FlyBase Allele Report: Dmel\Sxl[f18]

General Information

Symbol

Dmel\Sxl^f18

Species

D. melanogaster

Name

FlyBase ID

FBal0034090

Feature type

allele

Associated gene

Dmel\Sxl

Associated Insertion(s)

Carried in Construct

Also Known As

sxl^fs3, Sxl^fs#3

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bae et al., 1994)

Mutagen

Progenitor genotype

Cytology

Description

Point mutation.

(Hager and Cline, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:7,084,598..7,084,598 [-]

Nucleotide change:

G7084598A

Amino acid change:

Reported amino acid change:

G?D

Comment:

The Sxl^f18 point mutation blocks the alternative splicing necessary to generate exon-10-encoded C-terminal versions of Sxl female transcripts and substitutes aspartic acid for glycine in the exon-8-encoded C-terminal isoforms.

(Evans and Cline, 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Evans and Cline, 2013, Hager and Cline, 1997, Bae et al., 1994)

female sterile (with T(1;1)SxlΔPm)

(Evans and Cline, 2013)

fertile (with Sxl^f18,f32)

(Evans and Cline, 2013)

viable (with Sxl^f18,f32)

(Evans and Cline, 2013)

Phenotype Manifest In

cystoblast

(Tseng et al., 2014)

female germline stem cell | germline clone | progressive

(Tseng et al., 2014)

germarium

(Tseng et al., 2014)

oocyte

(Bopp et al., 1999)

oocyte & spindle

(Bopp et al., 1999)

ovary

(Starr and Cline, 2002, Bae et al., 1994, Wei et al., 1994, Oliver et al., 1990)

Detailed Description

Statement

Reference

Sxl^f18 mutant female germline stem cells are rapidly lost from the niche by two weeks after clone induction.

Approximately 8% of Sxl^f18 mutant germaria contain cystoblasts with defects in differentiation.

(Tseng et al., 2014)

Sxl^f18,f32 is a dominant intragenic suppressor of recessive Sxl^fs3 female sterility. Sxl^f18,f32/Sxl^fs3 females are fully viable and fertile.

(Evans and Cline, 2013)

Recombination across the y-sn interval on the X chromosome of Sxl^M1,f3/Sxl^fs3 females is reduced compared to wild type.

(Sun and Cline, 2009)

77% of ovaries of homozygous females that are not infected with Wolbachia have no eggs, 20% of ovaries have 1-2 eggs and 3% of ovaries have 3-9 eggs. These oogenesis defects are partly rescued by Wolbachia infection; 20% of the ovaries of infected homozygous females have no eggs, 28% of ovaries have 1-2 eggs, 40% of ovaries have 3-9 eggs and 12% of ovaries have more than 10 eggs. 95% of ovaries of Sxl^fs3/Sxl^f1 females that are infected with Wolbachia have no eggs and 5% of ovaries have 1-2 eggs.

(Starr and Cline, 2002)

Sxl^f4/Sxl^fs3; Su(Sxl^fs)X2^X2/+ females show severely reduced recombination (no recombinant progeny are produced). The reduction in recombination is caused by the lack of wild-type Sxl activity rather than the presence of Su(Sxl^fs)X2^X2 (recombination is restored to almost wild-type values in Sxl^fs3/+ ; Su(Sxl^fs)46⁴⁶/+ females). A high frequency of non-disjunction is also seen in Sxl^f4/Sxl^fs3 ; Su(Sxl^fs)X2^X2/+ females. Sxl^f4/Sxl^fs3; Su(Sxl^fs)X2^X2/+ vitellogenic cysts show the same defects as Sxl^f4/Sxl^f4; Su(Sxl^fs)46⁴⁶/+ cysts. No chiasmata are present in stage 13-14 oocytes of Sxl^f4/Sxl^fs3 ; Su(Sxl^fs)X2^X2/+ females. Consequently, chromosomes are usually seen in an anaphase-like arrangement in the first division spindle. In many instances, chromosomes are arranged in two or three spindles instead of one.

(Bopp et al., 1999)

Tumorous ovaries.

(Bae et al., 1994)

Many more germ cells in ovarian tumors are positive for expression of male germline markers than in wild type testes, suggesting a relaxation of growth control, or accumulation of apical cells due to a developmental block.

(Wei et al., 1994)

Homozygous females have ovarian tumours.

(Oliver et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Sxl^f18 is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

NOT Suppressor of

Statement

Reference

Sxl^f18 is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Sxl^f18 is a non-suppressor of decreased fecundity | dominant | female phenotype of ovo^D2

(Oliver et al., 1990)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype of a reduction in recombination across the y-sn interval on the X chromosome that is seen in Sxl^M1,f3/Sxl^fs3 females is enhanced by otu¹⁷/+ ; there is a further reduction in map distance in the double mutants. In addition, the rate of X chromosome nondisjunction is increased eightfold.

(Sun and Cline, 2009)

The number of vitellogenic eggs produced by ovo^D2 heterozygous females is not increased by Sxl^f18.

(Oliver et al., 1990)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Sxl^f25,M1

(Dines et al., 1998)

Comments

Sxl^f18,f32 is a dominant intragenic suppressor of recessive Sxl^fs3 female sterility. Sxl^f18,f32/Sxl^fs3 females are fully viable and fertile.

(Evans and Cline, 2013)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

4594

y¹ Sxl^f18/FM7c

58490

y¹ w¹ cm¹ Sxl^f18; P{Sxl.+tCa}9A

Kyoto

107815

y¹ Sxl^f18/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sxl^f18

(Grmai et al., 2018, Cline, 2014.11.10, Evans and Cline, 2013, Sun and Cline, 2009, Starr and Cline, 2002, Bopp et al., 1999, Dines et al., 1998, Soper et al., 1998, Hager and Cline, 1997)

Sxl^fs#3

(Oliver et al., 1994, Oliver et al., 1990)

Sxl^fs3

(Tseng et al., 2014)

f18

(Evans and Cline, 2013)

sxl^fs3

(Mutsuddi et al., 2004)

Name Synonyms

Secondary FlyBase IDs

FBal0086747

References (16)

Report Sections

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