FlyBase Allele Report: Dmel\U2af50[XR15]

General Information

Symbol

Dmel\U2af50^XR15

Species

D. melanogaster

Name

FlyBase ID

FBal0034123

Feature type

allele

Associated gene

Dmel\U2af50

Associated Insertion(s)

Carried in Construct

Also Known As

9-21^XR15

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Lindsley, 1993, Stanewsky et al., 1993)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsoi et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Blanchette et al., 2004)

lethal | recessive

(Rudner et al., 1998, Rudner et al., 1998)

lethal - all die before end of larval stage | recessive

(Stanewsky et al., 1993)

some die during larval stage | recessive

(Stanewsky et al., 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous U2af50^XR15 mutants display a temperature-sensitive phenotype when grown at a restrictive temperature (30^oC). The homozygous females lay eggs with abnormal eggshell morphology, and the resultant mutant embryos fail to develop into larvae.

(Blanchette et al., 2004)

Germline clone analysis demonstrates cell lethality.

(Stanewsky et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

When mothers heterozygous for both snf^J210 and U2af50^XR15 are crossed with Sxl^fP7B0 males, no significant reduction in viability is seen in Sxl^fP7B0/+ daughters.

(Nagengast et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

U2af50^XR15 is rescued by U2af50^+mBa

(Blanchette et al., 2004)

U2af50^XR15 is rescued by U2af50^D204N.ts

(Blanchette et al., 2004)

U2af50^XR15 is rescued by U2af50^S284Y.ts

(Blanchette et al., 2004)

U2af50^XR15 is rescued by U2af50^RS.7.final

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^ΔRS.true

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^ΔRS.final

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^+tS12

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^RS.7

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^ΔRS

(Rudner et al., 1998)

U2af50^XR15 is rescued by U2af50^Tag:HA

(Rudner et al., 1998)

Partially rescued by

U2af50^XR15 is partially rescued by U2af50^mut.2.Tag:HA

(Rudner et al., 1998)

U2af50^XR15 is partially rescued by U2af50^mut.3.Tag:HA

(Rudner et al., 1998)

Not rescued by

U2af50^XR15 is not rescued by U2af50^D150A.E151A

(Blanchette et al., 2004)

U2af50^XR15 is not rescued by U2af50^T160I

(Blanchette et al., 2004)

U2af50^XR15 is not rescued by U2af50^RS.7.extreme

(Rudner et al., 1998)

U2af50^XR15 is not rescued by U2af50^ΔRS.extreme

(Rudner et al., 1998)

U2af50^XR15 is not rescued by U2af50^mut.4.Tag:HA

(Rudner et al., 1998)

U2af50^XR15 is not rescued by U2af50^mut.1.Tag:HA

(Rudner et al., 1998)

Comments

U2af50^+mBa efficiently rescues the U2af50^XR15 deletion with a similar rescue percentage at both 25^oC and 29^oC. Crossing four transgenic lines containing U2af50^D204N.ts with U2af50^XR15 results in rescue of the U2af50^XR15 phenotype in two of the lines (P{U2af50.D204N.ts}1 and P{U2af50.D204N.ts}4), with an optimal rescue of 39% at 25^oC and 15% at 29^oC in one of the lines (P{U2af50.D204N.ts}1). Crossing three transgenic lines containing U2af50^S284Y.ts with U2af50^XR15 results in rescue of the U2af50^XR15 phenotype in two of the three lines (P{U2af50.S284Y.ts}1 and P{U2af50.S284Y.ts}2), with an optimal rescue of 96% at 25^oC and 49% at 29^oC in one of the lines (P{U2af50.S284Y.ts}2).

(Blanchette et al., 2004)

U2af38^ΔE18 U2af50^XR15 double mutants can be rescued by a combination of U2af50^+tS12 and U2af38^+t10 but not by a combination of U2af50^ΔRS and U2af38^ΔRS. Lethal combination could both be rescued by an identical synthetic RS domain by a combination of U2af50^RS.7 and U2af38^ΔRS.

(Rudner et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Stanewsky.

(Lindsley, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

9-21^XR15

(Rudner et al., 1998, Rudner et al., 1998)

U2AF50^XR15

(Tsoi et al., 2011)

U2af50^XR15

l(1)9-21^XR15

(Stanewsky et al., 1993)

l(1)9-21^Xr15

(Lindsley, 1993)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close