FlyBase Allele Report: Dmel\ovo[D2]

General Information

Symbol

Dmel\ovo^D2

Species

D. melanogaster

Name

FlyBase ID

FBal0013376

Feature type

allele

Associated gene

Dmel\ovo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Mevel-Ninio et al., 1996, Chou et al., 1993, Garfinkel et al., 1992, Lindsley and Zimm, 1992, Wei et al., 1991, Oliver et al., 1990)

Mutagen

ethyl methanesulfonate

(Garfinkel et al., 1992, Kerridge and Dura, 1982)

Progenitor genotype

Cytology

Description

Mutation creates a new in-frame AUG codon in the open reading frame in exon 2.

(Mevel-Ninio et al., 1996)

Nucleotide substitution: G1572A.

(Mevel-Ninio et al., 1996)

Amino acid replacement: V176M.

(Mevel-Ninio et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:5,064,953..5,064,953 [+]

Nucleotide change:

G5064953A

Reported nucleotide change:

G1572A

Amino acid change:

V305M | ovo-PB; V176M | ovo-PC; V305M | ovo-PE

Reported amino acid change:

V176M

(Mevel-Ninio et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage

(Oliver et al., 1990)

egg

(Lindsley and Zimm, 1992)

egg chamber

(Pauli et al., 1995, Wei et al., 1991, Oliver et al., 1990)

egg chorion

(Oliver et al., 1990)

germline cell | female

(Mevel-Ninio et al., 1996)

ovary

(Pauli et al., 1995, Oliver et al., 1990)

Detailed Description

Statement

Reference

Females carrying ovo^D2 in an ovo^-/+ background show dominant female sterility. Heterozygous females contain only a few eggs.

(Andrews et al., 1998)

The dominant ovo^D2 phenotype is significantly enhanced by one copy of Df(2R)vg135, Df(2R)stil-A or Df(2R)stil-B, but is not significantly enhanced by one copy of stil¹, stil⁴ or stil⁵. The dominant enhancement of the ovo^D2 phenotype by Df(2R)vg135 is partially suppressed by a transgene carrying stil⁺. This suggests that the strong interaction between Df(2R)vg135 and ovo^D2 is due to the deletion of two genes, one of which is stil.

(Pennetta and Pauli, 1997)

In heterozygous females oogenesis is arrested. In the presence of either P{ovo-lacZ.B} or P{ovoM-D1} fertility of heterozygous females is significantly improved, average number of eggs laid rises from 2 to 30 and 24, respectively. For P{ovo-lacZ.B} up to 10% of eggs laid develop to adults, for P{ovoM-D1} only a small fraction of eggs develop. P{ovoM373} does not detectably change the ovo^D2 phenotype.

(Mevel-Ninio et al., 1996)

Heterozygous females lay only very few eggs. Introduction of ovo^{B.T:Ecol\lacZ} significantly increases the number of eggs laid. Laid eggs do not show any signs of development.

(Mevel-Ninio et al., 1995)

Most egg chambers of ovo^D2/+ heterozygous ovaries arrest around oogenic stage 6 and very few vitellogenic oocytes are found. Two types of abnormal egg chambers occur. Pseudonurse cell chambers, where the egg chambers contain more than 15 nurse cells (though not usually more than 25) contain 0-2 oocyte nuclei. The second type of egg chamber contains largely undifferentiated germ cells, of indeterminate sex.

(Pauli et al., 1995)

Phenotypic category: svb⁺.

(Garfinkel et al., 1992)

Oogenesis in heterozygous females is mainly arrested prior to stage 6.

(Granadino et al., 1992)

Permeable eggs.

(Lindsley and Zimm, 1992)

Heterozygous females are fully sterile.

(Mevel-Ninio et al., 1991)

Most egg chambers arrest before vitellogenesis. Fertility is restored by the presence of two copies of ovo⁺.

(Wei et al., 1991)

Most of the egg chambers of heterozygous females fail to initiate vitellogenesis. The more advanced egg chambers often show a variety of abnormalities, such as permeability, flaccidity, absence of the anterior end of the chorion, fused dorsal appendages, egg chambers with more than 16 nuclei and occasionally, small ovarian tumours. Less than 30% of the wild-type number of eggs are laid per day. The soma is wild-type. Transheterozygotes with either ovo^M1, ovo^M2 or ovo^D1rv20 have germ cells resembling early spermatocytes.

