FlyBase Allele Report: Dmel\stau[2]

General Information

Symbol

Dmel\stau²

Species

D. melanogaster

Name

FlyBase ID

FBal0016165

Feature type

allele

Associated gene

Dmel\stau

Associated Insertion(s)

Carried in Construct

Also Known As

stau^D3, staufen^D3

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Broadus et al., 1998)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Deletion of approximately 250bp, approximately 0.8kb from the site of the P{ry11}stau^ry9 insertion.

(St. Johnston et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | heat sensitive (with stau³)

(Dubnau et al., 2003)

female sterile | maternal effect

(Hay et al., 1990)

lethal | maternal effect | recessive

(Lehmann and Nusslein-Volhard, 1991)

male sterile | maternal effect

(Hay et al., 1990)

Phenotype Manifest In

embryonic abdomen

(Ramos et al., 2000)

embryonic head

(Ramos et al., 2000)

primordial germ cell

(Ramos et al., 2000)

Detailed Description

Statement

Reference

Heterozygous stau² flies show normal 1-d memory after spaced or massed training.

(Bolduc et al., 2008)

stau³/stau² exhibit severe defects in one-day memory after spaced training when raised at 29^oC, but are similar to wild-type at 18^oC.

(Dubnau et al., 2003)

Ooplasmic streaming is normal at stage 9 and stage 10b in mutant oocytes.

(Palacios and St. Johnston, 2002)

In stau² embryos the NB6-4T-derived are present in most glial cell, one cell is missing in the remaining cases.

(Ragone et al., 2001)

Prevents the posterior accumulation of G-iα65A protein.

(Wolfgang and Forte, 1995)

hts RNA localization proceeds normally in embryos derived from stau mutant mothers.

(Ding et al., 1993)

stau²;6xP{osk⁺108} embryos exhibit a class of embryos with abdominal deletions typical of posterior group mutations.

(Smith et al., 1992)

Homozygotes have strong abdominal defects. stau²/stau³ heterozygotes at 18^oC have a weak abdominal defect, it is strong at 29^oC.

(Ephrussi et al., 1991)

Absence of posterior pole plasm, polar granules and pole cells.

(Lehmann and Nusslein-Volhard, 1991)

Embryos laid by stau² homozygous mothers form a bcd gradient in the anterior region of the embryo, this partial mislocalization leads to a shallower protein gradient.

(St. Johnston et al., 1989)

Slight reduction of the anteriormost region and defects in abdomen development. bcd protein levels are 50% that of wild type embryos.

(Driever and Nusslein-Volhard, 1988)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

stau² has abnormal memory phenotype, enhanceable by Fmr1³/Fmr1[+]

(Bolduc et al., 2008)

Enhancer of

Statement

Reference

stau²/stau[+] is an enhancer of abnormal memory phenotype of Fmr1³

(Bolduc et al., 2008)

stau² is an enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Suppressor of

Statement

Reference

stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau² is a suppressor of visible | homeotic phenotype of mira^{αTub67C.mGFP6}

(Irion et al., 2006)

Other

Statement

Reference

mira^{Δ103.αTub67C.mGFP6}, stau^{Δloop2.αTub67C.Tag:MYC}, stau² has abnormal planar polarity phenotype

(Irion et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

stau² has embryonic abdomen phenotype, enhanceable by neb^Mot

(Ruden et al., 1997)

Suppressed by

Statement

Reference

osk^ΔBRE, stau² has ventral denticle belt phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}

(Micklem et al., 2000)

osk^ΔBRE, stau² has embryonic epidermis phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}

(Micklem et al., 2000)

stau² has phenotype, suppressible by stau::Mdoa\stau^{DmMd.αTub67C.Tag:MYC}

(Micklem et al., 2000)

Enhancer of

Statement

Reference

stau²/stau[+] is an enhancer of ventral denticle belt | germline clone phenotype of Ge-1^Δ5

(Fan et al., 2011)

stau² is an enhancer of ommatidium phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

stau² is an enhancer of embryonic abdomen phenotype of neb^Mot

(Ruden et al., 1997)

Suppressor of

Statement

Reference

stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau² is a suppressor of embryonic abdomen | ectopic phenotype of mira^{αTub67C.mGFP6}

(Irion et al., 2006)

stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau² is a suppressor of embryonic head phenotype of mira^{αTub67C.mGFP6}

(Irion et al., 2006)

stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau² is a suppressor of embryonic thorax phenotype of mira^{αTub67C.mGFP6}

(Irion et al., 2006)

stau² is a suppressor of embryonic abdomen phenotype of osk^ΔBRE

(Micklem et al., 2000)

stau² is a suppressor of embryonic abdomen | ectopic phenotype of osk^ΔBRE

(Micklem et al., 2000)

Other

Statement

Reference

mira^{αTub67C.mGFP6}, stau^{ΔdRBD5.αTub67C.Tag:MYC}, stau² has embryonic abdomen phenotype

(Irion et al., 2006)

mira^{Δ103.αTub67C.mGFP6}, stau^{Δloop2.αTub67C.Tag:MYC}, stau² has embryonic abdomen phenotype

(Irion et al., 2006)

osk^ΔBRE, stau² has abdominal ventral denticle belt phenotype

(Micklem et al., 2000)

osk^ΔBRE, stau² has embryonic abdomen phenotype

(Micklem et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

stau²/+ increase the penetrance of the posterior patterning phenotype of embryos derived from Ge-1^Δ5 female germline clones from 4% to 32.8%.

(Fan et al., 2011)

Double heterozygous stau²/+; Fmr1³/+ mutant flies show defective 1-d memory after spaced training, but not after massed training.

