FlyBase Allele Report: Dmel\N[icd.hs]

General Information

Symbol

Dmel\N^icd.hs

Species

D. melanogaster

Name

intracellular domain

FlyBase ID

FBal0033872

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{hs-N.icd}

Also Known As

HS-Nintra, hs-N^intra, Notch<up>intracellular domain</up>

Key Links

Transgenic product class

inframe_deletion

(Lieber et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Lieber et al., 1993)

Mutagen

in vitro construct

(Lieber et al., 1993)

Progenitor genotype

Carried in construct

P{hs-N.icd}

Cytology

Description

An Hsp70 promoter drives expression of a minigene (contains genomic and cDNA sequence) that encodes the N intracellular domain (N residues from 1780 onwards are present). The encoded protein is constitutively active.

(Lieber et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | larval stage

(Frankenreiter et al., 2021)

visible | heat sensitive

(Escudero et al., 2003)

wild-type

(Lieber et al., 1993)

Phenotype Manifest In

campaniform sensillum of anterior crossvein | heat sensitive

(Lyman and Yedvobnick, 1995)

campaniform sensillum of dorsal radius | heat sensitive

(Lyman and Yedvobnick, 1995)

dorsal row of triple row of wing sensilla | heat sensitive

(Lyman and Yedvobnick, 1995)

embryonic/larval crystal cell | increased number | larval stage

(Frankenreiter et al., 2021)

external sensory organ | heat sensitive

(Lyman and Yedvobnick, 1995)

external sensory organ precursor cell IIa | ectopic | heat sensitive

(Gho and Schweisguth, 1998)

external sensory organ precursor cell IIb | heat sensitive

(Gho and Schweisguth, 1998)

interommatidial bristle | heat sensitive

(Lyman and Yedvobnick, 1995)

macrochaeta

(Schweisguth, 1999, Schweisguth et al., 1996)

macrochaeta | heat sensitive

(Lyman and Yedvobnick, 1995)

medial row of triple row of wing sensilla | heat sensitive

(Lyman and Yedvobnick, 1995)

microchaeta

(Schweisguth et al., 1996)

microchaeta | heat sensitive

(Escudero et al., 2003, Lyman and Yedvobnick, 1995)

muscle cell of A1-7 dorsal acute muscle 1

(Han and Bodmer, 2003)

muscle cell of mesothoracic dorsal acute muscle 1

(Han and Bodmer, 2003)

muscle cell of metathoracic dorsal acute muscle 1

(Han and Bodmer, 2003)

pericardial cell

(Han and Bodmer, 2003)

scutum & macrochaeta

(Pan and Rubin, 1997)

scutum & microchaeta

(Pan and Rubin, 1997)

sensory mother cell | heat sensitive

(Lyman and Yedvobnick, 1995)

tormogen cell | ectopic

(Schweisguth, 1999)

tormogen cell | heat sensitive

(Lyman and Yedvobnick, 1995)

trichome of the posterior wing margin | heat sensitive

(Lyman and Yedvobnick, 1995)

wing | heat sensitive

(Lyman and Yedvobnick, 1995)

wing vein | heat sensitive

(Lyman and Yedvobnick, 1995)

Detailed Description

Statement

Reference

Expression of N^icd.hs using heat shock in 0-8 hour old pupae results in the loss of most microchaetae on the notum.

(Escudero et al., 2003)

Mutant embryos produce one eve expressing pericardial cell (EPC) and two myocardial cells per hemisegment.

(Han and Bodmer, 2003)

37^oC heat pulse at 24hr APF in pupae being raised at 29^oC causes a mild double socket phenotype.

(Schweisguth, 1999)

The number of eve-expressing pericardial cells is increased and DA1 muscles usually fail to form in embryos in which N^icd.hs is expressed using heat shock at late stage 11. A similar phenotype is seen in these embryos are also expressing numb^{fl.Scer\UAS.T:Hsap\MYC} under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG.

(Park et al., 1998)

60 minute heat shock at 37^oX at 7-9 hours APF causes complete loss of microchaetes on the notum and shaft to socket transformation of macrochaetes. kuz^e29-4 mutant clones on the notum cause the formation of clusters of macrochaetes and microchaetes, microchaetae clusters are abolished by expression of N^icd.hs at 7-9 hours APF.

(Pan and Rubin, 1997)

Strong 'microchaetae loss' phenotype. At macrochaetae positions shafts are lost and extra sockets are present (shaft to socket cell transformation).

