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General Information
Symbol
Dmel\Nicd.hs
Species
D. melanogaster
Name
intracellular domain
FlyBase ID
FBal0033872
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
HS-Nintra, hs-NICD, hs-Nintra
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Deletion of all sequences except the intracellular domain.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Depending on training protocol used, expression of Nicd.hs following heat shock in adults can impair long term memory formation.

Expression of Nicd.hs using heat shock in 0-8 hour old pupae results in the loss of most microchaetae on the notum.

Mutant embryos produce one eve expressing pericardial cell (EPC) and two myocardial cells per hemisegment.

Disrupts germ band retraction.

Expression of Nicd.hs produces adult eyes containing ommatidia with 4-5 outer cells and up to three R7-like cells. Some ommatidia have only one or two R7 photoreceptors.

37oC heat pulse at 24hr APF in pupae being raised at 29oC causes a mild double socket phenotype.

The number of eve-expressing pericardial cells is increased and DA1 muscles usually fail to form in embryos in which Nicd.hs is expressed using heat shock at late stage 11. A similar phenotype is seen in these embryos are also expressing numbfl.Scer\UAS.T:Hsap\MYC under the control of both Scer\GAL4how-24B and Scer\GAL4twi.PG.

60 minute heat shock at 37oX at 7-9 hours APF causes complete loss of microchaetes on the notum and shaft to socket transformation of macrochaetes. kuze29-4 mutant clones on the notum cause the formation of clusters of macrochaetes and microchaetes, microchaetae clusters are abolished by expression of Nicd.hs at 7-9 hours APF.

Strong antineurogenic phenotype that is independent of the N background.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

Nicd.hs has visible | heat sensitive phenotype, non-suppressible by edΔECD.UAS/Scer\GAL4sca-537.4

Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Nicd.hs has macrochaeta phenotype, enhanceable by Prosβ61

Nicd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ61

Nicd.hs has macrochaeta phenotype, enhanceable by Prosβ21

Nicd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ21

NOT suppressed by
Statement
Reference

Nicd.hs has microchaeta | heat sensitive phenotype, non-suppressible by edΔECD.UAS/Scer\GAL4sca-537.4

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Nicd.hs by performing daily heat-shocks for 5 days of adulthood significantly reduces the frequency of compound egg chamber formation in Ime4c1 mutant ovaries and allows progression through oogenesis past the previtellogenic stages.

The heat-shocked progeny from heterozygous amx1 females crossed to Nicd.hs males hatch at a reduced rate compared to wild type and only around 50% reach adulthood.

Fewer neurogenic phenotypes are seen in stage 8 embryos when homozygous amx1 mutant females are crossed to Nicd.hs males (heat-shocked before the first delamination round) than when amx1 females are crossed to wild type males.

The loss of microchaetae seen in animals expressing Nicd.hs using heat shock in 0-8 hour old pupae is not rescued by expression of edΔECD.Scer\UAS under the control of Scer\GAL4sca-537.4.

The Nicd.hs mild double socket phenotype is enhanced by Pros261 and Prosβ21. The Nicd.hs gene product is more stable in Pros261 expressing cells.

Fails to rescue the neurogenic phenotype of E(spl) mutants but rescues the neurogenic phenotype of bib mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Able to rescue the cuticle phenotype of N264-47 embryos.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid, transfected into S2 cells and used in biochemical assays.

Incomplete N protein.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Nicd.hs
S2-N-I
S2-Nintra
Name Synonyms
intracellular domain
Secondary FlyBase IDs
    References (21)