FlyBase Allele Report: Dmel\mira[Δ103.αTub67C.mGFP6]

General Information

Symbol

Dmel\mira^{Δ103.αTub67C.mGFP6}

Species

D. melanogaster

Name

FlyBase ID

FBal0197532

Feature type

allele

Associated gene

Dmel\mira

Associated Insertion(s)

Carried in Construct

P{tub-mira.Δ103.GFP}

Key Links

Transgenic product class

inframe_deletion

(Irion et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Irion et al., 2006)

Mutagen

in vitro construct

(Irion et al., 2006)

Progenitor genotype

Carried in construct

P{tub-mira.Δ103.GFP}

Cytology

Description

An αTub67C promoter drives expression of amino acids 1-727 of the mira coding region, tagged with mGFP6. The expressed mira is lacking the C-terminal 103 amino acids compared to the full-length protein.

(Irion et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

αTub67C

Encoded product / tool

mira

Tagged with

mGFP6

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | homeotic

(Irion et al., 2006)

Phenotype Manifest In

embryonic abdomen | ectopic

(Irion et al., 2006)

embryonic head

(Irion et al., 2006)

embryonic thorax

(Irion et al., 2006)

primordial germ cell | ectopic

(Irion et al., 2006)

Detailed Description

Statement

Reference

Expression of mira^{Δ103.αTub67C.T:Avic\GFP-m6} results in embryos that have an anterior abdomen in place of the head and thorax with 100% penetrance. These embryos also have ectopic anterior pole cells.

(Irion et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mira^{Δ103.αTub67C.mGFP6} has visible | homeotic phenotype, suppressible by swa³/swa¹

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has visible | homeotic phenotype, suppressible by exu^VL/exu⁴

(Irion et al., 2006)

NOT suppressed by

Statement

Reference

mira^{Δ103.αTub67C.mGFP6} has visible | homeotic phenotype, non-suppressible by Sry-β::Sry-δ^DB56/Sry-δ¹⁴/Df(3R)X3F

(Irion et al., 2006)

Other

Statement

Reference

Df(2R)F36/mago¹, mira^{Δ103.αTub67C.mGFP6} has abnormal planar polarity phenotype

(Irion et al., 2006)

Tm1^unspecified, mira^{Δ103.αTub67C.mGFP6} has abnormal planar polarity phenotype

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6}, stau^{Δloop2.αTub67C.Tag:MYC}, stau² has abnormal planar polarity phenotype

(Irion et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen | ectopic phenotype, suppressible by exu^VL/exu⁴

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has embryonic thorax phenotype, suppressible by exu^VL/exu⁴

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen | ectopic phenotype, suppressible by swa³/swa¹

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has embryonic thorax phenotype, suppressible by swa³/swa¹

(Irion et al., 2006)

NOT suppressed by

Statement

Reference

mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen | ectopic phenotype, non-suppressible by Sry-β::Sry-δ^DB56/Sry-δ¹⁴/Df(3R)X3F

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has embryonic head phenotype, non-suppressible by Sry-β::Sry-δ^DB56/Sry-δ¹⁴/Df(3R)X3F

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has embryonic thorax phenotype, non-suppressible by Sry-β::Sry-δ^DB56/Sry-δ¹⁴/Df(3R)X3F

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6} has primordial germ cell | ectopic phenotype, non-suppressible by Sry-β::Sry-δ^DB56/Sry-δ¹⁴/Df(3R)X3F

(Irion et al., 2006)

Other

Statement

Reference

exu^VL/exu⁴, mira^{Δ103.αTub67C.mGFP6} has embryonic head phenotype

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6}, swa³/swa¹ has embryonic head phenotype

(Irion et al., 2006)

Df(2R)F36/mago¹, mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen phenotype

(Irion et al., 2006)

Tm1^unspecified, mira^{Δ103.αTub67C.mGFP6} has embryonic abdomen phenotype

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6}, stau^{Δloop2.αTub67C.Tag:MYC}, stau² has embryonic abdomen phenotype

(Irion et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Females that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a exu^VL/exu⁴ background produce embryos with an exu phenotype, in which the thorax and abdomen develop normally, but the head is absent.

Embryos from females expressing mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a swa¹/swa³ background show the swa¹/swa³ phenotype, in which the embryonic head is reduced but not lost.

Embryos that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} and Sry-β::Sry-δ^DB56 in a Sry-δ¹⁴/Df(3R)X3F background show that same phenotype as embryos that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a wild-type background.

Embryos that express both mira^{Δ103.αTub67C.T:Avic\GFP-m6} and stau^{Δloop2.αTub67C.T:Hsap\MYC} in a stau² background lack a posterior abdomen and form an anterior abdomen with complete reverse polarity.

Embryos that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a mago¹/Df(2R)F36 background have no posterior abdomen and have an anterior abdomen of reversed polarity. Embryos that express mira^{Δ103.αTub67C.T:Avic\GFP-m6} in a Tm1^unspecified background show an identical reversed polarity phenotype.

(Irion et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mira^{Δ103.αTub67C.T:Avic\GFP-m6}

mira^{Δ103.αTub67C.mGFP6}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close