(Schweisguth et al., 1996)

Strong antineurogenic phenotype that is independent of the N background.

(Lieber et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

N^icd.hs has visible | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Suppressor of

Statement

Reference

N^icd.hs is a suppressor of neurogenic phenotype | maternal effect | embryonic stage 8 phenotype of amx¹

(Michellod and Randsholt, 2008)

Other

Statement

Reference

N^icd.hs, amx¹/amx[+] has partially lethal - majority live phenotype

(Michellod and Randsholt, 2008)

N^icd.hs, amx¹/amx[+] has majority die during embryonic stage phenotype

(Michellod and Randsholt, 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^icd.hs has macrochaeta phenotype, enhanceable by Prosβ6¹

(Schweisguth, 1999)

N^icd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ6¹

(Schweisguth, 1999)

N^icd.hs has macrochaeta phenotype, enhanceable by Prosβ2¹

(Schweisguth, 1999)

N^icd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ2¹

(Schweisguth, 1999)

Suppressed by

Statement

Reference

N^icd.hs has macrochaeta phenotype, suppressible by H^hs.PB

(Schweisguth et al., 1996)

N^icd.hs has microchaeta phenotype, suppressible by H^hs.PB

(Schweisguth et al., 1996)

N^icd.hs has wing margin | heat sensitive phenotype, suppressible | heat sensitive by H^hs.PB

(Lyman and Yedvobnick, 1995)

N^icd.hs has wing vein | heat sensitive phenotype, suppressible | heat sensitive by H^hs.PB

(Lyman and Yedvobnick, 1995)

N^icd.hs has interommatidial bristle | heat sensitive phenotype, suppressible by H^hs.PB

(Lyman and Yedvobnick, 1995)

NOT suppressed by

Statement

Reference

N^icd.hs has microchaeta | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Suppressor of

Statement

Reference

N^icd.hs is a suppressor of egg chamber phenotype of Mettl3^c1

(Hongay and Orr-Weaver, 2011)

N^icd.hs is a suppressor | heat sensitive of trichogen cell phenotype of Scer\GAL4^neur-P72, trbl^UAS.cMb

(Fichelson and Gho, 2004)

N^icd.hs is a suppressor of nervous system phenotype of bib^unspecified

(Lieber et al., 1993)

NOT Suppressor of

Statement

Reference

N^icd.hs is a non-suppressor of nervous system phenotype of E(spl)m8-HLH^unspecified

(Lieber et al., 1993)

Other

Statement

Reference

N^icd.hs, Scer\GAL4^neur-P72, trbl^UAS.cMb has external sensory organ precursor cell I phenotype

(Fichelson and Gho, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of N^icd.hs by performing daily heat-shocks for 5 days of adulthood significantly reduces the frequency of compound egg chamber formation in Ime4^c1 mutant ovaries and allows progression through oogenesis past the previtellogenic stages.

(Hongay and Orr-Weaver, 2011)

The heat-shocked progeny from heterozygous amx¹ females crossed to N^icd.hs males hatch at a reduced rate compared to wild type and only around 50% reach adulthood.

Fewer neurogenic phenotypes are seen in stage 8 embryos when homozygous amx¹ mutant females are crossed to N^icd.hs males (heat-shocked before the first delamination round) than when amx¹ females are crossed to wild type males.

(Michellod and Randsholt, 2008)

The loss of microchaetae seen in animals expressing N^icd.hs using heat shock in 0-8 hour old pupae is not rescued by expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4.

(Escudero et al., 2003)

The N^icd.hs mild double socket phenotype is enhanced by Pros26¹ and Prosβ2¹. The N^icd.hs gene product is more stable in Pros26¹ expressing cells.

(Schweisguth, 1999)

Fails to rescue the neurogenic phenotype of E(spl) mutants but rescues the neurogenic phenotype of bib mutants.

(Lieber et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

N^icd.hs rescues N^264-47

(Lieber et al., 1993)

Comments

Able to rescue the cuticle phenotype of N^264-47 embryos.

(Lieber et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51661

w^*; P{hs-N.icd}2/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N^icd.hs

N^intra

(Remaud et al., 2008)

Name Synonyms

Nintra

(Schweisguth, 1999)

intracellular domain

(Lieber et al., 1993)

Secondary FlyBase IDs

References (19)

Report Sections

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