(Oliver et al., 1990)

Mutant gene activity works in opposition to wild type activity. Viable. Male germ line is fertile, female germ line has permeable eggs.

(Oliver et al., 1987)

Heterozygous females are completely sterile, producing only a few eggs throughout their lifetime, all of which fail to develop.

(Kerridge and Dura, 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ovo^D2 has female sterile | dominant phenotype, suppressible by Mmus\Ovol2^A.hs

(Unezaki et al., 2004)

ovo^D2 has female sterile | dominant phenotype, suppressible by ovo::Bole\ovo^ODP

(Khila et al., 2003)

ovo^D2 has female sterile | dominant phenotype, suppressible by ovo::Bole\ovo^ODM

(Khila et al., 2003)

ovo^D2 has decreased fecundity | dominant | female phenotype, suppressible by Sxl^fLS

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, suppressible by Sxl^M1

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, suppressible by Sxl^f7,M1

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, suppressible by Sxl^fP7B0

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, suppressible by Sxl^fP7C2

(Oliver et al., 1990)

NOT suppressed by

Statement

Reference

ovo^D2 has decreased fecundity | dominant | female phenotype, non-suppressible by Sxl^fPb

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, non-suppressible by Sxl^f9

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, non-suppressible by Sxl^f2

(Oliver et al., 1990)

ovo^D2 has decreased fecundity | dominant | female phenotype, non-suppressible by Sxl^f18

(Oliver et al., 1990)

Phenotype Manifest In

Enhanced by

Statement

Reference

ovo^D2 has phenotype, enhanceable by snf¹

(Pauli et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

The ovo^D2/+ phenotype can be partially suppressed by alleles of Sxl, enhanced by snf¹ and enhanced by alleles of otu.

(Pauli et al., 1995)

The number of vitellogenic eggs produced by ovo^D2 heterozygous females is increased by Sxl^f7,M1, Sxl^fP7B0, Sxl^fP7C2, Sxl^fLS, Sxl^M1 or Sxl^f7,M1, but not Sxl^fPb, Sxl^f9, Sxl^f2 or Sxl^f18.

(Oliver et al., 1990)

Xenogenetic Interactions

Statement

Reference

The fraction of ovo^D2/+ females that produce eggs is increased if the females express Mmus\Zfp339^A.hs using a 1 hour heat shock at 37^oC; 16-68% of the heat shocked females produce eggs, compared to 4% of control ovo^D2/+ females. However, almost all the eggs laid by the ovo^D2/+ females carrying Mmus\Zfp339^A.hs fail to hatch.

(Unezaki et al., 2004)

The dominant female sterility of ovo^D2 can be fully or partially rescued by ovo::Bole\ovo^ODM or ovo::Bole\ovo^ODP, depending on the transgenic line used.

(Khila et al., 2003)

Complementation and Rescue Data

Rescued by

ovo^D2 is rescued by ovo^S-7.2

(Mevel-Ninio et al., 1991)

Comments

One copy of P{ovo.S-7.2} fully rescues the sterility of heterozygous females.

(Mevel-Ninio et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

1318

ovo^D2 v²⁴/C(1)DX, y¹ f¹

1843

ovo^D2 v²⁴ P{FRT(w^hs)}9-2/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Komitopoulou.

(Lindsley and Zimm, 1992)

Comments

Clonal analysis shows that the dominant female sterile effect of ovo^D2 is germ line autonomous.

(Kerridge and Dura, 1982)

Sxl alleles that have no known effect on the soma do not suppress the ovo^D2 phenotype.

(Oliver et al., 1990)

Intermediate ovo phenotype.

(Mevel-Ninio et al., 1991)

Partially suppresses the female-specific gonadal dysgenesis caused by hybrid dysgenesis at 27.5^oC.

(Wei et al., 1991)

Shows a dominant interaction with otu alleles: more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed.

(Pauli et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Fs(1)K1103

(Kerridge and Dura, 1982)

ovo^D2

(Popodi et al., 2010-)

ovoD2

(Khila et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (27)

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