(Bolduc et al., 2008)

Embryos that express both mira^{αTub67C.T:Avic\GFP-m6} and stau^{ΔdRBD5.αTub67C.T:Hsap\MYC} in a stau² background show the stau^{ΔdRBD5.αTub67C.T:Hsap\MYC} posterior group phenotype in which embryos develop a normal head and thorax, but lack the abdomen.

Embryos that express both mira^{Δ103.αTub67C.T:Avic\GFP-m6} and stau^{Δloop2.αTub67C.T:Hsap\MYC} in a stau² background lack a posterior abdomen and form an anterior abdomen with complete reverse polarity.

(Irion et al., 2006)

None of the embryos laid by stau², osk^ΔBRE mothers develop a bicaudal phenotype, and almost all form only one abdominal segment at the posterior. stau², osk^ΔBRE, stau^{ΔdRBD5.αTub67C.T:Hsap\MYC} mothers lay embryos with significantly more abdominal denticle belts than seen in stau²,osk^ΔBRE alone. A third of embryos laid by stau^{Δloop2.αTub67C.T:Hsap\MYC}, stau², osk^ΔBRE mothers develop a bicaudal phenotype, another third show suppression of head development and partial or complete rescue of the abdomen.

(Micklem et al., 2000)

Maternal-effect bicaudal phenotype is enhanced if mothers are also mutant for neb^Mot.

(Ruden et al., 1997)

Injection of nos^N5 RNA into homozygous embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

stau² is rescued by stau^{ΔN.αTub67C.Tag:MYC}

(Micklem et al., 2000)

Partially rescued by

stau² is partially rescued by stau^{ΔdRBD5.αTub67C.Tag:MYC}

(Micklem et al., 2000)

stau² is partially rescued by stau^{Δloop2.αTub67C.Tag:MYC}

(Micklem et al., 2000)

Not rescued by

stau² is not rescued by stau^dsRBD3mut

(Ramos et al., 2000)

Comments

The addition of stau^{ΔdRBD5.αTub67C.T:Hsap\MYC}, or stau^{Δloop2.αTub67C.T:Hsap\MYC} to stau² mothers, produces embryos that show almost no rescue of the stau² abdominal phenotype. The addition of stau^{ΔN.αTub67C.T:Hsap\MYC}, stau::Mdoa\stau^{DmMd.αTub67C.T:Hsap\MYC}, or stau^{αTub67C.T:Hsap\MYC} to stau² mothers rescues the phenotypes seen in eggs laid by stau² mothers.

(Micklem et al., 2000)

100% of stau^dsRBD3mut, stau² embryos die with head defects and no abdomen or pole cells.

(Ramos et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

86573

cn¹ stau²/CyO

86572

w^*; stau² PBac{αTub67C-stau.PB}VK00037/SM6a

86571

w^*; stau² PBac{αTub67C-stau.PA.mGFP6}VK00037/SM6a

86574

w^*; stau² PBac{αTub67C-stau.dsRBD3mut.mGFP6}VK00037/SM6a

86575

w^*; stau² PBac{αTub67C-stau.dsRBD4mut.mGFP6}VK00037/SM6a

Notes on Origin

Discoverer

Comments

hb protein expression in early stau² mutant embryos has been studied.

(Tautz, 1988)

Strong allele of stau. Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development.

(Lehmann and Nusslein-Volhard, 1991)

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

(Bardsley et al., 1993)

Transcripts from osk^olc1 behave as those of the endogenous osk gene.

(Kim-Ha et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

(Ramos et al., 2000)

stau²

stau^D3

(Gáspár et al., 2023, Lazzaretti et al., 2018, Lu et al., 2018, Morais-de-Sá et al., 2013, Sanghavi et al., 2012, Fan et al., 2011, Gonsalvez et al., 2010, Besse et al., 2009, Mhlanga et al., 2009, Bolduc et al., 2008, Irion and St Johnston, 2007, Irion and St Johnston, 2007, Snee et al., 2007, Irion et al., 2006, Lin et al., 2006, Munro et al., 2006, Weil et al., 2006, Braat et al., 2004, Huynh et al., 2004, Mutsuddi et al., 2004, Palacios and St. Johnston, 2002, Harris and Macdonald, 2001, van Eeden et al., 2001, Akiyama-Oda et al., 2000, Micklem et al., 2000, Shulman et al., 2000, Broadus et al., 1998, Matsuzaki et al., 1998, Schuldt et al., 1998, Chen et al., 1997, Glotzer et al., 1997, Li et al., 1997, Markussen et al., 1997, Ruden et al., 1997, Wilson et al., 1996, Markussen et al., 1995, Rongo et al., 1995, Wolfgang and Forte, 1995, Clark et al., 1994, Ferrandon et al., 1994, Irvine and Wieschaus, 1994, Jongens et al., 1994, Bardsley et al., 1993, St. Johnston, 1993, Akiyama and Okada, 1992, Jongens et al., 1992, Ephrussi et al., 1991, Kim-Ha et al., 1991, Lehmann and Nusslein-Volhard, 1991, Riddihough and Ish-Horowicz, 1991, St. Johnston et al., 1991, Wang and Lehmann, 1991, Grau and Gutzeit, 1990, Hay et al., 1990, Jack and McGinnis, 1990, St. Johnston et al., 1989, Driever and Nusslein-Volhard, 1988, Lehmann, 1988, Tearle and Nusslein-Volhard, 1987)

stauD3

(Dubnau et al., 2003)

staufen^D3

(Jankovics et al., 2001, Ragone et al., 2001, Tautz, 1988)

Name Synonyms

Secondary FlyBase IDs

References (69)

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