fail to make pole cells. Shows temperature sensitive defects in abdominal segmentation and embryonic body plan.

(Newmark and Boswell, 1994)

When mutant females are raised at 25^oC some of the resulting embryos have double abdomens. At 17^oC the females produce embryos displaying abdominal defects typical of the posterior group, with no incidence of head defects or double abdomens.

(Bardsley et al., 1993)

Grandchildless-like phenotype. Eggs from homozygous females have no apparent posterior determinants in the posterior pole. At 17^oC the inviable embryos lack abdominal segments, at 25^oC a novel gene product is produced that is capable of inducing the formation of symmetrical double abdomen embryos (behave as recessive antimorphs). The temperature sensitive period is during oogenetic stages 7--14.

(Boswell et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mago¹ has sterile phenotype, suppressible by Cele\mag-1^mago.PN

(Newmark et al., 1997)

Enhancer of

Statement

Reference

mago¹/mago[+] is an enhancer of visible phenotype of rl¹

(Ashton-Beaucage et al., 2010)

mago¹/mago[+] is an enhancer of visible phenotype of csw^lf

(Ashton-Beaucage et al., 2010)

Suppressor of

Statement

Reference

mago¹/mago[+] is a suppressor of visible | dominant phenotype of Egfr^E1

(Ashton-Beaucage et al., 2010)

mago¹/mago[+] is a suppressor of visible phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

Other

Statement

Reference

Df(2R)F36/mago¹, mira^{Δ103.αTub67C.mGFP6} has abnormal planar polarity phenotype

(Irion et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3R)p-XT103, mago¹ has embryonic head phenotype, suppressible | partially by osk^+tMa

(Morais-de-Sá et al., 2013)

Df(3R)p-XT103, mago¹ has embryonic abdominal segment phenotype, suppressible | partially by osk^+tMa

(Morais-de-Sá et al., 2013)

Df(3R)p-XT103, mago¹ has embryo phenotype, suppressible | partially by osk^+tMa

(Morais-de-Sá et al., 2013)

Enhancer of

Statement

Reference

mago¹/mago[+] is an enhancer of eye phenotype of rl¹

(Ashton-Beaucage et al., 2010)

mago¹/mago[+] is an enhancer of wing vein phenotype of rl¹

(Ashton-Beaucage et al., 2010)

mago¹/mago[+] is an enhancer of wing vein phenotype of csw^lf

(Ashton-Beaucage et al., 2010)

Suppressor of

Statement

Reference

mago¹/mago[+] is a suppressor of wing vein | ectopic phenotype of Egfr^E1

(Ashton-Beaucage et al., 2010)

mago¹/mago[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

Other

Statement

Reference

Df(3R)p-XT103, mago¹ has embryo phenotype

(Morais-de-Sá et al., 2013)

Df(3R)p-XT103, mago¹ has embryonic abdominal segment phenotype

(Morais-de-Sá et al., 2013)

Df(3R)p-XT103, mago¹ has embryonic head phenotype

(Morais-de-Sá et al., 2013)

Df(2R)F36/mago¹, mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen phenotype

(Irion et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from mago¹/Df(3R)p-XT103 mutants exhibit abdominal and head defects (in up to 35% of embryos). The presence of one copy of osk^t.WT partially suppresses these defects while the presence of one copy of osk^S244A.S248A exacerbates these defects and increases the number of bicaudal embryos.

(Morais-de-Sá et al., 2013)

mago¹ dominantly suppresses the rough eye phenotype caused by Ras85D^V12.sev.

mago¹ dominantly enhances the loss of wing vein phenotype seen in csw^lf/Y flies.

The extra wing vein phenotype seen in Egfr^E1/+ flies is dominantly suppressed by mago¹.

The loss of wing veins and rough eye phenotype seen in rl¹ homozygotes is dominantly enhanced by mago¹.

(Ashton-Beaucage et al., 2010)

A mago¹ heterozygous background enhances eye degeneration found in Scer\GAL4^GMR.PF>cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} flies.

Wing phenotypes found with overexpression of cln3^Scer\UAS.cTa with Scer\GAL4^en-e16E are enhanced in a mago¹ heterozygous background.

(Tuxworth et al., 2009)

Embryos that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a mago¹/Df(2R)F36 background have no posterior abdomen and have an anterior abdomen of reversed polarity.

(Irion et al., 2006)

Xenogenetic Interactions

Statement

Reference

A single copy of Cele\mag-1^mago.PN is sufficient to rescue the axis defects and sterility caused by mago¹.

(Newmark et al., 1997)

Complementation and Rescue Data

Rescued by

mago¹ is rescued by mago^GFP

(Newmark et al., 1997)

mago¹ is rescued by mago^Tag:MYC

(Newmark et al., 1997)

mago¹ is rescued by mago^+t2.2

(Newmark and Boswell, 1994)

Comments

Defects in axis formation and germ-plasm assembly caused by mago¹ are rescued by mago^{+t2.2.T:Hsap\MYC} and also by mago^{+t2.2.T:Avic\GFP}.

(Newmark et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

(Bardsley et al., 1993)

Shows interallelic complementation for zygotic lethality with mago^E19A and mago^WE7.

(Newmark and Boswell, 1994)

Germ line clonal analysis indicates that mago function is required in the germ line.

(Micklem et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mago¹

(Morais-de-Sá et al., 2013, Ashton-Beaucage et al., 2010, Tuxworth et al., 2009, Zimyanin et al., 2008, Doerflinger et al., 2006, Irion et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (26)

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